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Chemical magnetoreception: Bird cryptochrome 1a is excited by blue light and forms long-lived radical-pairs

Liedvogel, Miriam LU ; Maeda, K.; Henbest, K.; Schleicher, E.; Simon, T.; Hore, P.J.; Timmel, C.R. and Mouritsen, H. (2007) In PLoS ONE
Abstract
Cryptochromes (Cry) have been suggested to form the basis of light-dependent magnetic compass orientation in birds. However, to function as magnetic compass sensors, the cryptochromes of migratory birds must possess a number of key biophysical characteristics. Most importantly, absorption of blue light must produce radical pairs with lifetimes longer than about a microsecond. Cryptochrome 1a (gwCry1a) and the photolyase-homology-region of Cry1 (gwCry1-PHR) from the migratory garden warbler were recombinantly expressed and purified from a baculovirus/Sf9 cell expression system. Transient absorption measurements show that these flavoproteins are indeed excited by light in the blue spectral range leading to the formation of radicals with... (More)
Cryptochromes (Cry) have been suggested to form the basis of light-dependent magnetic compass orientation in birds. However, to function as magnetic compass sensors, the cryptochromes of migratory birds must possess a number of key biophysical characteristics. Most importantly, absorption of blue light must produce radical pairs with lifetimes longer than about a microsecond. Cryptochrome 1a (gwCry1a) and the photolyase-homology-region of Cry1 (gwCry1-PHR) from the migratory garden warbler were recombinantly expressed and purified from a baculovirus/Sf9 cell expression system. Transient absorption measurements show that these flavoproteins are indeed excited by light in the blue spectral range leading to the formation of radicals with millisecond lifetimes. These biophysical characteristics suggest that gwCry1a is ideally suited as a primary light-mediated, radical-pair-based magnetic compass receptor. (Less)
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Public Library of Science
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  • Scopus:42549166971
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English
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yes
id
e75fa633-c494-49a1-8958-f3dc9b19dc23 (old id 1976908)
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http://www.plosone.org/article/info:doi/10.1371/journal.pone.0001106
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2011-06-14 16:27:20
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2016-11-27 04:34:01
@misc{e75fa633-c494-49a1-8958-f3dc9b19dc23,
  abstract     = {Cryptochromes (Cry) have been suggested to form the basis of light-dependent magnetic compass orientation in birds. However, to function as magnetic compass sensors, the cryptochromes of migratory birds must possess a number of key biophysical characteristics. Most importantly, absorption of blue light must produce radical pairs with lifetimes longer than about a microsecond. Cryptochrome 1a (gwCry1a) and the photolyase-homology-region of Cry1 (gwCry1-PHR) from the migratory garden warbler were recombinantly expressed and purified from a baculovirus/Sf9 cell expression system. Transient absorption measurements show that these flavoproteins are indeed excited by light in the blue spectral range leading to the formation of radicals with millisecond lifetimes. These biophysical characteristics suggest that gwCry1a is ideally suited as a primary light-mediated, radical-pair-based magnetic compass receptor.},
  author       = {Liedvogel, Miriam and Maeda, K. and Henbest, K. and Schleicher, E. and Simon, T. and Hore, P.J. and Timmel, C.R. and Mouritsen, H.},
  language     = {eng},
  publisher    = {ARRAY(0xa3d90d8)},
  series       = {PLoS ONE},
  title        = {Chemical magnetoreception: Bird cryptochrome 1a is excited by blue light and forms long-lived radical-pairs},
  year         = {2007},
}