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A large scale method for preparation of plant thylakoids for use in body weight regulation.

Emek, Sinan C; Szilagyi, Anna; Akerlund, Hans-Erik; Albertsson, Per-Ake; Köhnke, Rickard LU ; Holm, Anders LU and Erlanson-Albertsson, Charlotte LU (2010) In Preparative Biochemistry & Biotechnology 40(1). p.13-27
Abstract
A method for preparation of thylakoids from plant leaves on a large scale is described. The method involves: 1) disruption of the cells with a blender followed by filtration to remove large cell debris and non disrupted cells. 2) precipitation of the thylakoids by adjusting the pH to the isoelectric point, pH 4.7. 3) a washing step by dilution of the precipitate in water followed by precipitation at the same pH. 4) concentration of the precipitate by freeze- thawing or freeze -drying to get the final product. The product is characterized, with respect to protein composition, by SDS-PAGE and mass-spectroscopy, the content of carotenoids, particularly the xanthophylls violaxanthin, antheraxanthin, and zeaxanthin. The thylakoid preparation... (More)
A method for preparation of thylakoids from plant leaves on a large scale is described. The method involves: 1) disruption of the cells with a blender followed by filtration to remove large cell debris and non disrupted cells. 2) precipitation of the thylakoids by adjusting the pH to the isoelectric point, pH 4.7. 3) a washing step by dilution of the precipitate in water followed by precipitation at the same pH. 4) concentration of the precipitate by freeze- thawing or freeze -drying to get the final product. The product is characterized, with respect to protein composition, by SDS-PAGE and mass-spectroscopy, the content of carotenoids, particularly the xanthophylls violaxanthin, antheraxanthin, and zeaxanthin. The thylakoid preparation has about the same capacity to inhibit pancreatic lipase/colipase activity as thylakoids prepared by standard laboratory methods using sucrose in the medium and centrifugation. In a study with mice, it was found that, when the thylakoids were added to the food over 32 days, they significantly reduced the body weight gain and the percentage body fat. The large scale method described here allows studies on the effect of thylakoids in appetite regulation on experimental animals in a longer lasting time and also on humans. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Preparative Biochemistry & Biotechnology
volume
40
issue
1
pages
13 - 27
publisher
Taylor & Francis
external identifiers
  • WOS:000272898900002
  • PMID:20024791
  • Scopus:72349092433
ISSN
1532-2297
DOI
10.1080/10826060903413057
language
English
LU publication?
yes
id
ec01c6c6-c053-4962-94b5-2bb010ef9179 (old id 1523430)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/20024791?dopt=Abstract
date added to LUP
2010-01-11 16:54:44
date last changed
2016-10-30 04:35:05
@misc{ec01c6c6-c053-4962-94b5-2bb010ef9179,
  abstract     = {A method for preparation of thylakoids from plant leaves on a large scale is described. The method involves: 1) disruption of the cells with a blender followed by filtration to remove large cell debris and non disrupted cells. 2) precipitation of the thylakoids by adjusting the pH to the isoelectric point, pH 4.7. 3) a washing step by dilution of the precipitate in water followed by precipitation at the same pH. 4) concentration of the precipitate by freeze- thawing or freeze -drying to get the final product. The product is characterized, with respect to protein composition, by SDS-PAGE and mass-spectroscopy, the content of carotenoids, particularly the xanthophylls violaxanthin, antheraxanthin, and zeaxanthin. The thylakoid preparation has about the same capacity to inhibit pancreatic lipase/colipase activity as thylakoids prepared by standard laboratory methods using sucrose in the medium and centrifugation. In a study with mice, it was found that, when the thylakoids were added to the food over 32 days, they significantly reduced the body weight gain and the percentage body fat. The large scale method described here allows studies on the effect of thylakoids in appetite regulation on experimental animals in a longer lasting time and also on humans.},
  author       = {Emek, Sinan C and Szilagyi, Anna and Akerlund, Hans-Erik and Albertsson, Per-Ake and Köhnke, Rickard and Holm, Anders and Erlanson-Albertsson, Charlotte},
  issn         = {1532-2297},
  language     = {eng},
  number       = {1},
  pages        = {13--27},
  publisher    = {ARRAY(0xac56398)},
  series       = {Preparative Biochemistry & Biotechnology},
  title        = {A large scale method for preparation of plant thylakoids for use in body weight regulation.},
  url          = {http://dx.doi.org/10.1080/10826060903413057},
  volume       = {40},
  year         = {2010},
}