Differences in islet-enriched miRNAs in healthy and glucose intolerant human subjects.
Bolmeson, Caroline LU ; Esguerra, Jonathan LU
; Salehi, S Albert
LU
; Speidel, Dina
LU
; Eliasson, Lena
LU
and Cilio, Corrado
LU
(2011)
In Biochemical and Biophysical Research Communications
Dec.
p.16-22
- Abstract
- Many microRNAs (miRNAs) are known to be cell-type specific and are implicated in development of diseases. We investigated the global expression pattern of miRNAs in human pancreatic islets compared to liver and skeletal muscle, using bead-based technology and quantitative RT-PCR. In addition to the known islet-specific miR-375, we also found enrichment of miR-127-3p, miR-184, miR-195 and miR-493∗ in the pancreatic islets. The expression of miR-375, miR-127-3p, miR-184 and the liver-enriched miR-122 were positively correlated to insulin biosynthesis, while the expression of miR-127-3p and miR-184 were negatively correlated to glucose-stimulated insulin secretion (GSIS). These correlations were absent in islets of glucose intolerant donors... (More)
- Many microRNAs (miRNAs) are known to be cell-type specific and are implicated in development of diseases. We investigated the global expression pattern of miRNAs in human pancreatic islets compared to liver and skeletal muscle, using bead-based technology and quantitative RT-PCR. In addition to the known islet-specific miR-375, we also found enrichment of miR-127-3p, miR-184, miR-195 and miR-493∗ in the pancreatic islets. The expression of miR-375, miR-127-3p, miR-184 and the liver-enriched miR-122 were positively correlated to insulin biosynthesis, while the expression of miR-127-3p and miR-184 were negatively correlated to glucose-stimulated insulin secretion (GSIS). These correlations were absent in islets of glucose intolerant donors (HbA1c⩾6.1). We suggest the presence of an islet-specific miRNA network, which consists of at least miR-375, miR-127-3p and miR-184, potentially involved in insulin secretion. Our results provide new insight into miRNA-mediated regulation of insulin secretion in healthy and glucose intolerant subjects. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1731717
- author
- Bolmeson, Caroline
LU
; Esguerra, Jonathan
LU
; Salehi, S Albert
LU
; Speidel, Dina
LU
; Eliasson, Lena
LU
and Cilio, Corrado
LU
- organization
- publishing date
- 2011
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Biochemical and Biophysical Research Communications
- volume
- Dec
- pages
- 16 - 22
- publisher
- Elsevier
- external identifiers
-
- wos:000286487700004
- pmid:21094635
- scopus:78650902669
- pmid:21094635
- ISSN
- 1090-2104
- DOI
- 10.1016/j.bbrc.2010.11.024
- language
- English
- LU publication?
- yes
- id
- 9ffc6bca-c591-4b85-a04b-a94f6b0eb346 (old id 1731717)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/21094635?dopt=Abstract
- date added to LUP
- 2016-04-04 09:08:17
- date last changed
- 2022-04-15 21:55:41
@article{9ffc6bca-c591-4b85-a04b-a94f6b0eb346,
abstract = {{Many microRNAs (miRNAs) are known to be cell-type specific and are implicated in development of diseases. We investigated the global expression pattern of miRNAs in human pancreatic islets compared to liver and skeletal muscle, using bead-based technology and quantitative RT-PCR. In addition to the known islet-specific miR-375, we also found enrichment of miR-127-3p, miR-184, miR-195 and miR-493∗ in the pancreatic islets. The expression of miR-375, miR-127-3p, miR-184 and the liver-enriched miR-122 were positively correlated to insulin biosynthesis, while the expression of miR-127-3p and miR-184 were negatively correlated to glucose-stimulated insulin secretion (GSIS). These correlations were absent in islets of glucose intolerant donors (HbA1c⩾6.1). We suggest the presence of an islet-specific miRNA network, which consists of at least miR-375, miR-127-3p and miR-184, potentially involved in insulin secretion. Our results provide new insight into miRNA-mediated regulation of insulin secretion in healthy and glucose intolerant subjects.}},
author = {{Bolmeson, Caroline and Esguerra, Jonathan and Salehi, S Albert and Speidel, Dina and Eliasson, Lena and Cilio, Corrado}},
issn = {{1090-2104}},
language = {{eng}},
pages = {{16--22}},
publisher = {{Elsevier}},
series = {{Biochemical and Biophysical Research Communications}},
title = {{Differences in islet-enriched miRNAs in healthy and glucose intolerant human subjects.}},
url = {{http://dx.doi.org/10.1016/j.bbrc.2010.11.024}},
doi = {{10.1016/j.bbrc.2010.11.024}},
volume = {{Dec}},
year = {{2011}},
}