C-peptide in dried blood spots.
(2010) In Scandinavian Journal of Clinical and Laboratory Investigation 70. p.404-409- Abstract
- Abstract The aim was to evaluate if the Dried Blood Spot (DBS)-technique can be used to analyse C-peptide. S-C-peptide and paired whole blood clotted on filters, dried, punched out and eluted were sampled from 198 healthy subjects. Six subjects with S-C-peptide values outside the reference range were excluded. A conversion formula using log-DBS-C-peptide was generated in a subset of 156 ( approximately 80%) subjects with predictions made using also storage time (eluates) and age of subjects: (log S-C-peptide = 1.696 + 1.367 log DBS-C-peptide + 0.058 (storage time/month) + 0.014 (age/10 years). This formula was cross validated into the original population. Using Bland-Altman plots, mean difference between converted log DBS-C-peptide and log... (More)
- Abstract The aim was to evaluate if the Dried Blood Spot (DBS)-technique can be used to analyse C-peptide. S-C-peptide and paired whole blood clotted on filters, dried, punched out and eluted were sampled from 198 healthy subjects. Six subjects with S-C-peptide values outside the reference range were excluded. A conversion formula using log-DBS-C-peptide was generated in a subset of 156 ( approximately 80%) subjects with predictions made using also storage time (eluates) and age of subjects: (log S-C-peptide = 1.696 + 1.367 log DBS-C-peptide + 0.058 (storage time/month) + 0.014 (age/10 years). This formula was cross validated into the original population. Using Bland-Altman plots, mean difference between converted log DBS-C-peptide and log S-C-peptide at baseline was 0 and limits of agreements were -0.18 to +0.18. Mean difference between converted log DBS-C-peptide values after six months and log S-C-peptide value from baseline was -0.01 and limits of agreement were -0.20-0.19. The lowest value detected with the DBS-technique corresponded to serum C-peptide 0.44 nmol/L. We concluded that DBS-C-peptide can be used as a first line screening test to monitor normal beta cell function. C-peptide on filters remained stable for six months. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1645292
- author
- Johansson, Jesper ; Becker, Charlotte LU ; Persson, Nils ; Fex, Malin LU and Törn, Carina LU
- organization
- publishing date
- 2010
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Scandinavian Journal of Clinical and Laboratory Investigation
- volume
- 70
- pages
- 404 - 409
- publisher
- Informa Healthcare
- external identifiers
-
- wos:000282914400004
- pmid:20602576
- scopus:77956644194
- ISSN
- 1502-7686
- DOI
- 10.3109/00365513.2010.501113
- language
- English
- LU publication?
- yes
- id
- 3b157839-b3ec-4575-8d18-04e06ec1f8d3 (old id 1645292)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/20602576?dopt=Abstract
- date added to LUP
- 2016-04-04 09:40:04
- date last changed
- 2022-01-29 18:59:34
@article{3b157839-b3ec-4575-8d18-04e06ec1f8d3,
abstract = {{Abstract The aim was to evaluate if the Dried Blood Spot (DBS)-technique can be used to analyse C-peptide. S-C-peptide and paired whole blood clotted on filters, dried, punched out and eluted were sampled from 198 healthy subjects. Six subjects with S-C-peptide values outside the reference range were excluded. A conversion formula using log-DBS-C-peptide was generated in a subset of 156 ( approximately 80%) subjects with predictions made using also storage time (eluates) and age of subjects: (log S-C-peptide = 1.696 + 1.367 log DBS-C-peptide + 0.058 (storage time/month) + 0.014 (age/10 years). This formula was cross validated into the original population. Using Bland-Altman plots, mean difference between converted log DBS-C-peptide and log S-C-peptide at baseline was 0 and limits of agreements were -0.18 to +0.18. Mean difference between converted log DBS-C-peptide values after six months and log S-C-peptide value from baseline was -0.01 and limits of agreement were -0.20-0.19. The lowest value detected with the DBS-technique corresponded to serum C-peptide 0.44 nmol/L. We concluded that DBS-C-peptide can be used as a first line screening test to monitor normal beta cell function. C-peptide on filters remained stable for six months.}},
author = {{Johansson, Jesper and Becker, Charlotte and Persson, Nils and Fex, Malin and Törn, Carina}},
issn = {{1502-7686}},
language = {{eng}},
pages = {{404--409}},
publisher = {{Informa Healthcare}},
series = {{Scandinavian Journal of Clinical and Laboratory Investigation}},
title = {{C-peptide in dried blood spots.}},
url = {{http://dx.doi.org/10.3109/00365513.2010.501113}},
doi = {{10.3109/00365513.2010.501113}},
volume = {{70}},
year = {{2010}},
}