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Pattern of Accessory Regions and Invasive Disease Potential in Streptococcus pneumoniae.

Blomberg, C; Dagerhamn, J; Dahlberg, S; Browall, S; Fernebro, Josefin LU ; Albiger, Barbara LU ; Morfeldt, E; Normark, S and Henriques-Normark, B (2009) In Journal of Infectious Diseases 199. p.1032-1042
Abstract
Background. The invasive disease potential (IDP) of Streptococcus pneumoniae differs between serotypes, but the reason for this is unknown. Methods. A total of 47 pneumococcal isolates from 13 serotypes with different IDPs in humans that belonged to 37 multilocus sequence types were compared by whole genome microarrays and mutant analyses. Results. Approximately 34% of the genes were variable, including 95 genes previously shown by signature-tagged mutagenesis (STM) to be required for invasive disease in mice. Many variable genes were localized to 41 accessory regions (ARs), of which 24 contained genes previously identified by STM as required for invasive disease. Only AR6 and AR34 were preferentially found in isolates of serotypes with... (More)
Background. The invasive disease potential (IDP) of Streptococcus pneumoniae differs between serotypes, but the reason for this is unknown. Methods. A total of 47 pneumococcal isolates from 13 serotypes with different IDPs in humans that belonged to 37 multilocus sequence types were compared by whole genome microarrays and mutant analyses. Results. Approximately 34% of the genes were variable, including 95 genes previously shown by signature-tagged mutagenesis (STM) to be required for invasive disease in mice. Many variable genes were localized to 41 accessory regions (ARs), of which 24 contained genes previously identified by STM as required for invasive disease. Only AR6 and AR34 were preferentially found in isolates of serotypes with high IDPs. Neither AR6, which carries a gene previously identified by STM as required for invasive disease and encodes a 6-phospho-beta glucosidase, nor the putative adhesin expressed by AR34 was required for mouse virulence in TIGR4. Conclusions. Pneumococci possess a repertoire of ARs that differ between clones and even between isolates of the same clone. The ARs required for invasive disease in humans may be redundant, as no unique pattern distinguished the most invasive clones from others. The ARs that contained genes previously identified by STM as required for virulence in mice were frequently absent from invasive human isolates. Only 1 AR (AR6) was present in almost all isolates from the serotypes with the highest IDP (1, 4, and 7F), whereas it was missing from many others. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Infectious Diseases
volume
199
pages
1032 - 1042
publisher
Oxford University Press
external identifiers
  • WOS:000264056600015
  • PMID:19203261
  • Scopus:64449085206
ISSN
1537-6613
DOI
10.1086/597205
language
English
LU publication?
yes
id
a1a63f50-2755-4ba7-9081-3aa512922f68 (old id 1302769)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/19203261?dopt=Abstract
date added to LUP
2009-03-06 10:55:57
date last changed
2016-10-13 04:27:04
@misc{a1a63f50-2755-4ba7-9081-3aa512922f68,
  abstract     = {Background. The invasive disease potential (IDP) of Streptococcus pneumoniae differs between serotypes, but the reason for this is unknown. Methods. A total of 47 pneumococcal isolates from 13 serotypes with different IDPs in humans that belonged to 37 multilocus sequence types were compared by whole genome microarrays and mutant analyses. Results. Approximately 34% of the genes were variable, including 95 genes previously shown by signature-tagged mutagenesis (STM) to be required for invasive disease in mice. Many variable genes were localized to 41 accessory regions (ARs), of which 24 contained genes previously identified by STM as required for invasive disease. Only AR6 and AR34 were preferentially found in isolates of serotypes with high IDPs. Neither AR6, which carries a gene previously identified by STM as required for invasive disease and encodes a 6-phospho-beta glucosidase, nor the putative adhesin expressed by AR34 was required for mouse virulence in TIGR4. Conclusions. Pneumococci possess a repertoire of ARs that differ between clones and even between isolates of the same clone. The ARs required for invasive disease in humans may be redundant, as no unique pattern distinguished the most invasive clones from others. The ARs that contained genes previously identified by STM as required for virulence in mice were frequently absent from invasive human isolates. Only 1 AR (AR6) was present in almost all isolates from the serotypes with the highest IDP (1, 4, and 7F), whereas it was missing from many others.},
  author       = {Blomberg, C and Dagerhamn, J and Dahlberg, S and Browall, S and Fernebro, Josefin and Albiger, Barbara and Morfeldt, E and Normark, S and Henriques-Normark, B},
  issn         = {1537-6613},
  language     = {eng},
  pages        = {1032--1042},
  publisher    = {ARRAY(0x9f90168)},
  series       = {Journal of Infectious Diseases},
  title        = {Pattern of Accessory Regions and Invasive Disease Potential in Streptococcus pneumoniae.},
  url          = {http://dx.doi.org/10.1086/597205},
  volume       = {199},
  year         = {2009},
}