Advanced

Using Endogenous MicroRNA Expression Patterns to Visualize Neural Differentiation of Human Pluripotent Stem Cells

Kirkeby, Agnete LU ; Parmar, Malin LU and Jakobsson, Johan LU (2011) In Human Embryonic and Induced Pluripotent Stem Cells p.465-480
Abstract
Many existing protocols for neuronal differentiation of human pluripotent cells result in heterogeneous cell populations and unsynchronized differentiation, necessitating the development of methods for labeling specific cell populations. Here we describe how microRNA-regulated lentiviral vectors can be used to visualize specific cell populations by exploiting endogenous microRNA expression patterns. This strategy provides a useful tool for visualization and identification of neural progeny derived from human pluripotent stem cells. We provide detailed protocols for lentiviral transduction, neural differentiation, and subsequent analysis of human embryonic stem cells.
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
pluripotent stem cells, microRNA
in
Human Embryonic and Induced Pluripotent Stem Cells
editor
Ye, Kaiming and Jin, Sha
pages
465 - 480
publisher
Springer
ISBN
978-1-61779-266-3
language
English
LU publication?
yes
id
12b8cb59-b41b-41b1-a6cd-8173c3acd045
date added to LUP
2016-06-01 13:54:07
date last changed
2016-06-01 14:43:09
@misc{12b8cb59-b41b-41b1-a6cd-8173c3acd045,
  abstract     = {Many existing protocols for neuronal differentiation of human pluripotent cells result in heterogeneous cell populations and unsynchronized differentiation, necessitating the development of methods for labeling specific cell populations. Here we describe how microRNA-regulated lentiviral vectors can be used to visualize specific cell populations by exploiting endogenous microRNA expression patterns. This strategy provides a useful tool for visualization and identification of neural progeny derived from human pluripotent stem cells. We provide detailed protocols for lentiviral transduction, neural differentiation, and subsequent analysis of human embryonic stem cells.},
  author       = {Kirkeby, Agnete and Parmar, Malin and Jakobsson, Johan},
  editor       = {Ye, Kaiming  and Jin, Sha },
  isbn         = {978-1-61779-266-3},
  keyword      = {pluripotent stem cells,microRNA},
  language     = {eng},
  month        = {07},
  pages        = {465--480},
  publisher    = {ARRAY(0x981ea08)},
  series       = {Human Embryonic and Induced Pluripotent Stem Cells},
  title        = {Using Endogenous MicroRNA Expression Patterns to Visualize Neural Differentiation of Human Pluripotent Stem Cells},
  year         = {2011},
}