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Perioperative Betamethasone Treatment Reduces Signs of Bladder Dysfunction in a Rat Model for Neurapraxia in Female Urogenital Surgery.

Castiglione, Fabio; Bergamini, Alice; Bettiga, Arianna; Bivalacqua, Trinity J; Benigni, Fabio; Strittmatter, Frank; Gandaglia, Giorgio; Rigatti, Patrizio; Montorsi, Francesco and Hedlund, Petter LU (2012) In European Urology 62(6). p.1076-1085
Abstract
BACKGROUND: Information on autonomic neurapraxia in female urogenital surgery is scarce, and a model to study it is not available. OBJECTIVE: To develop a model to study the impact of autonomic neurapraxia on bladder function in female rats, as well as to assess the effects of corticosteroid therapy on the recovery of bladder function in this model.



DESIGN, SETTING, AND PARTICIPANTS: Female Sprague-Dawley rats were subjected to bilateral pelvic nerve crush (PNC) and perioperatively treated with betamethasone or vehicle. Bladder function and morphology of bladder tissue were evaluated and compared with sham-operated rats.



OUTCOME MEASUREMENTS AND STATISTICAL ANALYSIS: Western blot,... (More)
BACKGROUND: Information on autonomic neurapraxia in female urogenital surgery is scarce, and a model to study it is not available. OBJECTIVE: To develop a model to study the impact of autonomic neurapraxia on bladder function in female rats, as well as to assess the effects of corticosteroid therapy on the recovery of bladder function in this model.



DESIGN, SETTING, AND PARTICIPANTS: Female Sprague-Dawley rats were subjected to bilateral pelvic nerve crush (PNC) and perioperatively treated with betamethasone or vehicle. Bladder function and morphology of bladder tissue were evaluated and compared with sham-operated rats.



OUTCOME MEASUREMENTS AND STATISTICAL ANALYSIS: Western blot, immunohistochemistry, organ bath experiments, and cystometry.



RESULTS AND LIMITATIONS: Sham-operated rats exhibited regular micturitions without nonvoiding contractions (NVCs). Crush of all nerve branches of the pelvic plexus or PNC resulted in overflow incontinence and/or NVCs. Betamethasone treatment improved recovery of regular micturitions (87.5% compared with 27% for vehicle; p<0.05), reduced lowest bladder pressure (8±2cm H(2)O compared with 21±5cm H(2)O for vehicle; p<0.05), and reduced the amplitude of NVCs but had no effect on NVC frequency in PNC rats. Compared with vehicle, betamethasone-treated PNC rats had less CD68 (a macrophage marker) in the pelvic plexus and bladder tissue. Isolated bladder from betamethasone-treated PNC rats exhibited better nerve-induced contractions, contained more cholinergic and sensory nerves, and expressed lower amounts of collagen III than bladder tissue from vehicle-treated rats.



CONCLUSIONS: PNC causes autonomic neurapraxia and functional and morphologic changes of isolated bladder tissue that can be recorded as bladder dysfunction during awake cystometry in female rats. Perioperative systemic betamethasone treatment reduced macrophage contents of the pelvic plexus and bladder, partially counteracted changes in the bladder tissue, and had protective effects on micturition function. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
European Urology
volume
62
issue
6
pages
1076 - 1085
publisher
Elsevier
external identifiers
  • WOS:000310783500031
  • PMID:22542670
  • Scopus:84868558800
ISSN
1873-7560
DOI
10.1016/j.eururo.2012.04.037
language
English
LU publication?
yes
id
8bf20344-fc74-446e-ab10-c924cde81f31 (old id 2609389)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/22542670?dopt=Abstract
date added to LUP
2012-06-01 11:28:29
date last changed
2016-11-13 04:28:32
@misc{8bf20344-fc74-446e-ab10-c924cde81f31,
  abstract     = {BACKGROUND: Information on autonomic neurapraxia in female urogenital surgery is scarce, and a model to study it is not available. OBJECTIVE: To develop a model to study the impact of autonomic neurapraxia on bladder function in female rats, as well as to assess the effects of corticosteroid therapy on the recovery of bladder function in this model. <br/><br>
<br/><br>
DESIGN, SETTING, AND PARTICIPANTS: Female Sprague-Dawley rats were subjected to bilateral pelvic nerve crush (PNC) and perioperatively treated with betamethasone or vehicle. Bladder function and morphology of bladder tissue were evaluated and compared with sham-operated rats. <br/><br>
<br/><br>
OUTCOME MEASUREMENTS AND STATISTICAL ANALYSIS: Western blot, immunohistochemistry, organ bath experiments, and cystometry. <br/><br>
<br/><br>
RESULTS AND LIMITATIONS: Sham-operated rats exhibited regular micturitions without nonvoiding contractions (NVCs). Crush of all nerve branches of the pelvic plexus or PNC resulted in overflow incontinence and/or NVCs. Betamethasone treatment improved recovery of regular micturitions (87.5% compared with 27% for vehicle; p&lt;0.05), reduced lowest bladder pressure (8±2cm H(2)O compared with 21±5cm H(2)O for vehicle; p&lt;0.05), and reduced the amplitude of NVCs but had no effect on NVC frequency in PNC rats. Compared with vehicle, betamethasone-treated PNC rats had less CD68 (a macrophage marker) in the pelvic plexus and bladder tissue. Isolated bladder from betamethasone-treated PNC rats exhibited better nerve-induced contractions, contained more cholinergic and sensory nerves, and expressed lower amounts of collagen III than bladder tissue from vehicle-treated rats. <br/><br>
<br/><br>
CONCLUSIONS: PNC causes autonomic neurapraxia and functional and morphologic changes of isolated bladder tissue that can be recorded as bladder dysfunction during awake cystometry in female rats. Perioperative systemic betamethasone treatment reduced macrophage contents of the pelvic plexus and bladder, partially counteracted changes in the bladder tissue, and had protective effects on micturition function.},
  author       = {Castiglione, Fabio and Bergamini, Alice and Bettiga, Arianna and Bivalacqua, Trinity J and Benigni, Fabio and Strittmatter, Frank and Gandaglia, Giorgio and Rigatti, Patrizio and Montorsi, Francesco and Hedlund, Petter},
  issn         = {1873-7560},
  language     = {eng},
  number       = {6},
  pages        = {1076--1085},
  publisher    = {ARRAY(0x7b306f8)},
  series       = {European Urology},
  title        = {Perioperative Betamethasone Treatment Reduces Signs of Bladder Dysfunction in a Rat Model for Neurapraxia in Female Urogenital Surgery.},
  url          = {http://dx.doi.org/10.1016/j.eururo.2012.04.037},
  volume       = {62},
  year         = {2012},
}