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Chemical characterization of cyanogen bromide fragments from the beta-chain of human complement factor C3

Lundwall, Åke LU ; Hellman, U.; Eggertsen, G. and Sjöquist, J. (1984) In FEBS Lett 169(1). p.57-62
Abstract
The isolated beta-chain of human complement factor C3 (C3 beta) was fragmented by cyanogen bromide. Nine fragments were defined by gel filtration and high-pressure liquid chromatography, and characterized with respect to their Mr, amino acid composition and N-terminal amino acid sequence. Approx. 30% of the primary structure of C3 beta was determined. Alignment of the 3 N-terminal fragments allowed determination of 61 of the amino terminal residues of C3 beta. This region demonstrated 40% homology with the sequence in the N-terminal segment of the alpha-chain of the cobra venom factor.
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author
publishing date
type
Contribution to journal
publication status
published
subject
keywords
*Cyanogen Bromide, Complement C3/*analysis, High Pressure Liquid, Gel, Chromatography, Amino Acid Sequence, Amino Acids/analysis, Humans, Peptide Fragments/*analysis, Research Support, Non-U.S. Gov't
in
FEBS Lett
volume
169
issue
1
pages
57 - 62
publisher
Wiley-Blackwell
language
English
LU publication?
no
id
90cc740a-84d1-4434-9e04-4a140bfa42b7 (old id 3965056)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=6714418
date added to LUP
2013-08-11 14:58:35
date last changed
2016-11-17 15:32:49
@misc{90cc740a-84d1-4434-9e04-4a140bfa42b7,
  abstract     = {The isolated beta-chain of human complement factor C3 (C3 beta) was fragmented by cyanogen bromide. Nine fragments were defined by gel filtration and high-pressure liquid chromatography, and characterized with respect to their Mr, amino acid composition and N-terminal amino acid sequence. Approx. 30% of the primary structure of C3 beta was determined. Alignment of the 3 N-terminal fragments allowed determination of 61 of the amino terminal residues of C3 beta. This region demonstrated 40% homology with the sequence in the N-terminal segment of the alpha-chain of the cobra venom factor.},
  author       = {Lundwall, Åke and Hellman, U. and Eggertsen, G. and Sjöquist, J.},
  keyword      = {*Cyanogen Bromide,Complement C3/*analysis,High Pressure Liquid,Gel,Chromatography,Amino Acid Sequence,Amino Acids/analysis,Humans,Peptide Fragments/*analysis,Research Support,Non-U.S. Gov't},
  language     = {eng},
  number       = {1},
  pages        = {57--62},
  publisher    = {ARRAY(0x96d4970)},
  series       = {FEBS Lett},
  title        = {Chemical characterization of cyanogen bromide fragments from the beta-chain of human complement factor C3},
  volume       = {169},
  year         = {1984},
}