Advanced

C-peptide in dried blood spots.

Johansson, Jesper; Becker, Charlotte LU ; Persson, Nils; Fex, Malin LU and Törn, Carina LU (2010) In Scandinavian Journal of Clinical and Laboratory Investigation 70. p.404-409
Abstract
Abstract The aim was to evaluate if the Dried Blood Spot (DBS)-technique can be used to analyse C-peptide. S-C-peptide and paired whole blood clotted on filters, dried, punched out and eluted were sampled from 198 healthy subjects. Six subjects with S-C-peptide values outside the reference range were excluded. A conversion formula using log-DBS-C-peptide was generated in a subset of 156 ( approximately 80%) subjects with predictions made using also storage time (eluates) and age of subjects: (log S-C-peptide = 1.696 + 1.367 log DBS-C-peptide + 0.058 (storage time/month) + 0.014 (age/10 years). This formula was cross validated into the original population. Using Bland-Altman plots, mean difference between converted log DBS-C-peptide and log... (More)
Abstract The aim was to evaluate if the Dried Blood Spot (DBS)-technique can be used to analyse C-peptide. S-C-peptide and paired whole blood clotted on filters, dried, punched out and eluted were sampled from 198 healthy subjects. Six subjects with S-C-peptide values outside the reference range were excluded. A conversion formula using log-DBS-C-peptide was generated in a subset of 156 ( approximately 80%) subjects with predictions made using also storage time (eluates) and age of subjects: (log S-C-peptide = 1.696 + 1.367 log DBS-C-peptide + 0.058 (storage time/month) + 0.014 (age/10 years). This formula was cross validated into the original population. Using Bland-Altman plots, mean difference between converted log DBS-C-peptide and log S-C-peptide at baseline was 0 and limits of agreements were -0.18 to +0.18. Mean difference between converted log DBS-C-peptide values after six months and log S-C-peptide value from baseline was -0.01 and limits of agreement were -0.20-0.19. The lowest value detected with the DBS-technique corresponded to serum C-peptide 0.44 nmol/L. We concluded that DBS-C-peptide can be used as a first line screening test to monitor normal beta cell function. C-peptide on filters remained stable for six months. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Scandinavian Journal of Clinical and Laboratory Investigation
volume
70
pages
404 - 409
publisher
Informa Healthcare
external identifiers
  • WOS:000282914400004
  • PMID:20602576
  • Scopus:77956644194
ISSN
1502-7686
DOI
10.3109/00365513.2010.501113
language
English
LU publication?
yes
id
3b157839-b3ec-4575-8d18-04e06ec1f8d3 (old id 1645292)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/20602576?dopt=Abstract
date added to LUP
2010-08-02 10:39:55
date last changed
2016-10-28 15:21:18
@misc{3b157839-b3ec-4575-8d18-04e06ec1f8d3,
  abstract     = {Abstract The aim was to evaluate if the Dried Blood Spot (DBS)-technique can be used to analyse C-peptide. S-C-peptide and paired whole blood clotted on filters, dried, punched out and eluted were sampled from 198 healthy subjects. Six subjects with S-C-peptide values outside the reference range were excluded. A conversion formula using log-DBS-C-peptide was generated in a subset of 156 ( approximately 80%) subjects with predictions made using also storage time (eluates) and age of subjects: (log S-C-peptide = 1.696 + 1.367 log DBS-C-peptide + 0.058 (storage time/month) + 0.014 (age/10 years). This formula was cross validated into the original population. Using Bland-Altman plots, mean difference between converted log DBS-C-peptide and log S-C-peptide at baseline was 0 and limits of agreements were -0.18 to +0.18. Mean difference between converted log DBS-C-peptide values after six months and log S-C-peptide value from baseline was -0.01 and limits of agreement were -0.20-0.19. The lowest value detected with the DBS-technique corresponded to serum C-peptide 0.44 nmol/L. We concluded that DBS-C-peptide can be used as a first line screening test to monitor normal beta cell function. C-peptide on filters remained stable for six months.},
  author       = {Johansson, Jesper and Becker, Charlotte and Persson, Nils and Fex, Malin and Törn, Carina},
  issn         = {1502-7686},
  language     = {eng},
  pages        = {404--409},
  publisher    = {ARRAY(0x90ff3a8)},
  series       = {Scandinavian Journal of Clinical and Laboratory Investigation},
  title        = {C-peptide in dried blood spots.},
  url          = {http://dx.doi.org/10.3109/00365513.2010.501113},
  volume       = {70},
  year         = {2010},
}