Advanced

Protozoan Acanthamoeba polyphaga as a potential reservoir for Campylobacter jejuni

Axelsson-Olsson, D; Waldenström, Jonas LU ; Broman, T; Olsen, B and Holmberg, M (2005) In Applied and Environmental Microbiology 71(2). p.987-992
Abstract
We showed by a laboratory experiment that four different Campylobacter jejuni strains are able to infect the protozoan Acanthamoeba polyphaga. C. jejuni cells survived for longer periods when cocultured with amoebae than when grown in culture alone. The infecting C. jejuni cells aggregated in amoebic vacuoles, in which they were seen to be actively moving. Furthermore, a resuscitation of bacterial cultures that were previously negative in culturability tests was observed after reinoculation into fresh amoeba cultures. After spontaneous rupture of the amoebae, C. jejuni could be detected by microscopy and culturability tests. Our results indicate that amoebae may serve as a nonvertebrate reservoir for C. jejuni in the environment.
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Applied and Environmental Microbiology
volume
71
issue
2
pages
987 - 992
publisher
American Society for Microbiology
external identifiers
  • PMID:15691957
  • WOS:000227043400050
  • Scopus:13544255424
ISSN
0099-2240
DOI
10.1128/AEM.71.2.987-992.2005
language
English
LU publication?
yes
id
d1c572c3-e773-406e-956e-17faa4061372 (old id 145152)
alternative location
http://dx.doi.org/10.1128/AEM.71.2.987-992.2005
date added to LUP
2007-06-21 14:51:42
date last changed
2016-12-04 04:42:31
@misc{d1c572c3-e773-406e-956e-17faa4061372,
  abstract     = {We showed by a laboratory experiment that four different Campylobacter jejuni strains are able to infect the protozoan Acanthamoeba polyphaga. C. jejuni cells survived for longer periods when cocultured with amoebae than when grown in culture alone. The infecting C. jejuni cells aggregated in amoebic vacuoles, in which they were seen to be actively moving. Furthermore, a resuscitation of bacterial cultures that were previously negative in culturability tests was observed after reinoculation into fresh amoeba cultures. After spontaneous rupture of the amoebae, C. jejuni could be detected by microscopy and culturability tests. Our results indicate that amoebae may serve as a nonvertebrate reservoir for C. jejuni in the environment.},
  author       = {Axelsson-Olsson, D and Waldenström, Jonas and Broman, T and Olsen, B and Holmberg, M},
  issn         = {0099-2240},
  language     = {eng},
  number       = {2},
  pages        = {987--992},
  publisher    = {ARRAY(0xafe2668)},
  series       = {Applied and Environmental Microbiology},
  title        = {Protozoan Acanthamoeba polyphaga as a potential reservoir for Campylobacter jejuni},
  url          = {http://dx.doi.org/10.1128/AEM.71.2.987-992.2005},
  volume       = {71},
  year         = {2005},
}