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Effect of Varying Glucose Concentrations during In Vitro Maturation and Embryo Culture on Efficiency of In Vitro Embryo Production in Buffalo.

Kumar, Parveen LU ; Verma, A; Roy, B; Rajput, S; Ojha, S; Anand, S; Yadav, P; Arora, J; De, S and Goswami, Sl, et al. (2012) In Reproduction in domestic animals = Zuchthygiene 47. p.269-273
Abstract
This study was aimed to optimize glucose level at different stages of buffalo in vitro embryo production procedure. Three glucose levels (1.5, 5.6 and 10 mm) along with a control (0 mm) were used at three phases of in vitro fertilisation (IVF) procedure viz. in vitro maturation (IVM), in vitro culture (IVC-I) (12-72 hpi) and IVC-II (72 hpi to 7 dpi). Maturation rate of oocytes was found different under different glucose concentrations, and significantly more number of oocytes reached to MII under 5.6 mm glucose. The glucose levels at each phase (IVM, IVC-I and IVC-II) individually had significant effect on blastocyst rate, and the level used at one phase had significant effect on the outcome of next phase. Complete withdrawal of glucose... (More)
This study was aimed to optimize glucose level at different stages of buffalo in vitro embryo production procedure. Three glucose levels (1.5, 5.6 and 10 mm) along with a control (0 mm) were used at three phases of in vitro fertilisation (IVF) procedure viz. in vitro maturation (IVM), in vitro culture (IVC-I) (12-72 hpi) and IVC-II (72 hpi to 7 dpi). Maturation rate of oocytes was found different under different glucose concentrations, and significantly more number of oocytes reached to MII under 5.6 mm glucose. The glucose levels at each phase (IVM, IVC-I and IVC-II) individually had significant effect on blastocyst rate, and the level used at one phase had significant effect on the outcome of next phase. Complete withdrawal of glucose from any of these stages irrespective of concentrations used at subsequent stage/s resulted in significantly lower number of blastocysts. However, the changing levels of glucose had differential effects during different phases of IVF steps. The most prominent effect of glucose level was observed during IVM. The presence of 5.6 mm glucose at all stages was most effective to yield highest blastocyst rate in buffalo IVF system. (Less)
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publication status
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Reproduction in domestic animals = Zuchthygiene
volume
47
pages
269 - 273
publisher
Wiley-Blackwell
external identifiers
  • WOS:000301534200019
  • PMID:21762215
  • Scopus:84858450862
ISSN
1439-0531
DOI
10.1111/j.1439-0531.2011.01849.x
language
English
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yes
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d7d5998b-8aac-4cda-be16-45deb04ab9f9 (old id 2058593)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/21762215?dopt=Abstract
date added to LUP
2011-08-01 19:59:39
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2016-10-13 04:34:59
@misc{d7d5998b-8aac-4cda-be16-45deb04ab9f9,
  abstract     = {This study was aimed to optimize glucose level at different stages of buffalo in vitro embryo production procedure. Three glucose levels (1.5, 5.6 and 10 mm) along with a control (0 mm) were used at three phases of in vitro fertilisation (IVF) procedure viz. in vitro maturation (IVM), in vitro culture (IVC-I) (12-72 hpi) and IVC-II (72 hpi to 7 dpi). Maturation rate of oocytes was found different under different glucose concentrations, and significantly more number of oocytes reached to MII under 5.6 mm glucose. The glucose levels at each phase (IVM, IVC-I and IVC-II) individually had significant effect on blastocyst rate, and the level used at one phase had significant effect on the outcome of next phase. Complete withdrawal of glucose from any of these stages irrespective of concentrations used at subsequent stage/s resulted in significantly lower number of blastocysts. However, the changing levels of glucose had differential effects during different phases of IVF steps. The most prominent effect of glucose level was observed during IVM. The presence of 5.6 mm glucose at all stages was most effective to yield highest blastocyst rate in buffalo IVF system.},
  author       = {Kumar, Parveen and Verma, A and Roy, B and Rajput, S and Ojha, S and Anand, S and Yadav, P and Arora, J and De, S and Goswami, Sl and Datta, Tk},
  issn         = {1439-0531},
  language     = {eng},
  pages        = {269--273},
  publisher    = {ARRAY(0x96d3260)},
  series       = {Reproduction in domestic animals = Zuchthygiene},
  title        = {Effect of Varying Glucose Concentrations during In Vitro Maturation and Embryo Culture on Efficiency of In Vitro Embryo Production in Buffalo.},
  url          = {http://dx.doi.org/10.1111/j.1439-0531.2011.01849.x},
  volume       = {47},
  year         = {2012},
}