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Experimental Neuroretinal Transplantation

Ghosh, Fredrik LU (1999)
Abstract
Embryonic full-thickness rabbit neuroretinal sheets were transplanted to the subretinal space of adult hosts. This was accomplished by using a new transplantation technique involving vitrectomy and retinotomy. The grafts were followed from 10 to 306 days after surgery, and were then examined by different histological techniques. In the light microscope, the transplants were seen to develop the normal retinal lamination and fused with the host retina, especially after long survival times. Ultrastructurally, normal photoreceptor outer segments, well integrated with the host retinal pigment epithelium were found. Growth cones were present in the zone of fusion between graft and host retina. Immunohistochemical labeling revealed many of the... (More)
Embryonic full-thickness rabbit neuroretinal sheets were transplanted to the subretinal space of adult hosts. This was accomplished by using a new transplantation technique involving vitrectomy and retinotomy. The grafts were followed from 10 to 306 days after surgery, and were then examined by different histological techniques. In the light microscope, the transplants were seen to develop the normal retinal lamination and fused with the host retina, especially after long survival times. Ultrastructurally, normal photoreceptor outer segments, well integrated with the host retinal pigment epithelium were found. Growth cones were present in the zone of fusion between graft and host retina. Immunohistochemical labeling revealed many of the normal retinal components not previously found in retinal transplants, and graft-host connections between neurons in the rod pathway were seen. The morphology of vibratome sectioned neuroretinal sheets as well as adult full-thickness grafts was also examined. These transplantation types showed less of the normal morphology compared with embryonic full-thickness grafts. The immunogenicity of embryonic full-thickness and fragmented grafts was compared using Major Histocompatibility Complex (MHC) immunolabeling. Fragmented grafts elicited a response from the host immune system similar to a chronic transplant rejection. This reaction was absent in the full-thickness grafts which is in accordance with their good long-term survival. (Less)
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author
opponent
  • Professor Lund, Raymond, London, ENGLAND
organization
publishing date
type
Thesis
publication status
published
subject
keywords
Transplant, Retinal degeneration, Retina, Rabbit, Neural development, Immunohistochemistry, MHC, Ultrastructure, Vitrectomy, Ophtalmology, Oftalmologi
pages
143 pages
publisher
Department of Ophthalmology, Lund University
defense location
Lund
defense date
1999-03-05 10:15
external identifiers
  • Other:ISRN: LUMEDW/MEVL--1019--SE
  • Scopus:0032831559
ISBN
91-628-3330-8
language
English
LU publication?
yes
id
c6a9b356-5707-4a1a-b075-8cf3526ebcc2 (old id 39304)
date added to LUP
2007-06-20 16:26:38
date last changed
2016-09-19 08:45:07
@misc{c6a9b356-5707-4a1a-b075-8cf3526ebcc2,
  abstract     = {Embryonic full-thickness rabbit neuroretinal sheets were transplanted to the subretinal space of adult hosts. This was accomplished by using a new transplantation technique involving vitrectomy and retinotomy. The grafts were followed from 10 to 306 days after surgery, and were then examined by different histological techniques. In the light microscope, the transplants were seen to develop the normal retinal lamination and fused with the host retina, especially after long survival times. Ultrastructurally, normal photoreceptor outer segments, well integrated with the host retinal pigment epithelium were found. Growth cones were present in the zone of fusion between graft and host retina. Immunohistochemical labeling revealed many of the normal retinal components not previously found in retinal transplants, and graft-host connections between neurons in the rod pathway were seen. The morphology of vibratome sectioned neuroretinal sheets as well as adult full-thickness grafts was also examined. These transplantation types showed less of the normal morphology compared with embryonic full-thickness grafts. The immunogenicity of embryonic full-thickness and fragmented grafts was compared using Major Histocompatibility Complex (MHC) immunolabeling. Fragmented grafts elicited a response from the host immune system similar to a chronic transplant rejection. This reaction was absent in the full-thickness grafts which is in accordance with their good long-term survival.},
  author       = {Ghosh, Fredrik},
  isbn         = {91-628-3330-8},
  keyword      = {Transplant,Retinal degeneration,Retina,Rabbit,Neural development,Immunohistochemistry,MHC,Ultrastructure,Vitrectomy,Ophtalmology,Oftalmologi},
  language     = {eng},
  pages        = {143},
  publisher    = {ARRAY(0x90db950)},
  title        = {Experimental Neuroretinal Transplantation},
  year         = {1999},
}