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Intestinal damage in enterohemorrhagic Escherichia coli infection.

Békassy, Zivile LU ; Calderon Toledo, Carla LU ; Leoj, Gustav; Kristoffersson, Ann-Charlotte LU ; Leopold, Shana R; Perez, Maria Thereza LU and Karpman, Diana LU (2011) In Pediatric Nephrology oct. p.2059-2071
Abstract
Enterohemorrhagic Escherichia coli (EHEC) infection leads to marked intestinal injury. Sigmoid colon obtained from two children during EHEC infection exhibited abundant TUNEL-positive cells. To define which bacterial virulence factors contribute to intestinal injury the presence of Shiga toxin-2 (Stx2), intimin and the type III secretion system were correlated with symptoms and intestinal damage. C3H/HeN mice were inoculated with Stx2-producing (86-24) and non-producing (87-23) E. coli O157:H7 strains and 86-24 mutants lacking eae, encoding intimin (strain UMD619) or escN regulating the expression of type III secretion effectors (strain CVD451). Severe symptoms developed in mice inoculated with 86-24 and 87-23. Few mice inoculated with the... (More)
Enterohemorrhagic Escherichia coli (EHEC) infection leads to marked intestinal injury. Sigmoid colon obtained from two children during EHEC infection exhibited abundant TUNEL-positive cells. To define which bacterial virulence factors contribute to intestinal injury the presence of Shiga toxin-2 (Stx2), intimin and the type III secretion system were correlated with symptoms and intestinal damage. C3H/HeN mice were inoculated with Stx2-producing (86-24) and non-producing (87-23) E. coli O157:H7 strains and 86-24 mutants lacking eae, encoding intimin (strain UMD619) or escN regulating the expression of type III secretion effectors (strain CVD451). Severe symptoms developed in mice inoculated with 86-24 and 87-23. Few mice inoculated with the mutant strains developed severe symptoms. Strain 86-24 exhibited higher fecal bacterial counts, followed by 87-23, whereas strains UMD619 and CVD451 showed minimal fecal counts. More TUNEL-positive cells were found in proximal and distal colons of mice inoculated with strain 86-24 compared with strains 87-23 and CVD451 (p </= 0.01) or UMD619 (p < 0.05, proximal colon, p < 0.01, distal colon). The results show that strains 86-24 and 87-23 exhibited better colonic persistence and more symptoms, presumably due to the presence of intimin and type III secretion effectors. Extensive intestinal mucosal cell death was related to the presence of Stx2. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Pediatric Nephrology
volume
oct
pages
2059 - 2071
publisher
Springer
external identifiers
  • WOS:000295131900017
  • PMID:20809220
  • Scopus:83155184038
ISSN
1432-198X
DOI
10.1007/s00467-010-1616-9
language
English
LU publication?
yes
id
77957a8a-9d71-4371-a4f3-d1cb0a5089d2 (old id 1688642)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/20809220?dopt=Abstract
date added to LUP
2010-10-03 19:36:58
date last changed
2016-10-27 16:02:18
@misc{77957a8a-9d71-4371-a4f3-d1cb0a5089d2,
  abstract     = {Enterohemorrhagic Escherichia coli (EHEC) infection leads to marked intestinal injury. Sigmoid colon obtained from two children during EHEC infection exhibited abundant TUNEL-positive cells. To define which bacterial virulence factors contribute to intestinal injury the presence of Shiga toxin-2 (Stx2), intimin and the type III secretion system were correlated with symptoms and intestinal damage. C3H/HeN mice were inoculated with Stx2-producing (86-24) and non-producing (87-23) E. coli O157:H7 strains and 86-24 mutants lacking eae, encoding intimin (strain UMD619) or escN regulating the expression of type III secretion effectors (strain CVD451). Severe symptoms developed in mice inoculated with 86-24 and 87-23. Few mice inoculated with the mutant strains developed severe symptoms. Strain 86-24 exhibited higher fecal bacterial counts, followed by 87-23, whereas strains UMD619 and CVD451 showed minimal fecal counts. More TUNEL-positive cells were found in proximal and distal colons of mice inoculated with strain 86-24 compared with strains 87-23 and CVD451 (p &lt;/= 0.01) or UMD619 (p &lt; 0.05, proximal colon, p &lt; 0.01, distal colon). The results show that strains 86-24 and 87-23 exhibited better colonic persistence and more symptoms, presumably due to the presence of intimin and type III secretion effectors. Extensive intestinal mucosal cell death was related to the presence of Stx2.},
  author       = {Békassy, Zivile and Calderon Toledo, Carla and Leoj, Gustav and Kristoffersson, Ann-Charlotte and Leopold, Shana R and Perez, Maria Thereza and Karpman, Diana},
  issn         = {1432-198X},
  language     = {eng},
  pages        = {2059--2071},
  publisher    = {ARRAY(0xad0b418)},
  series       = {Pediatric Nephrology},
  title        = {Intestinal damage in enterohemorrhagic Escherichia coli infection.},
  url          = {http://dx.doi.org/10.1007/s00467-010-1616-9},
  volume       = {oct},
  year         = {2011},
}