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A Microcalorimetric Study of Resting and Activated Cellular Systems

Gebreselassie, Daniel (1996)
Abstract
Several cell types and nerve tissue have been studied using a combination of microcalorimetry and other methods.



The heat production rate and its pH dependence of normal and transformed 3T3 cells were studied. The transformed cells manifested a higher heat production rate and a larger anaerobic contribution to the total catabolism than did the non-transformed cells. Moreover, the transformed cell line was characterized by a more pH-dependent metabolism than was the normal parental cell line.



Human lymphocytes were characterized thermochemically. A heat production rate of 1 pW per cell was observed. This calorimetric value was in agreement with the value calculated from analytical data. Lymphocytes... (More)
Several cell types and nerve tissue have been studied using a combination of microcalorimetry and other methods.



The heat production rate and its pH dependence of normal and transformed 3T3 cells were studied. The transformed cells manifested a higher heat production rate and a larger anaerobic contribution to the total catabolism than did the non-transformed cells. Moreover, the transformed cell line was characterized by a more pH-dependent metabolism than was the normal parental cell line.



Human lymphocytes were characterized thermochemically. A heat production rate of 1 pW per cell was observed. This calorimetric value was in agreement with the value calculated from analytical data. Lymphocytes stimulated with phytohaemagglutinin showed an immediate increase in thermal power, a two-fold increase in lactate production and a decrease in oxygen consumption.



The behaviour of rat peritoneal macrophages under normal conditions, experimental acute liver injury (ALI) and acute renal failure (RF) were studied by use of microcalorimetry and luminometry. The two techniques gave different results for the ALI and RF groups, indicating differences in the mechanisms by which these two conditions affect the macrophage system.



The effects of endotoxin and Lactobacillus pretreatments in acute liver injury induced by D-galactosamine were studied. The results may explain why pretreatment with endotoxin is beneficial in acute liver injury induced by D-galactosamine, but not with Gram positive bacteria including Lactobacillus.



Microcalorimetry was used to study the thermal power from the regenerating rat vagus nerve continuously for several days. The truncated insulin-like growth factor (IGF-1) increased the thermal power whereas it was decreased by vinblastine. The results suggest the fraction of the thermal power related to axonal transport or to proliferation of Schwann cells. (Less)
Please use this url to cite or link to this publication:
author
opponent
  • Dr Gustafsson, Lena, Göteborg University
publishing date
type
Thesis
publication status
published
subject
keywords
Insulin like growth factors, Axoplasmic transport, Endotoxin, Lactobacillus, D-Galactosamine, Phorbol 12-myristate-13-acetate, Peritoneal macrophages, Luminometer, Phytohaemagglutinin, Lymphocytes, Microcalorimetry, 3T3 cells, Nerve regeneration, Chemistry, Kemi
pages
70 pages
publisher
Thermochemistry, P O Box 124, S-221 00 Lund,
defense location
Lecture hall C, Chemical Center
defense date
1996-03-01 13:15
external identifiers
  • Other:ISRN: LUNDD/NKKT--96/1001--SE
language
English
LU publication?
no
id
3b9af03a-6bb3-4ced-b54a-f3020a6e42ab (old id 28217)
date added to LUP
2007-06-08 15:58:50
date last changed
2016-09-19 08:45:04
@misc{3b9af03a-6bb3-4ced-b54a-f3020a6e42ab,
  abstract     = {Several cell types and nerve tissue have been studied using a combination of microcalorimetry and other methods.<br/><br>
<br/><br>
The heat production rate and its pH dependence of normal and transformed 3T3 cells were studied. The transformed cells manifested a higher heat production rate and a larger anaerobic contribution to the total catabolism than did the non-transformed cells. Moreover, the transformed cell line was characterized by a more pH-dependent metabolism than was the normal parental cell line.<br/><br>
<br/><br>
Human lymphocytes were characterized thermochemically. A heat production rate of 1 pW per cell was observed. This calorimetric value was in agreement with the value calculated from analytical data. Lymphocytes stimulated with phytohaemagglutinin showed an immediate increase in thermal power, a two-fold increase in lactate production and a decrease in oxygen consumption.<br/><br>
<br/><br>
The behaviour of rat peritoneal macrophages under normal conditions, experimental acute liver injury (ALI) and acute renal failure (RF) were studied by use of microcalorimetry and luminometry. The two techniques gave different results for the ALI and RF groups, indicating differences in the mechanisms by which these two conditions affect the macrophage system.<br/><br>
<br/><br>
The effects of endotoxin and Lactobacillus pretreatments in acute liver injury induced by D-galactosamine were studied. The results may explain why pretreatment with endotoxin is beneficial in acute liver injury induced by D-galactosamine, but not with Gram positive bacteria including Lactobacillus.<br/><br>
<br/><br>
Microcalorimetry was used to study the thermal power from the regenerating rat vagus nerve continuously for several days. The truncated insulin-like growth factor (IGF-1) increased the thermal power whereas it was decreased by vinblastine. The results suggest the fraction of the thermal power related to axonal transport or to proliferation of Schwann cells.},
  author       = {Gebreselassie, Daniel},
  keyword      = {Insulin like growth factors,Axoplasmic transport,Endotoxin,Lactobacillus,D-Galactosamine,Phorbol 12-myristate-13-acetate,Peritoneal macrophages,Luminometer,Phytohaemagglutinin,Lymphocytes,Microcalorimetry,3T3 cells,Nerve regeneration,Chemistry,Kemi},
  language     = {eng},
  pages        = {70},
  publisher    = {ARRAY(0xb8d1958)},
  title        = {A Microcalorimetric Study of Resting and Activated Cellular Systems},
  year         = {1996},
}