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Differential gene expression in adipose tissue from obese human subjects during weight loss and weight maintenance.

Johansson, Lovisa; Danielsson, Anders LU ; Parikh, Hemang; Klintenberg, Maria LU ; Norström, Fredrik LU ; Groop, Leif LU and Ridderstråle, Martin LU (2012) In The American journal of clinical nutrition 96(1). p.196-207
Abstract
BACKGROUND:

Differential gene expression in adipose tissue during diet-induced weight loss followed by a weight stability period is poorly characterized. Markers of these processes may provide a deeper understanding of underlying mechanisms.



OBJECTIVE:

We aimed to identify differentially expressed genes in human adipose tissue during weight loss and weight maintenance after weight loss.



DESIGN:

RNA from subcutaneous abdominal adipose tissue from 9 obese subjects was analyzed by using a complementary DNA microarray at baseline after weight loss on a low-calorie diet and after weight maintenance.



RESULTS:

Subjects lost 18.8 ± 1.8% of weight and... (More)
BACKGROUND:

Differential gene expression in adipose tissue during diet-induced weight loss followed by a weight stability period is poorly characterized. Markers of these processes may provide a deeper understanding of underlying mechanisms.



OBJECTIVE:

We aimed to identify differentially expressed genes in human adipose tissue during weight loss and weight maintenance after weight loss.



DESIGN:

RNA from subcutaneous abdominal adipose tissue from 9 obese subjects was analyzed by using a complementary DNA microarray at baseline after weight loss on a low-calorie diet and after weight maintenance.



RESULTS:

Subjects lost 18.8 ± 1.8% of weight and maintained this loss during weight maintenance (1.1 ± 2.1%; range: -9.3 to 10.6%). Most differentially expressed genes exhibited a reciprocal regulation and returned to baseline after weight loss (2163 genes) and weight maintenance (3175 genes). CETP and ABCG1, both of which participate in the HDL-mediated reverse cholesterol transport (RCT), were among the most upregulated of the 750 genes that were differentially expressed after both processes. Several genes involved in inflammation were downregulated. The use of real-time polymerase chain reaction confirmed or partially confirmed the previously implicated genes TNMD and MMP9 (both downregulated), PNPLA3 (upregulated), and CIDEA and SCD (both reciprocally regulated).



CONCLUSIONS:

The beneficial effects of weight loss should be investigated after long-term weight maintenance. The processes of weight loss and weight maintenance should be viewed as biologically distinct. CETP and ABCG1 may be important mediators of these effects through HDL-mediated RCT. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
The American journal of clinical nutrition
volume
96
issue
1
pages
196 - 207
publisher
American Society for Clinical Nutrition
external identifiers
  • WOS:000305670100025
  • PMID:22648723
  • Scopus:84863562798
ISSN
1938-3207
DOI
10.3945/ajcn.111.020578
language
English
LU publication?
yes
id
e008ee2d-6e0b-4a58-973a-efac952f84dd (old id 2859938)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/22648723?dopt=Abstract
date added to LUP
2012-07-04 11:21:40
date last changed
2016-12-04 04:40:12
@misc{e008ee2d-6e0b-4a58-973a-efac952f84dd,
  abstract     = {BACKGROUND:<br/><br>
Differential gene expression in adipose tissue during diet-induced weight loss followed by a weight stability period is poorly characterized. Markers of these processes may provide a deeper understanding of underlying mechanisms.<br/><br>
<br/><br>
OBJECTIVE:<br/><br>
We aimed to identify differentially expressed genes in human adipose tissue during weight loss and weight maintenance after weight loss.<br/><br>
<br/><br>
DESIGN:<br/><br>
RNA from subcutaneous abdominal adipose tissue from 9 obese subjects was analyzed by using a complementary DNA microarray at baseline after weight loss on a low-calorie diet and after weight maintenance.<br/><br>
<br/><br>
RESULTS:<br/><br>
Subjects lost 18.8 ± 1.8% of weight and maintained this loss during weight maintenance (1.1 ± 2.1%; range: -9.3 to 10.6%). Most differentially expressed genes exhibited a reciprocal regulation and returned to baseline after weight loss (2163 genes) and weight maintenance (3175 genes). CETP and ABCG1, both of which participate in the HDL-mediated reverse cholesterol transport (RCT), were among the most upregulated of the 750 genes that were differentially expressed after both processes. Several genes involved in inflammation were downregulated. The use of real-time polymerase chain reaction confirmed or partially confirmed the previously implicated genes TNMD and MMP9 (both downregulated), PNPLA3 (upregulated), and CIDEA and SCD (both reciprocally regulated).<br/><br>
<br/><br>
CONCLUSIONS:<br/><br>
The beneficial effects of weight loss should be investigated after long-term weight maintenance. The processes of weight loss and weight maintenance should be viewed as biologically distinct. CETP and ABCG1 may be important mediators of these effects through HDL-mediated RCT.},
  author       = {Johansson, Lovisa and Danielsson, Anders and Parikh, Hemang and Klintenberg, Maria and Norström, Fredrik and Groop, Leif and Ridderstråle, Martin},
  issn         = {1938-3207},
  language     = {eng},
  number       = {1},
  pages        = {196--207},
  publisher    = {ARRAY(0x9f87778)},
  series       = {The American journal of clinical nutrition},
  title        = {Differential gene expression in adipose tissue from obese human subjects during weight loss and weight maintenance.},
  url          = {http://dx.doi.org/10.3945/ajcn.111.020578},
  volume       = {96},
  year         = {2012},
}