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Expression levels of HMGA2 in adipocytic tumors correlate with morphologic and cytogenetic subgroups.

Bartuma, Hammurabi LU ; Panagopoulos, Ioannis LU ; Collin, Anna LU ; Trombetta, Domenico; Domanski, Henryk LU ; Mandahl, Nils LU and Mertens, Fredrik LU (2009) In Molecular Cancer 8(Jun 9).
Abstract
BACKGROUND: The HMGA2 gene encodes a protein that alters chromatin structure. Deregulation, typically through chromosomal rearrangements, of HMGA2 has an important role in the development of several mesenchymal neoplasms. These rearrangements result in the expression of a truncated protein lacking the acidic C-terminus, a fusion protein consisting of the AT-hook domains encoded by exons 1-3 and parts from another gene, or a full-length protein; loss of binding sites for regulatory microRNA molecules from the 3' untranslated region (UTR) of HMGA2 has been suggested to be a common denominator. METHODS: Seventy adipocytic tumors, representing different morphologic and cytogenetic subgroups, were analyzed by qRT-PCR to study the expression... (More)
BACKGROUND: The HMGA2 gene encodes a protein that alters chromatin structure. Deregulation, typically through chromosomal rearrangements, of HMGA2 has an important role in the development of several mesenchymal neoplasms. These rearrangements result in the expression of a truncated protein lacking the acidic C-terminus, a fusion protein consisting of the AT-hook domains encoded by exons 1-3 and parts from another gene, or a full-length protein; loss of binding sites for regulatory microRNA molecules from the 3' untranslated region (UTR) of HMGA2 has been suggested to be a common denominator. METHODS: Seventy adipocytic tumors, representing different morphologic and cytogenetic subgroups, were analyzed by qRT-PCR to study the expression status of HMGA2; 18 of these tumors were further examined by PCR to search for mutations or deletions in the 3'UTR. RESULTS: Type (full-length or truncated) and level of expression varied with morphology and karyotype, with the highest levels in atypical lipomatous tumors and lipomas with rearrangements of 12q13-15 and the lowest in lipomas with 6p- or 13q-rearrangements, hibernomas, spindle cell lipomas and myxoid liposarcomas. All 18 examined tumors showed reduced or absent expression of the entire, or parts of, the 3'UTR, which was not due to mutations at the DNA level. CONCLUSION: In adipocytic tumors with deregulated HMGA2 expression, the 3'UTR is consistently lost, either due to physical disruption of HMGA2 or a shift to production of shorter 3'UTR. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Molecular Cancer
volume
8
issue
Jun 9
publisher
BioMed Central
external identifiers
  • WOS:000268322400001
  • PMID:19508721
  • Scopus:67650081458
ISSN
1476-4598
DOI
10.1186/1476-4598-8-36
language
English
LU publication?
yes
id
46eb01c7-3040-4b69-befe-398906694f6e (old id 1434367)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/19508721?dopt=Abstract
date added to LUP
2009-07-03 14:43:47
date last changed
2016-10-13 04:29:53
@misc{46eb01c7-3040-4b69-befe-398906694f6e,
  abstract     = {BACKGROUND: The HMGA2 gene encodes a protein that alters chromatin structure. Deregulation, typically through chromosomal rearrangements, of HMGA2 has an important role in the development of several mesenchymal neoplasms. These rearrangements result in the expression of a truncated protein lacking the acidic C-terminus, a fusion protein consisting of the AT-hook domains encoded by exons 1-3 and parts from another gene, or a full-length protein; loss of binding sites for regulatory microRNA molecules from the 3' untranslated region (UTR) of HMGA2 has been suggested to be a common denominator. METHODS: Seventy adipocytic tumors, representing different morphologic and cytogenetic subgroups, were analyzed by qRT-PCR to study the expression status of HMGA2; 18 of these tumors were further examined by PCR to search for mutations or deletions in the 3'UTR. RESULTS: Type (full-length or truncated) and level of expression varied with morphology and karyotype, with the highest levels in atypical lipomatous tumors and lipomas with rearrangements of 12q13-15 and the lowest in lipomas with 6p- or 13q-rearrangements, hibernomas, spindle cell lipomas and myxoid liposarcomas. All 18 examined tumors showed reduced or absent expression of the entire, or parts of, the 3'UTR, which was not due to mutations at the DNA level. CONCLUSION: In adipocytic tumors with deregulated HMGA2 expression, the 3'UTR is consistently lost, either due to physical disruption of HMGA2 or a shift to production of shorter 3'UTR.},
  author       = {Bartuma, Hammurabi and Panagopoulos, Ioannis and Collin, Anna and Trombetta, Domenico and Domanski, Henryk and Mandahl, Nils and Mertens, Fredrik},
  issn         = {1476-4598},
  language     = {eng},
  number       = {Jun 9},
  publisher    = {ARRAY(0x9731f10)},
  series       = {Molecular Cancer},
  title        = {Expression levels of HMGA2 in adipocytic tumors correlate with morphologic and cytogenetic subgroups.},
  url          = {http://dx.doi.org/10.1186/1476-4598-8-36},
  volume       = {8},
  year         = {2009},
}