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Functionally involved cell surface antigens on murine lymphokine activated killer cells

Belfrage, Hans LU ; Bhiladvala, Pallonji LU and Kalland, T (1987) In Immunology letters 14(4). p.8-293
Abstract
To investigate the possible involvement of some cell surface structures on lymphoid cells in the functional activity of lymphokine activated killer (LAK) cells, a number of monoclonal antibodies (Mab) against such structures was studied for their ability to inhibit LAK activity in a standard cytotoxicity assay against the natural killer-insensitive target cell EL-4. Almost complete inhibition of LAK activity resulted from incubation with antibodies to the LFA-1 antigen, while blocking of the Lyt 2 antigen reduced cytotoxic activity about 50%. Mab to T-200 gave a weak and inconsistent inhibitory activity, while antibodies to Thy 1, L3T4, IL-2 receptor and MHC class I antigens were without effect. Mab to LFA-1 and Lyt 2 inhibited LAK... (More)
To investigate the possible involvement of some cell surface structures on lymphoid cells in the functional activity of lymphokine activated killer (LAK) cells, a number of monoclonal antibodies (Mab) against such structures was studied for their ability to inhibit LAK activity in a standard cytotoxicity assay against the natural killer-insensitive target cell EL-4. Almost complete inhibition of LAK activity resulted from incubation with antibodies to the LFA-1 antigen, while blocking of the Lyt 2 antigen reduced cytotoxic activity about 50%. Mab to T-200 gave a weak and inconsistent inhibitory activity, while antibodies to Thy 1, L3T4, IL-2 receptor and MHC class I antigens were without effect. Mab to LFA-1 and Lyt 2 inhibited LAK activity towards EL-4, YAC-1 and differentiated F-9 teratocarcinoma cells, but did not affect LAK-mediated killing of undifferentiated F-9 cells. Experiments with separate preincubation of effector and target cells revealed that both LFA-1 and Lyt 2 inhibited LAK activity at the effector cell level only. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Immunology letters
volume
14
issue
4
pages
8 - 293
publisher
Elsevier
external identifiers
  • Scopus:0023121380
language
English
LU publication?
yes
id
f658e841-f727-4eb8-96ac-2495d551918e (old id 32308)
date added to LUP
2007-06-19 14:29:58
date last changed
2016-10-13 04:43:53
@misc{f658e841-f727-4eb8-96ac-2495d551918e,
  abstract     = {To investigate the possible involvement of some cell surface structures on lymphoid cells in the functional activity of lymphokine activated killer (LAK) cells, a number of monoclonal antibodies (Mab) against such structures was studied for their ability to inhibit LAK activity in a standard cytotoxicity assay against the natural killer-insensitive target cell EL-4. Almost complete inhibition of LAK activity resulted from incubation with antibodies to the LFA-1 antigen, while blocking of the Lyt 2 antigen reduced cytotoxic activity about 50%. Mab to T-200 gave a weak and inconsistent inhibitory activity, while antibodies to Thy 1, L3T4, IL-2 receptor and MHC class I antigens were without effect. Mab to LFA-1 and Lyt 2 inhibited LAK activity towards EL-4, YAC-1 and differentiated F-9 teratocarcinoma cells, but did not affect LAK-mediated killing of undifferentiated F-9 cells. Experiments with separate preincubation of effector and target cells revealed that both LFA-1 and Lyt 2 inhibited LAK activity at the effector cell level only.},
  author       = {Belfrage, Hans and Bhiladvala, Pallonji and Kalland, T},
  language     = {eng},
  number       = {4},
  pages        = {8--293},
  publisher    = {ARRAY(0x8a8eda0)},
  series       = {Immunology letters},
  title        = {Functionally involved cell surface antigens on murine lymphokine activated killer cells},
  volume       = {14},
  year         = {1987},
}