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Tendon healing in vivo : An experimental model

Abrahamsson, Sven Olof LU ; Lundborg, G. LU and Lohmander, Stefan LU (1989) In Scandinavian Journal of Plastic and Reconstructive Surgery and Hand Surgery 23(3). p.199-205
Abstract

Flexor tendon segments were incubated in a diffusion chamber in the subcutis of rabbits. Tendons incubated up to 6 weeks in the diffusion chamber showed proliferating and migrating cells from the epitenon cell layer as well as viable endotenon cells. Explants frozen in liquid nitrogen prior to incubation showed no signs of extrinsic cell contamination and remained non-viable indicating that no cell penetration occurred through the Millipore filter and that cell division seen in non-frozen and incubated tendons was an expression of intrinsic cellular proliferative capacity of the tendon. In tendon segments incubated in chambers for three weeks, collagen synthesis was reduced by 50% and the rate of cell proliferation measured as... (More)

Flexor tendon segments were incubated in a diffusion chamber in the subcutis of rabbits. Tendons incubated up to 6 weeks in the diffusion chamber showed proliferating and migrating cells from the epitenon cell layer as well as viable endotenon cells. Explants frozen in liquid nitrogen prior to incubation showed no signs of extrinsic cell contamination and remained non-viable indicating that no cell penetration occurred through the Millipore filter and that cell division seen in non-frozen and incubated tendons was an expression of intrinsic cellular proliferative capacity of the tendon. In tendon segments incubated in chambers for three weeks, collagen synthesis was reduced by 50% and the rate of cell proliferation measured as 3H-thymidine incorporation, was 15 times that of native tendons. Frozen and incubated tendons showed only traces of remaining matrix synthesis or cell proliferation. With this experimental model we have histologically and biochemically shown that tendons may survive and heal while the nutrition exclusively could be based on diffusion and the tendons have an intrinsic capacity of healing. The described model enables further studies on tendon healing and its regulation. © 1989 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.

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Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Cell proliferation, Chamber, Collagen, Tendon healing
in
Scandinavian Journal of Plastic and Reconstructive Surgery and Hand Surgery
volume
23
issue
3
pages
7 pages
publisher
Taylor & Francis
external identifiers
  • Scopus:0024784216
ISSN
0284-4311
DOI
10.3109/02844318909075118
language
English
LU publication?
yes
id
2dab7428-caad-4353-8750-1ec4011f5021
date added to LUP
2016-05-04 18:11:35
date last changed
2016-10-13 05:07:51
@misc{2dab7428-caad-4353-8750-1ec4011f5021,
  abstract     = {<p>Flexor tendon segments were incubated in a diffusion chamber in the subcutis of rabbits. Tendons incubated up to 6 weeks in the diffusion chamber showed proliferating and migrating cells from the epitenon cell layer as well as viable endotenon cells. Explants frozen in liquid nitrogen prior to incubation showed no signs of extrinsic cell contamination and remained non-viable indicating that no cell penetration occurred through the Millipore filter and that cell division seen in non-frozen and incubated tendons was an expression of intrinsic cellular proliferative capacity of the tendon. In tendon segments incubated in chambers for three weeks, collagen synthesis was reduced by 50% and the rate of cell proliferation measured as <sup>3</sup>H-thymidine incorporation, was 15 times that of native tendons. Frozen and incubated tendons showed only traces of remaining matrix synthesis or cell proliferation. With this experimental model we have histologically and biochemically shown that tendons may survive and heal while the nutrition exclusively could be based on diffusion and the tendons have an intrinsic capacity of healing. The described model enables further studies on tendon healing and its regulation. © 1989 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.</p>},
  author       = {Abrahamsson, Sven Olof and Lundborg, G. and Lohmander, Stefan},
  issn         = {0284-4311},
  keyword      = {Cell proliferation,Chamber,Collagen,Tendon healing},
  language     = {eng},
  number       = {3},
  pages        = {199--205},
  publisher    = {ARRAY(0x8062c28)},
  series       = {Scandinavian Journal of Plastic and Reconstructive Surgery and Hand Surgery},
  title        = {Tendon healing in vivo : An experimental model},
  url          = {http://dx.doi.org/10.3109/02844318909075118},
  volume       = {23},
  year         = {1989},
}