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The functional anatomy of kappa promoters

Bemark, Mats LU (1998)
Abstract
Immunoglobulin kappa promoters were collected from mice and man. It was shown that they had homology within but not between subgroups, and the promoters were also conserved between species. The octamer element was found in all promoters, but other sequence elements were conserved within the distinct subgroups. E-boxes were found in most promoters and were usually of the E2A type. A mouse kappa promoter was studied functionally. Two elements were identified 5' of the octamer, the pd and the k-Y element. Both had weak stimulatory activity per se, but interacted with the octamer and each other to direct high levels of transcription. The two ets proteins PU.1 and elf-1 were shown to bind to the k-Y element. Two independent protein binding... (More)
Immunoglobulin kappa promoters were collected from mice and man. It was shown that they had homology within but not between subgroups, and the promoters were also conserved between species. The octamer element was found in all promoters, but other sequence elements were conserved within the distinct subgroups. E-boxes were found in most promoters and were usually of the E2A type. A mouse kappa promoter was studied functionally. Two elements were identified 5' of the octamer, the pd and the k-Y element. Both had weak stimulatory activity per se, but interacted with the octamer and each other to direct high levels of transcription. The two ets proteins PU.1 and elf-1 were shown to bind to the k-Y element. Two independent protein binding sites were found in the pd element, and both were needed for function of the element. One of the pd interacting proteins was identified as CArG box binding factor-A (CBF-A) after purification and amino acid sequencing. The protein interacted with both single- and double-stranded DNA, and interacted with elf-1 and PU.1 in pull down assays. A novel k-Y binding protein, Spi-C, was identified in an yeast one-hybrid screen. The protein was closely related to PU.1 within the ets domain but not outside of it. Spi-C was highly expressed in peripheral B cells while neither in pre-B cell lines nor plasmacytomas. No expression was found in a panel of different cell lines and tissues that did not represent B cells, except for weak expression in a macrophage cell line. (Less)
Please use this url to cite or link to this publication:
author
opponent
  • Dr Scheidereit, C., Berlin
organization
publishing date
type
Thesis
publication status
published
subject
keywords
histochemistry, cytochemistry, Histology, transcription, octamer, promoters, tissue culture, Histologi, cytokemi, histokemi, vävnadskultur
pages
70 pages
publisher
Department of Immunology, Lund University
defense location
Lecture Hall at Solvegatan 33
defense date
1998-05-11 10:15
external identifiers
  • Other:ISRN: LUMEDW/MECM--98/1012--SE
ISBN
91-628-2958-0
language
English
LU publication?
yes
id
1532e998-5001-4037-aaaf-e4a42c61ac71 (old id 38601)
date added to LUP
2007-06-20 11:09:37
date last changed
2016-09-19 08:45:14
@misc{1532e998-5001-4037-aaaf-e4a42c61ac71,
  abstract     = {Immunoglobulin kappa promoters were collected from mice and man. It was shown that they had homology within but not between subgroups, and the promoters were also conserved between species. The octamer element was found in all promoters, but other sequence elements were conserved within the distinct subgroups. E-boxes were found in most promoters and were usually of the E2A type. A mouse kappa promoter was studied functionally. Two elements were identified 5' of the octamer, the pd and the k-Y element. Both had weak stimulatory activity per se, but interacted with the octamer and each other to direct high levels of transcription. The two ets proteins PU.1 and elf-1 were shown to bind to the k-Y element. Two independent protein binding sites were found in the pd element, and both were needed for function of the element. One of the pd interacting proteins was identified as CArG box binding factor-A (CBF-A) after purification and amino acid sequencing. The protein interacted with both single- and double-stranded DNA, and interacted with elf-1 and PU.1 in pull down assays. A novel k-Y binding protein, Spi-C, was identified in an yeast one-hybrid screen. The protein was closely related to PU.1 within the ets domain but not outside of it. Spi-C was highly expressed in peripheral B cells while neither in pre-B cell lines nor plasmacytomas. No expression was found in a panel of different cell lines and tissues that did not represent B cells, except for weak expression in a macrophage cell line.},
  author       = {Bemark, Mats},
  isbn         = {91-628-2958-0},
  keyword      = {histochemistry,cytochemistry,Histology,transcription,octamer,promoters,tissue culture,Histologi,cytokemi,histokemi,vävnadskultur},
  language     = {eng},
  pages        = {70},
  publisher    = {ARRAY(0xa168e18)},
  title        = {The functional anatomy of kappa promoters},
  year         = {1998},
}