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Longitudinal clearance of seasonal influenza A viral RNA measured by real-time polymerase chain reaction in patients identified at a hospital emergency department.

Nilsson, Anna C; Brytting, Maria; Serifler, Filiz; Björkman, Per LU ; Persson, Kenneth LU and Widell, Anders LU (2010) In Scandinavian Journal of Infectious Diseases 42. p.679-686
Abstract
Abstract To study influenza virus shedding during acute infection, viral load was longitudinally measured by quantitative PCR in nasal flocked swabs from patients with seasonal H3N2 influenza at a Swedish emergency department, including both hospitalized patients and outpatients. Influenza A was detected in 65/184 patients. Sampling was repeated every 3-4 days in 45 patients, with the aim of continuing sampling until day 12 after disease onset. Home visits were offered. Antibodies were measured on paired sera in 95/184 patients. Fifty percent of the patients remained polymerase chain reaction (PCR)-positive 8 days after disease onset in a Kaplan-Meier survival curve. The longest observed duration of viral shedding was 12 days. The average... (More)
Abstract To study influenza virus shedding during acute infection, viral load was longitudinally measured by quantitative PCR in nasal flocked swabs from patients with seasonal H3N2 influenza at a Swedish emergency department, including both hospitalized patients and outpatients. Influenza A was detected in 65/184 patients. Sampling was repeated every 3-4 days in 45 patients, with the aim of continuing sampling until day 12 after disease onset. Home visits were offered. Antibodies were measured on paired sera in 95/184 patients. Fifty percent of the patients remained polymerase chain reaction (PCR)-positive 8 days after disease onset in a Kaplan-Meier survival curve. The longest observed duration of viral shedding was 12 days. The average viral load was initially low, peaked on days 2-3 of disease and then declined. Viral decline results remained similar when all 15 (25%) oseltamivir-treated patients were excluded. Significant antibody titre changes were seen in all the 35 PCR verified cases with available paired sera and in 8 of the 58 patients with negative PCR tests on acute phase nasal samples. In conclusion, quantitative PCR testing indicated the presence of influenza virus for up to 12 days, which could have implications for disease transmission and infection control. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Scandinavian Journal of Infectious Diseases
volume
42
pages
679 - 686
publisher
Informa Healthcare
external identifiers
  • WOS:000282716000006
  • PMID:20507261
  • Scopus:77956036861
ISSN
1651-1980
DOI
10.3109/00365548.2010.486001
language
English
LU publication?
yes
id
5b0faa73-91d0-4b3d-b4b3-f1b03d54dd0a (old id 1609820)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/20507261?dopt=Abstract
date added to LUP
2010-06-02 14:55:31
date last changed
2016-10-13 04:25:10
@misc{5b0faa73-91d0-4b3d-b4b3-f1b03d54dd0a,
  abstract     = {Abstract To study influenza virus shedding during acute infection, viral load was longitudinally measured by quantitative PCR in nasal flocked swabs from patients with seasonal H3N2 influenza at a Swedish emergency department, including both hospitalized patients and outpatients. Influenza A was detected in 65/184 patients. Sampling was repeated every 3-4 days in 45 patients, with the aim of continuing sampling until day 12 after disease onset. Home visits were offered. Antibodies were measured on paired sera in 95/184 patients. Fifty percent of the patients remained polymerase chain reaction (PCR)-positive 8 days after disease onset in a Kaplan-Meier survival curve. The longest observed duration of viral shedding was 12 days. The average viral load was initially low, peaked on days 2-3 of disease and then declined. Viral decline results remained similar when all 15 (25%) oseltamivir-treated patients were excluded. Significant antibody titre changes were seen in all the 35 PCR verified cases with available paired sera and in 8 of the 58 patients with negative PCR tests on acute phase nasal samples. In conclusion, quantitative PCR testing indicated the presence of influenza virus for up to 12 days, which could have implications for disease transmission and infection control.},
  author       = {Nilsson, Anna C and Brytting, Maria and Serifler, Filiz and Björkman, Per and Persson, Kenneth and Widell, Anders},
  issn         = {1651-1980},
  language     = {eng},
  pages        = {679--686},
  publisher    = {ARRAY(0xc66d500)},
  series       = {Scandinavian Journal of Infectious Diseases},
  title        = {Longitudinal clearance of seasonal influenza A viral RNA measured by real-time polymerase chain reaction in patients identified at a hospital emergency department.},
  url          = {http://dx.doi.org/10.3109/00365548.2010.486001},
  volume       = {42},
  year         = {2010},
}