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Aberrant class switching juxtaposes c-myc with a middle repetitive element (LINE) and an IgH intron in two spontaneously arising rat immunocytomas

Pear, W S; Nelson, S F; Axelson, H LU ; Wahlström, G; Bazin, H; Klein, G and Sümegi, J (1988) In Oncogene 2(5). p.499-507
Abstract

Our previous studies of spontaneously arising rat immunocytomas of the Lou/Wsl strain have shown that Ig switch regions are frequently the targets for c-myc recombination. In several tumors, however, we were unable to show recombination of the c-myc with Ig switch regions. We have cloned the rearranged c-myc fragments from 2 of these tumors, IR209 and IR223, and found that the c-myc recombines with a LINE region in the IR209 and with intron 1 of the epsilon locus in the IR223. Although switch regions are not found at the breakpoints, the sequences at the breakpoints share limited homology with Ig switch recognition sequences. This suggests that the switch recombinase enzymes are able to recognize sequences in addition to the defined... (More)

Our previous studies of spontaneously arising rat immunocytomas of the Lou/Wsl strain have shown that Ig switch regions are frequently the targets for c-myc recombination. In several tumors, however, we were unable to show recombination of the c-myc with Ig switch regions. We have cloned the rearranged c-myc fragments from 2 of these tumors, IR209 and IR223, and found that the c-myc recombines with a LINE region in the IR209 and with intron 1 of the epsilon locus in the IR223. Although switch regions are not found at the breakpoints, the sequences at the breakpoints share limited homology with Ig switch recognition sequences. This suggests that the switch recombinase enzymes are able to recognize sequences in addition to the defined switch recombination sites. At the same time, both the LINE and epsilon intron 1 sequences are located within the Ig cluster, providing further evidence for the selection of c-myc activation by Ig sequences in the pathogenesis of rat immunocytoma, mouse plasmacytoma, and Burkitt's lymphoma.

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publication status
published
keywords
Animals, Base Sequence, Genes, Immunoglobulin, Genes, Regulator, Genes, Switch, Immunoglobulin Heavy Chains, Immunoglobulin epsilon-Chains, Introns, Molecular Sequence Data, Plasmacytoma, Proto-Oncogene Proteins, Proto-Oncogenes, Rats, Repetitive Sequences, Nucleic Acid, Sequence Homology, Nucleic Acid, Translocation, Genetic
in
Oncogene
volume
2
issue
5
pages
9 pages
publisher
Nature Publishing Group
external identifiers
  • Scopus:0023902547
ISSN
0950-9232
language
English
LU publication?
no
id
1e64151a-5cb8-4c8f-b361-e1dc2e6253bb
date added to LUP
2016-08-09 09:21:26
date last changed
2016-09-15 11:04:45
@misc{1e64151a-5cb8-4c8f-b361-e1dc2e6253bb,
  abstract     = {<p>Our previous studies of spontaneously arising rat immunocytomas of the Lou/Wsl strain have shown that Ig switch regions are frequently the targets for c-myc recombination. In several tumors, however, we were unable to show recombination of the c-myc with Ig switch regions. We have cloned the rearranged c-myc fragments from 2 of these tumors, IR209 and IR223, and found that the c-myc recombines with a LINE region in the IR209 and with intron 1 of the epsilon locus in the IR223. Although switch regions are not found at the breakpoints, the sequences at the breakpoints share limited homology with Ig switch recognition sequences. This suggests that the switch recombinase enzymes are able to recognize sequences in addition to the defined switch recombination sites. At the same time, both the LINE and epsilon intron 1 sequences are located within the Ig cluster, providing further evidence for the selection of c-myc activation by Ig sequences in the pathogenesis of rat immunocytoma, mouse plasmacytoma, and Burkitt's lymphoma.</p>},
  author       = {Pear, W S and Nelson, S F and Axelson, H and Wahlström, G and Bazin, H and Klein, G and Sümegi, J},
  issn         = {0950-9232},
  keyword      = {Animals,Base Sequence,Genes, Immunoglobulin,Genes, Regulator,Genes, Switch,Immunoglobulin Heavy Chains,Immunoglobulin epsilon-Chains,Introns,Molecular Sequence Data,Plasmacytoma,Proto-Oncogene Proteins,Proto-Oncogenes,Rats,Repetitive Sequences, Nucleic Acid,Sequence Homology, Nucleic Acid,Translocation, Genetic},
  language     = {eng},
  number       = {5},
  pages        = {499--507},
  publisher    = {ARRAY(0xa214e28)},
  series       = {Oncogene},
  title        = {Aberrant class switching juxtaposes c-myc with a middle repetitive element (LINE) and an IgH intron in two spontaneously arising rat immunocytomas},
  volume       = {2},
  year         = {1988},
}