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A cDNA coding for human sex hormone binding globulin. Homology to vitamin K-dependent protein S

Gershagen, S. ; Fernlund, Per LU and Lundwall, Åke LU (1987) In FEBS Letters 220(1). p.35-129
Abstract
Affinity purified antibodies to human sex hormone binding globulin (SHBG) were used in screening a human liver cDNA library, constructed in the expression vector lambda gt11. One clone, identified as producing recombinant SHBG, carried a cDNA insert of 1.1 kb. The nucleotide sequence of the insert had an open reading frame coding for 356 amino acid residues. The coding sequence was followed by a short 3'-region of 19 non-translated nucleotides and a poly(A) tail. Confirmation that the cDNA clone represented human SHBG was obtained by the finding of a complete agreement in amino acid sequence with several peptide fragments generated from purified SHBG by proteolytic cleavage. The primary structure of SHBG shows a considerable homology to... (More)
Affinity purified antibodies to human sex hormone binding globulin (SHBG) were used in screening a human liver cDNA library, constructed in the expression vector lambda gt11. One clone, identified as producing recombinant SHBG, carried a cDNA insert of 1.1 kb. The nucleotide sequence of the insert had an open reading frame coding for 356 amino acid residues. The coding sequence was followed by a short 3'-region of 19 non-translated nucleotides and a poly(A) tail. Confirmation that the cDNA clone represented human SHBG was obtained by the finding of a complete agreement in amino acid sequence with several peptide fragments generated from purified SHBG by proteolytic cleavage. The primary structure of SHBG shows a considerable homology to that of protein S, a vitamin K-dependent protein with functions in the coagulation system. (Less)
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author
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publication status
published
subject
keywords
Protein S, Peptide Fragments/analysis, Liver/analysis, Humans, Glycoproteins/analysis/*genetics, DNA/*analysis, Comparative Study, Cattle, *Base Sequence, Amino Acid Sequence, Animals, Research Support, Non-U.S. Gov't, *Sequence Homology, Nucleic Acid, Sex Hormone-Binding Globulin/analysis/*genetics
in
FEBS Letters
volume
220
issue
1
pages
35 - 129
publisher
Wiley-Blackwell
external identifiers
  • scopus:0023273737
ISSN
1873-3468
language
English
LU publication?
yes
additional info
1
id
e85083db-805b-4366-bba0-07c67b230da7 (old id 3965145)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=2956126
date added to LUP
2016-04-04 13:50:12
date last changed
2021-08-29 05:33:01
@article{e85083db-805b-4366-bba0-07c67b230da7,
  abstract     = {{Affinity purified antibodies to human sex hormone binding globulin (SHBG) were used in screening a human liver cDNA library, constructed in the expression vector lambda gt11. One clone, identified as producing recombinant SHBG, carried a cDNA insert of 1.1 kb. The nucleotide sequence of the insert had an open reading frame coding for 356 amino acid residues. The coding sequence was followed by a short 3'-region of 19 non-translated nucleotides and a poly(A) tail. Confirmation that the cDNA clone represented human SHBG was obtained by the finding of a complete agreement in amino acid sequence with several peptide fragments generated from purified SHBG by proteolytic cleavage. The primary structure of SHBG shows a considerable homology to that of protein S, a vitamin K-dependent protein with functions in the coagulation system.}},
  author       = {{Gershagen, S. and Fernlund, Per and Lundwall, Åke}},
  issn         = {{1873-3468}},
  keywords     = {{Protein S; Peptide Fragments/analysis; Liver/analysis; Humans; Glycoproteins/analysis/*genetics; DNA/*analysis; Comparative Study; Cattle; *Base Sequence; Amino Acid Sequence; Animals; Research Support; Non-U.S. Gov't; *Sequence Homology; Nucleic Acid; Sex Hormone-Binding Globulin/analysis/*genetics}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{35--129}},
  publisher    = {{Wiley-Blackwell}},
  series       = {{FEBS Letters}},
  title        = {{A cDNA coding for human sex hormone binding globulin. Homology to vitamin K-dependent protein S}},
  url          = {{http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=2956126}},
  volume       = {{220}},
  year         = {{1987}},
}