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Reproducibility of human epidermal growth factor receptor 2 analysis in primary breast cancer - A national survey performed at pathology departments in Sweden.

Rydén, Lisa LU ; Haglund, Monica; Bendahl, Pär-Ola LU ; Hatschek, Thomas; Kolaric, Aleksandra; Kovacs, Aniko; Olsson, Ann; Olsson, Hans; Forsare, Carina LU and Fernö, Mårten LU (2009) In Acta oncologica (Stockholm, Sweden) 48. p.860-866
Abstract
Background. HER2 is a treatment predictive factor for the effect of trastuzumab and associated with poor prognosis in breast cancer. The analysis of HER2 must be performed with good quality, with regard to both the immunohistochemical (IHC) and in situ hybridization (ISH) analysis. Material and methods. A tissue microarray (TMA) including 11 breast cancer samples was sent twice (once in 2005 and again in 2006) to 24 pathology departments in Sweden. A questionnaire was also sent to the departments in 2006. Results. With IHC, all departments reported the same results (0/1+ vs. 2+ vs. 3 + ) for three (2005) and six samples (2006). The mean kappa-value increased from 0.67 to 0.77, indicating a good reproducibility at both occasions. With... (More)
Background. HER2 is a treatment predictive factor for the effect of trastuzumab and associated with poor prognosis in breast cancer. The analysis of HER2 must be performed with good quality, with regard to both the immunohistochemical (IHC) and in situ hybridization (ISH) analysis. Material and methods. A tissue microarray (TMA) including 11 breast cancer samples was sent twice (once in 2005 and again in 2006) to 24 pathology departments in Sweden. A questionnaire was also sent to the departments in 2006. Results. With IHC, all departments reported the same results (0/1+ vs. 2+ vs. 3 + ) for three (2005) and six samples (2006). The mean kappa-value increased from 0.67 to 0.77, indicating a good reproducibility at both occasions. With fluorescence-ISH (FISH), the 11 departments using this technique reported the same results (amplified vs. normal) for nine (2005) and ten samples (2006). The mean kappa-value showed very good reproducibility both 2005 and 2006 (0.92 and 0.96, respectively). Based on the answers from the participating departments, the questionnaire revealed that 31% of primary breast cancer diagnosed in 2006 (n = 5 043) were 2+ /3+ . FISH analysis of 2+ confirmed 12% of the samples to be amplified. The corresponding figure for 3 + was 90%. In total, 14.3% of the samples were HER2 positive (2+ and amplified, or 3 + ). Discussion. The results obtained in this study indicate that the reproducibility for HER2 analysis is good (IHC) and very good (FISH) between the pathology departments in Sweden using TMA-based tumor samples. In 2006, 14.3% of invasive breast cancers were HER2 positive. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Acta oncologica (Stockholm, Sweden)
volume
48
pages
860 - 866
publisher
Taylor & Francis
external identifiers
  • WOS:000269177500008
  • PMID:19353340
  • Scopus:70350689943
ISSN
1651-226X
DOI
10.1080/02841860902862511
language
English
LU publication?
yes
id
6e077cb9-ca3f-4b65-adcd-c0d8dca8f33f (old id 1392298)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/19353340?dopt=Abstract
date added to LUP
2009-05-06 08:37:59
date last changed
2016-10-13 04:27:20
@misc{6e077cb9-ca3f-4b65-adcd-c0d8dca8f33f,
  abstract     = {Background. HER2 is a treatment predictive factor for the effect of trastuzumab and associated with poor prognosis in breast cancer. The analysis of HER2 must be performed with good quality, with regard to both the immunohistochemical (IHC) and in situ hybridization (ISH) analysis. Material and methods. A tissue microarray (TMA) including 11 breast cancer samples was sent twice (once in 2005 and again in 2006) to 24 pathology departments in Sweden. A questionnaire was also sent to the departments in 2006. Results. With IHC, all departments reported the same results (0/1+ vs. 2+ vs. 3 + ) for three (2005) and six samples (2006). The mean kappa-value increased from 0.67 to 0.77, indicating a good reproducibility at both occasions. With fluorescence-ISH (FISH), the 11 departments using this technique reported the same results (amplified vs. normal) for nine (2005) and ten samples (2006). The mean kappa-value showed very good reproducibility both 2005 and 2006 (0.92 and 0.96, respectively). Based on the answers from the participating departments, the questionnaire revealed that 31% of primary breast cancer diagnosed in 2006 (n = 5 043) were 2+ /3+ . FISH analysis of 2+ confirmed 12% of the samples to be amplified. The corresponding figure for 3 + was 90%. In total, 14.3% of the samples were HER2 positive (2+ and amplified, or 3 + ). Discussion. The results obtained in this study indicate that the reproducibility for HER2 analysis is good (IHC) and very good (FISH) between the pathology departments in Sweden using TMA-based tumor samples. In 2006, 14.3% of invasive breast cancers were HER2 positive.},
  author       = {Rydén, Lisa and Haglund, Monica and Bendahl, Pär-Ola and Hatschek, Thomas and Kolaric, Aleksandra and Kovacs, Aniko and Olsson, Ann and Olsson, Hans and Forsare, Carina and Fernö, Mårten},
  issn         = {1651-226X},
  language     = {eng},
  pages        = {860--866},
  publisher    = {ARRAY(0xc510548)},
  series       = {Acta oncologica (Stockholm, Sweden)},
  title        = {Reproducibility of human epidermal growth factor receptor 2 analysis in primary breast cancer - A national survey performed at pathology departments in Sweden.},
  url          = {http://dx.doi.org/10.1080/02841860902862511},
  volume       = {48},
  year         = {2009},
}