Advanced

Specificity and redundancy in the olfactory system of Ips typographus: Single-cell responses to ecologically relevant odors.

Andersson, Martin N LU ; Larsson, Mattias C and Schlyter, Fredrik (2009) In Journal of Insect Physiology 55. p.556-567
Abstract
We screened 150 olfactory sensilla in single-sensillum recordings to unravel the mechanisms underlying

host selection in the spruce bark beetle, Ips typographus (Coleoptera: Curculionidae: Scolytinae). Odor

stimuli comprised of pheromone (various bark beetle spp.), host, and non-host compounds elicited

strong and selective responses from 106 olfactory receptor neurons (ORNs), 45 of which were tuned to

pheromone compounds, 37 to host compounds, and 24 to non-host volatiles (NHV). In addition, 26 ORNs

responded only weakly to any odor stimulus. Strongly responding ORNs were classified into 17 classes.

Seven classes responded primarily to the Ips pheromone compounds: cis-verbenol, ipsenol,... (More)
We screened 150 olfactory sensilla in single-sensillum recordings to unravel the mechanisms underlying

host selection in the spruce bark beetle, Ips typographus (Coleoptera: Curculionidae: Scolytinae). Odor

stimuli comprised of pheromone (various bark beetle spp.), host, and non-host compounds elicited

strong and selective responses from 106 olfactory receptor neurons (ORNs), 45 of which were tuned to

pheromone compounds, 37 to host compounds, and 24 to non-host volatiles (NHV). In addition, 26 ORNs

responded only weakly to any odor stimulus. Strongly responding ORNs were classified into 17 classes.

Seven classes responded primarily to the Ips pheromone compounds: cis-verbenol, ipsenol, ipsdienol

(two classes), 2-methyl-3-buten-2-ol, amitinol, or verbenone, respectively. Six classes responded to the

host compounds: a-pinene, myrcene, p-cymene, myrcene and p-cymene, 1,8-cineole, or D3-carene,

respectively. Four classes responded to NHV: 3-octanol, 1-octen-3-ol, trans-conophthorin, or

indiscriminately to the repellent green leaf volatiles (GLVs) 1-hexanol, Z3-hexen-1-ol and E2-hexen-

1-ol, respectively. Indiscriminate responses from GLV neurons might explain a behavioral redundancy

among these GLVs. This is the first description of individual bark beetle ORNs dedicated to NHV

perception. These comprise almost 25% of the strongly responding neurons, demonstrating that a large

proportion of the olfactory system is devoted to signals from plants that the insect avoids. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Single-sensillum recordings, Odor coding, Non-host volatiles, Olfactory receptor neuron, Host selection
in
Journal of Insect Physiology
volume
55
pages
556 - 567
publisher
Elsevier
external identifiers
  • Scopus:67349147087
ISSN
1879-1611
DOI
10.1016/j.jinsphys.2009.01.018
language
English
LU publication?
no
id
b6d60002-4a4e-4875-b912-bda6ec460976 (old id 2028270)
date added to LUP
2011-07-20 11:45:26
date last changed
2016-11-27 04:31:50
@misc{b6d60002-4a4e-4875-b912-bda6ec460976,
  abstract     = {We screened 150 olfactory sensilla in single-sensillum recordings to unravel the mechanisms underlying<br/><br>
host selection in the spruce bark beetle, Ips typographus (Coleoptera: Curculionidae: Scolytinae). Odor<br/><br>
stimuli comprised of pheromone (various bark beetle spp.), host, and non-host compounds elicited<br/><br>
strong and selective responses from 106 olfactory receptor neurons (ORNs), 45 of which were tuned to<br/><br>
pheromone compounds, 37 to host compounds, and 24 to non-host volatiles (NHV). In addition, 26 ORNs<br/><br>
responded only weakly to any odor stimulus. Strongly responding ORNs were classified into 17 classes.<br/><br>
Seven classes responded primarily to the Ips pheromone compounds: cis-verbenol, ipsenol, ipsdienol<br/><br>
(two classes), 2-methyl-3-buten-2-ol, amitinol, or verbenone, respectively. Six classes responded to the<br/><br>
host compounds: a-pinene, myrcene, p-cymene, myrcene and p-cymene, 1,8-cineole, or D3-carene,<br/><br>
respectively. Four classes responded to NHV: 3-octanol, 1-octen-3-ol, trans-conophthorin, or<br/><br>
indiscriminately to the repellent green leaf volatiles (GLVs) 1-hexanol, Z3-hexen-1-ol and E2-hexen-<br/><br>
1-ol, respectively. Indiscriminate responses from GLV neurons might explain a behavioral redundancy<br/><br>
among these GLVs. This is the first description of individual bark beetle ORNs dedicated to NHV<br/><br>
perception. These comprise almost 25% of the strongly responding neurons, demonstrating that a large<br/><br>
proportion of the olfactory system is devoted to signals from plants that the insect avoids.},
  author       = {Andersson, Martin N and Larsson, Mattias C and Schlyter, Fredrik},
  issn         = {1879-1611},
  keyword      = {Single-sensillum recordings,Odor coding,Non-host volatiles,Olfactory receptor neuron,Host selection},
  language     = {eng},
  pages        = {556--567},
  publisher    = {ARRAY(0x8e59f28)},
  series       = {Journal of Insect Physiology},
  title        = {Specificity and redundancy in the olfactory system of <i>Ips typographus</i>: Single-cell responses to ecologically relevant odors.},
  url          = {http://dx.doi.org/10.1016/j.jinsphys.2009.01.018},
  volume       = {55},
  year         = {2009},
}