The bacterial (Vibrio alginolyticus) production of tetrodotoxin in the ribbon worm Lineus longissimus - Just a false positive?
(2016) In Marine Drugs 14(4).- Abstract
We test previous claims that the bacteria Vibrio alginolyticus produces tetrodotoxin (TTX) when living in symbiosis with the nemertean Lineus longissimus by a setup with bacteria cultivation for TTX production. Toxicity experiments on the shore crab, Carcinus maenas, demonstrated the presence of a paralytic toxin, but evidence from LC-MS and electrophysiological measurements of voltage-gated sodium channel-dependent nerve conductance in male Wistar rat tissue showed conclusively that this effect did not originate from TTX. However, a compound of similar molecular weight was found, albeit apparently non-toxic, and with different LC retention time and MS/MS fragmentation pattern than those of TTX. We conclude that C. maenas paralysis and... (More)
We test previous claims that the bacteria Vibrio alginolyticus produces tetrodotoxin (TTX) when living in symbiosis with the nemertean Lineus longissimus by a setup with bacteria cultivation for TTX production. Toxicity experiments on the shore crab, Carcinus maenas, demonstrated the presence of a paralytic toxin, but evidence from LC-MS and electrophysiological measurements of voltage-gated sodium channel-dependent nerve conductance in male Wistar rat tissue showed conclusively that this effect did not originate from TTX. However, a compound of similar molecular weight was found, albeit apparently non-toxic, and with different LC retention time and MS/MS fragmentation pattern than those of TTX. We conclude that C. maenas paralysis and death likely emanate from a compound
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- author
- Strand, Malin ; Hedström, Martin LU ; Seth, Henrik ; McEvoy, Eric G. ; Jacobsson, Erik ; Göransson, Ulf ; Andersson, Håkan S. and Sundberg, Per
- organization
- publishing date
- 2016-04-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Axonal conductance, LC-MS, Mucus, Nemertean, Tetrodotoxin, Vibrio
- in
- Marine Drugs
- volume
- 14
- issue
- 4
- article number
- 63
- publisher
- MDPI AG
- external identifiers
-
- pmid:27023570
- wos:000374588000002
- scopus:84962751120
- ISSN
- 1660-3397
- DOI
- 10.3390/md14040063
- language
- English
- LU publication?
- yes
- id
- 20d5b8d3-ff9b-413e-9c11-931d8359ac13
- date added to LUP
- 2016-05-27 12:54:42
- date last changed
- 2024-02-02 18:48:02
@article{20d5b8d3-ff9b-413e-9c11-931d8359ac13,
abstract = {{<p>We test previous claims that the bacteria Vibrio alginolyticus produces tetrodotoxin (TTX) when living in symbiosis with the nemertean Lineus longissimus by a setup with bacteria cultivation for TTX production. Toxicity experiments on the shore crab, Carcinus maenas, demonstrated the presence of a paralytic toxin, but evidence from LC-MS and electrophysiological measurements of voltage-gated sodium channel-dependent nerve conductance in male Wistar rat tissue showed conclusively that this effect did not originate from TTX. However, a compound of similar molecular weight was found, albeit apparently non-toxic, and with different LC retention time and MS/MS fragmentation pattern than those of TTX. We conclude that C. maenas paralysis and death likely emanate from a compound </p>}},
author = {{Strand, Malin and Hedström, Martin and Seth, Henrik and McEvoy, Eric G. and Jacobsson, Erik and Göransson, Ulf and Andersson, Håkan S. and Sundberg, Per}},
issn = {{1660-3397}},
keywords = {{Axonal conductance; LC-MS; Mucus; Nemertean; Tetrodotoxin; Vibrio}},
language = {{eng}},
month = {{04}},
number = {{4}},
publisher = {{MDPI AG}},
series = {{Marine Drugs}},
title = {{The bacterial (Vibrio alginolyticus) production of tetrodotoxin in the ribbon worm Lineus longissimus - Just a false positive?}},
url = {{http://dx.doi.org/10.3390/md14040063}},
doi = {{10.3390/md14040063}},
volume = {{14}},
year = {{2016}},
}