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Effects of clinical isolates of Pseudomonas aeruginosa on Staphylococcus epidermidis biofilm formation.

Pihl, Maria; Chávez de Paz, Luis E; Schmidtchen, Artur LU ; Svensäter, Gunnel and Davies, Julia (2010) In FEMS immunology and medical microbiology Jul 1. p.504-512
Abstract
Abstract Pseudomonas aeruginosa is often found in chronic infections, including cystic fibrosis lung infections and those related to chronic wounds and venous ulcers. At the latter sites, P. aeruginosa can be isolated together with Staphylococcus epidermidis, and we have therefore explored the effect of clinical isolates and laboratory strains of P. aeruginosa strains on colonization by S. epidermidis in dual-species biofilms. Biofilm formation was assayed using 16S rRNA FISH and confocal laser scanning microscopy. Among the six P. aeruginosa strains tested, one particular strain, denoted 14:2, exerted a significant inhibitory effect, and even after 6 h, S. epidermidis levels in dual-species biofilms were reduced by >85% compared with... (More)
Abstract Pseudomonas aeruginosa is often found in chronic infections, including cystic fibrosis lung infections and those related to chronic wounds and venous ulcers. At the latter sites, P. aeruginosa can be isolated together with Staphylococcus epidermidis, and we have therefore explored the effect of clinical isolates and laboratory strains of P. aeruginosa strains on colonization by S. epidermidis in dual-species biofilms. Biofilm formation was assayed using 16S rRNA FISH and confocal laser scanning microscopy. Among the six P. aeruginosa strains tested, one particular strain, denoted 14:2, exerted a significant inhibitory effect, and even after 6 h, S. epidermidis levels in dual-species biofilms were reduced by >85% compared with those without P. aeruginosa. Interestingly, strain 14:2 was found to be negative for classical virulence determinants including pyocyanin, elastase and alkaline protease. Therefore, we suggest that less virulent phenotypes of P. aeruginosa, which may develop over time in chronic infections, could counteract colonization by S. epidermidis, ensuring persistence and dominance by P. aeruginosa in the host micro-habitat. Further studies are required to explain the inhibitory effect on S. epidermidis, although extracellular polysaccharides produced by P. aeruginosa might play a role in this phenomenon. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
FEMS immunology and medical microbiology
volume
Jul 1
pages
504 - 512
publisher
Wiley-Blackwell
external identifiers
  • WOS:000279900400030
  • PMID:20579097
  • Scopus:77954640542
ISSN
1574-695X
DOI
10.1111/j.1574-695X.2010.00707.x
language
English
LU publication?
yes
id
8e110207-eeac-44f1-8239-6a6ca7d70a15 (old id 1625696)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/20579097?dopt=Abstract
date added to LUP
2010-07-01 22:44:18
date last changed
2016-10-13 04:25:19
@misc{8e110207-eeac-44f1-8239-6a6ca7d70a15,
  abstract     = {Abstract Pseudomonas aeruginosa is often found in chronic infections, including cystic fibrosis lung infections and those related to chronic wounds and venous ulcers. At the latter sites, P. aeruginosa can be isolated together with Staphylococcus epidermidis, and we have therefore explored the effect of clinical isolates and laboratory strains of P. aeruginosa strains on colonization by S. epidermidis in dual-species biofilms. Biofilm formation was assayed using 16S rRNA FISH and confocal laser scanning microscopy. Among the six P. aeruginosa strains tested, one particular strain, denoted 14:2, exerted a significant inhibitory effect, and even after 6 h, S. epidermidis levels in dual-species biofilms were reduced by >85% compared with those without P. aeruginosa. Interestingly, strain 14:2 was found to be negative for classical virulence determinants including pyocyanin, elastase and alkaline protease. Therefore, we suggest that less virulent phenotypes of P. aeruginosa, which may develop over time in chronic infections, could counteract colonization by S. epidermidis, ensuring persistence and dominance by P. aeruginosa in the host micro-habitat. Further studies are required to explain the inhibitory effect on S. epidermidis, although extracellular polysaccharides produced by P. aeruginosa might play a role in this phenomenon.},
  author       = {Pihl, Maria and Chávez de Paz, Luis E and Schmidtchen, Artur and Svensäter, Gunnel and Davies, Julia},
  issn         = {1574-695X},
  language     = {eng},
  pages        = {504--512},
  publisher    = {ARRAY(0xbce99b8)},
  series       = {FEMS immunology and medical microbiology},
  title        = {Effects of clinical isolates of Pseudomonas aeruginosa on Staphylococcus epidermidis biofilm formation.},
  url          = {http://dx.doi.org/10.1111/j.1574-695X.2010.00707.x},
  volume       = {Jul 1},
  year         = {2010},
}