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MHC class I-induced apoptosis in hematopoietic cells

Wallén Öhman, Marie (1996)
Abstract
The aims of this study have been focused on the identification of cell surface structures involved in the induction and regulation of apoptosis. Monoclonal antibodies against the major histocompatibility complex class I (MHC-I) were shown to induce apoptosis when cross-linked on the cell surface of different malignant cells having a pre-B or a myeloid precursor phenotype. The apoptotic response was not epitope dependent, since several different anti-MHC-I antibodies, reacting with different monomorphic determinants of MHC-I all induced apoptosis in these cells. However, external cross-linking of antibodies was strictly required for the apoptotic effect. Among cells originating from mature peripheral blood lymphocytes, anti-CD40 stimulated... (More)
The aims of this study have been focused on the identification of cell surface structures involved in the induction and regulation of apoptosis. Monoclonal antibodies against the major histocompatibility complex class I (MHC-I) were shown to induce apoptosis when cross-linked on the cell surface of different malignant cells having a pre-B or a myeloid precursor phenotype. The apoptotic response was not epitope dependent, since several different anti-MHC-I antibodies, reacting with different monomorphic determinants of MHC-I all induced apoptosis in these cells. However, external cross-linking of antibodies was strictly required for the apoptotic effect. Among cells originating from mature peripheral blood lymphocytes, anti-CD40 stimulated B-cells were susceptible to anti-MHC-I induced apoptosis, whereas resting as well as superantigen activated B- and T-cells were non-responsive to MHC-I ligation. The apoptotic process was not linked to protein kinase C activation or changes in cytoplasmic calcium concentration. In situ terminal deoxynucleotidyl transferase staining of apoptotic cells at various stages during MHC-I induced cell death revealed that apoptosis occurred predominantly in the G2/M phase of the cell cycle, with the first apoptotic cells appearing after approximately 12 hours of incubation. The results suggest a role for MHC-I mediated apoptosis during differentiation and activation of certain hematopoietic cells. (Less)
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author
opponent
  • Prof. Jondal, Mikael, Stockholm
publishing date
type
Thesis
publication status
published
subject
keywords
serology, Immunology, Bioteknik, Biotechnology, antibody, CD40, Myeloid cells, B-cells, HLA class I, cell death, transplantation, Immunologi, serologi
pages
50 pages
publisher
Marie Wallén Öhman, Sotarevägen 10, S-227 30 Lund, Sweden
defense location
Blå Hallen, Ekologihuset, Lund University
defense date
1996-09-28 10:15
external identifiers
  • Other:ISRN: LUTKDH/TKIT--96/1004--SE
ISBN
91-628-2162-8
language
English
LU publication?
no
id
da271bd7-f5f3-4b03-9dc8-0e702c3352ed (old id 28691)
date added to LUP
2007-06-13 16:12:38
date last changed
2016-09-19 08:45:06
@misc{da271bd7-f5f3-4b03-9dc8-0e702c3352ed,
  abstract     = {The aims of this study have been focused on the identification of cell surface structures involved in the induction and regulation of apoptosis. Monoclonal antibodies against the major histocompatibility complex class I (MHC-I) were shown to induce apoptosis when cross-linked on the cell surface of different malignant cells having a pre-B or a myeloid precursor phenotype. The apoptotic response was not epitope dependent, since several different anti-MHC-I antibodies, reacting with different monomorphic determinants of MHC-I all induced apoptosis in these cells. However, external cross-linking of antibodies was strictly required for the apoptotic effect. Among cells originating from mature peripheral blood lymphocytes, anti-CD40 stimulated B-cells were susceptible to anti-MHC-I induced apoptosis, whereas resting as well as superantigen activated B- and T-cells were non-responsive to MHC-I ligation. The apoptotic process was not linked to protein kinase C activation or changes in cytoplasmic calcium concentration. In situ terminal deoxynucleotidyl transferase staining of apoptotic cells at various stages during MHC-I induced cell death revealed that apoptosis occurred predominantly in the G2/M phase of the cell cycle, with the first apoptotic cells appearing after approximately 12 hours of incubation. The results suggest a role for MHC-I mediated apoptosis during differentiation and activation of certain hematopoietic cells.},
  author       = {Wallén Öhman, Marie},
  isbn         = {91-628-2162-8},
  keyword      = {serology,Immunology,Bioteknik,Biotechnology,antibody,CD40,Myeloid cells,B-cells,HLA class I,cell death,transplantation,Immunologi,serologi},
  language     = {eng},
  pages        = {50},
  publisher    = {ARRAY(0xc166b40)},
  title        = {MHC class I-induced apoptosis in hematopoietic cells},
  year         = {1996},
}