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Laser-induced fluorescence studies of normal and malignant tumour tissue of rat following intravenous injection of delta-amino levulinic acid

Johansson, Jonas LU ; Berg, R; Svanberg, Katarina LU and Svanberg, Sune LU (1997) In Lasers in Surgery and Medicine 20(3). p.272-279
Abstract
Background and Objective: Laser-induced fluorescence was studied in normal and tumour tissue of rat after intravenous injection of delta-amino levulinic acid (ALA). The aim of the study was to investigate the protoporphyrin IX accumulation in different tissue types in rat after systemically administered ALA. Study Design/Material and Methods: A malignant rat tumour and normal tissue from 13 different organs were investigated in eight rats. The rats were injected with two different ALA doses, 30 and 90 mg/kg b.w., and the investigations were performed at 10, 30, and 240 min after the injection. The fluorescence was recorded utilising an optical fibre based fluorosensor at 405 nm excitation. Results: Fluorescence spectra were recorded in the... (More)
Background and Objective: Laser-induced fluorescence was studied in normal and tumour tissue of rat after intravenous injection of delta-amino levulinic acid (ALA). The aim of the study was to investigate the protoporphyrin IX accumulation in different tissue types in rat after systemically administered ALA. Study Design/Material and Methods: A malignant rat tumour and normal tissue from 13 different organs were investigated in eight rats. The rats were injected with two different ALA doses, 30 and 90 mg/kg b.w., and the investigations were performed at 10, 30, and 240 min after the injection. The fluorescence was recorded utilising an optical fibre based fluorosensor at 405 nm excitation. Results: Fluorescence spectra were recorded in the 400-750 nm wavelength region including the dual-peaked PpIX fluorescence at about 635 and 705 nm, and the tissue autofluorescence peaking at about 500 nm, The maximum tumour build-up of PpIX was achieved already in less than 1 hr after ALA injection. The fluorescence demarcation between tumour and surrounding tissue was a factor of 7-8:1 after 30 min and decreased for longer retention times. The accumulation in 13 different organs was investigated and a particularly high PpIX build-up was found in stomach and intestine. Conclusions: Fluorescence detection following i.v. injection of ALA provides attractive diagnostics for the experimental tumour used, indicating clinical usefulness. (C) 1997 Wiley-Liss, Inc. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Lasers in Surgery and Medicine
volume
20
issue
3
pages
272 - 279
publisher
John Wiley & Sons
external identifiers
  • Scopus:0030997011
ISSN
0196-8092
DOI
10.1002/(SICI)1096-9101(1997)20:3<272::AID-LSM6>3.0.CO;2-N
language
English
LU publication?
yes
id
0287de27-c235-4034-acdf-7e64ca82b3f4 (old id 2258488)
date added to LUP
2012-02-20 20:54:20
date last changed
2016-10-13 04:27:57
@misc{0287de27-c235-4034-acdf-7e64ca82b3f4,
  abstract     = {Background and Objective: Laser-induced fluorescence was studied in normal and tumour tissue of rat after intravenous injection of delta-amino levulinic acid (ALA). The aim of the study was to investigate the protoporphyrin IX accumulation in different tissue types in rat after systemically administered ALA. Study Design/Material and Methods: A malignant rat tumour and normal tissue from 13 different organs were investigated in eight rats. The rats were injected with two different ALA doses, 30 and 90 mg/kg b.w., and the investigations were performed at 10, 30, and 240 min after the injection. The fluorescence was recorded utilising an optical fibre based fluorosensor at 405 nm excitation. Results: Fluorescence spectra were recorded in the 400-750 nm wavelength region including the dual-peaked PpIX fluorescence at about 635 and 705 nm, and the tissue autofluorescence peaking at about 500 nm, The maximum tumour build-up of PpIX was achieved already in less than 1 hr after ALA injection. The fluorescence demarcation between tumour and surrounding tissue was a factor of 7-8:1 after 30 min and decreased for longer retention times. The accumulation in 13 different organs was investigated and a particularly high PpIX build-up was found in stomach and intestine. Conclusions: Fluorescence detection following i.v. injection of ALA provides attractive diagnostics for the experimental tumour used, indicating clinical usefulness. (C) 1997 Wiley-Liss, Inc.},
  author       = {Johansson, Jonas and Berg, R and Svanberg, Katarina and Svanberg, Sune},
  issn         = {0196-8092},
  language     = {eng},
  number       = {3},
  pages        = {272--279},
  publisher    = {ARRAY(0xaf00660)},
  series       = {Lasers in Surgery and Medicine},
  title        = {Laser-induced fluorescence studies of normal and malignant tumour tissue of rat following intravenous injection of delta-amino levulinic acid},
  url          = {http://dx.doi.org/10.1002/(SICI)1096-9101(1997)20:3<272::AID-LSM6>3.0.CO;2-N},
  volume       = {20},
  year         = {1997},
}