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Turnover of proteoglycans in guinea pig costal cartilage

Lohmander, Stefan LU (1977) In Archives of Biochemistry and Biophysics 180(1). p.93-101
Abstract

The turnover in vivo of proteoglycans of guinea pig costal cartilage was investigated using Na2
35SO4 as precursor. Proteoglycans were extracted with guanidine · HCl, at both low and high ionic strength, and purified and fractionated by ultracentrifugation in CsCl gradients under associative and dissociative conditions. The results suggest that the sulfate is incorporated into macromolecules of at least two major metabolic pools with half-lives of about 3 days and about 60-70 days, respectively. Molecules with a fast turnover were enriched in the low ionic strength extracts and in fractions containing small, nonaggregated proteoglycans. No substantial evidence was found for a precursor-product... (More)

The turnover in vivo of proteoglycans of guinea pig costal cartilage was investigated using Na2
35SO4 as precursor. Proteoglycans were extracted with guanidine · HCl, at both low and high ionic strength, and purified and fractionated by ultracentrifugation in CsCl gradients under associative and dissociative conditions. The results suggest that the sulfate is incorporated into macromolecules of at least two major metabolic pools with half-lives of about 3 days and about 60-70 days, respectively. Molecules with a fast turnover were enriched in the low ionic strength extracts and in fractions containing small, nonaggregated proteoglycans. No substantial evidence was found for a precursor-product relationship between different fractions. © 1977.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Archives of Biochemistry and Biophysics
volume
180
issue
1
pages
9 pages
publisher
Academic Press
external identifiers
  • Scopus:0017325819
ISSN
0003-9861
DOI
10.1016/0003-9861(77)90012-1
language
English
LU publication?
yes
id
35d6157e-c4c3-4c80-954c-7813feaa7c08
date added to LUP
2016-05-04 12:44:54
date last changed
2016-11-14 09:42:50
@misc{35d6157e-c4c3-4c80-954c-7813feaa7c08,
  abstract     = {<p>The turnover in vivo of proteoglycans of guinea pig costal cartilage was investigated using Na<sub>2</sub><br>
<sup>35</sup>SO<sub>4</sub> as precursor. Proteoglycans were extracted with guanidine · HCl, at both low and high ionic strength, and purified and fractionated by ultracentrifugation in CsCl gradients under associative and dissociative conditions. The results suggest that the sulfate is incorporated into macromolecules of at least two major metabolic pools with half-lives of about 3 days and about 60-70 days, respectively. Molecules with a fast turnover were enriched in the low ionic strength extracts and in fractions containing small, nonaggregated proteoglycans. No substantial evidence was found for a precursor-product relationship between different fractions. © 1977.</p>},
  author       = {Lohmander, Stefan},
  issn         = {0003-9861},
  language     = {eng},
  month        = {04},
  number       = {1},
  pages        = {93--101},
  publisher    = {ARRAY(0xaf4f718)},
  series       = {Archives of Biochemistry and Biophysics},
  title        = {Turnover of proteoglycans in guinea pig costal cartilage},
  url          = {http://dx.doi.org/10.1016/0003-9861(77)90012-1},
  volume       = {180},
  year         = {1977},
}