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Activation of the zymogen form of prostate-specific antigen by human glandular kallikrein 2

Lövgren, Janita LU ; Rajakoski, K.; Karp, M.; Lundwall, Åke LU and Lilja, Hans LU (1997) In Biochem Biophys Res Commun 238(2). p.55-549
Abstract
Prostate-specific antigen (PSA) and human glandular kallikrein 2 (hK2) are glandular kallikreins secreted by the prostate gland. Both enzymes are synthesized with a propeptide that is supposedly cleaved off in the prostate to yield the mature forms found in semen. We have purified and characterised recombinant PSA and hK2 produced in eucaryotic cells. Recombinant PSA was recovered as a zymogen and recombinant hK2 was recovered in mature form. The zymogen form of PSA had no or very low enzymatic activity. After incubation with hK2, proPSA was activated, as shown by the cleavage of the seminal gel proteins and a peptide substrate; the hK2-proPSA ratio used was similar to the enzyme-substrate ratio that prevails under phyciological... (More)
Prostate-specific antigen (PSA) and human glandular kallikrein 2 (hK2) are glandular kallikreins secreted by the prostate gland. Both enzymes are synthesized with a propeptide that is supposedly cleaved off in the prostate to yield the mature forms found in semen. We have purified and characterised recombinant PSA and hK2 produced in eucaryotic cells. Recombinant PSA was recovered as a zymogen and recombinant hK2 was recovered in mature form. The zymogen form of PSA had no or very low enzymatic activity. After incubation with hK2, proPSA was activated, as shown by the cleavage of the seminal gel proteins and a peptide substrate; the hK2-proPSA ratio used was similar to the enzyme-substrate ratio that prevails under phyciological conditions. Our results indicate that hK2 is responsible for the activation of proPSA, a finding that may be very important for understanding of the role of these two kallikreins in the reproductive system and in prostate cancer biology. (Less)
Please use this url to cite or link to this publication:
author
organization
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type
Contribution to journal
publication status
published
subject
keywords
Research Support, Recombinant Proteins/metabolism, Prostate-Specific Antigen/*metabolism, Kallikreins/*metabolism, Humans, Enzyme Activation, Enzyme Precursors/*metabolism, Non-U.S. Gov't, Substrate Specificity, Tissue Kallikreins
in
Biochem Biophys Res Commun
volume
238
issue
2
pages
55 - 549
publisher
Elsevier
external identifiers
  • Scopus:0031577554
language
English
LU publication?
yes
id
028e6009-ab92-4456-bc4f-70226d8f24a7 (old id 3965266)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=9299549
date added to LUP
2013-08-11 14:21:12
date last changed
2016-10-13 04:55:03
@misc{028e6009-ab92-4456-bc4f-70226d8f24a7,
  abstract     = {Prostate-specific antigen (PSA) and human glandular kallikrein 2 (hK2) are glandular kallikreins secreted by the prostate gland. Both enzymes are synthesized with a propeptide that is supposedly cleaved off in the prostate to yield the mature forms found in semen. We have purified and characterised recombinant PSA and hK2 produced in eucaryotic cells. Recombinant PSA was recovered as a zymogen and recombinant hK2 was recovered in mature form. The zymogen form of PSA had no or very low enzymatic activity. After incubation with hK2, proPSA was activated, as shown by the cleavage of the seminal gel proteins and a peptide substrate; the hK2-proPSA ratio used was similar to the enzyme-substrate ratio that prevails under phyciological conditions. Our results indicate that hK2 is responsible for the activation of proPSA, a finding that may be very important for understanding of the role of these two kallikreins in the reproductive system and in prostate cancer biology.},
  author       = {Lövgren, Janita and Rajakoski, K. and Karp, M. and Lundwall, Åke and Lilja, Hans},
  keyword      = {Research Support,Recombinant Proteins/metabolism,Prostate-Specific Antigen/*metabolism,Kallikreins/*metabolism,Humans,Enzyme Activation,Enzyme Precursors/*metabolism,Non-U.S. Gov't,Substrate Specificity,Tissue Kallikreins},
  language     = {eng},
  number       = {2},
  pages        = {55--549},
  publisher    = {ARRAY(0x92389e8)},
  series       = {Biochem Biophys Res Commun},
  title        = {Activation of the zymogen form of prostate-specific antigen by human glandular kallikrein 2},
  volume       = {238},
  year         = {1997},
}