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Parameter estimation of protein chromatographic processes based on breakthrough curves

Persson, Patrik LU and Nilsson, Bernt LU (2002) 8th IFAC International Conference on Computer Applications in Biotechnology (CAB8) In Computer Applications in Biotechnology 2001 (CAB8) p.113-118
Abstract
A methodology for parameter estimation in chromatographic models is suggested in this paper, which is based on breakthrough curves from a given column set-up. Physical parameters are found in two subsequent experiments, one for the mobile phase behaviour and one for the stationary phase behaviour. The estimations are based on flow rate changes and multiple responses. Adsorption parameters are found also in two subsequent experiments, one for isotherm parameters and one for kinetics parameters. These estimations are based on concentration changes. The conclusion is that it is possible to estimate the parameters in a general rate model using nonlinear least square estimation and numerical simulation of the model. Copyright (C) 2001 IFAC.
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author
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
simulation, numerical methods, parameter estimation, chromatography, modelling
in
Computer Applications in Biotechnology 2001 (CAB8)
pages
113 - 118
publisher
Elsevier
conference name
8th IFAC International Conference on Computer Applications in Biotechnology (CAB8)
external identifiers
  • WOS:000177564700020
ISBN
0080436811
language
English
LU publication?
yes
id
6b127a33-27f4-4f4e-bb57-14c436ab3975 (old id 1407245)
date added to LUP
2009-06-02 16:54:59
date last changed
2016-09-23 15:15:44
@misc{6b127a33-27f4-4f4e-bb57-14c436ab3975,
  abstract     = {A methodology for parameter estimation in chromatographic models is suggested in this paper, which is based on breakthrough curves from a given column set-up. Physical parameters are found in two subsequent experiments, one for the mobile phase behaviour and one for the stationary phase behaviour. The estimations are based on flow rate changes and multiple responses. Adsorption parameters are found also in two subsequent experiments, one for isotherm parameters and one for kinetics parameters. These estimations are based on concentration changes. The conclusion is that it is possible to estimate the parameters in a general rate model using nonlinear least square estimation and numerical simulation of the model. Copyright (C) 2001 IFAC.},
  author       = {Persson, Patrik and Nilsson, Bernt},
  isbn         = {0080436811},
  keyword      = {simulation,numerical methods,parameter estimation,chromatography,modelling},
  language     = {eng},
  pages        = {113--118},
  publisher    = {ARRAY(0x8cd7588)},
  series       = {Computer Applications in Biotechnology 2001 (CAB8)},
  title        = {Parameter estimation of protein chromatographic processes based on breakthrough curves},
  year         = {2002},
}