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Isolation and characterization of primary bone marrow mesenchymal stromal cells

Li, Hongzhe LU ; Ghazanfari, Roshanak LU ; Zacharaki, Dimitra LU ; Lim, Hooi Ching LU and Scheding, Stefan LU (2016) In Annals of the New York Academy of Sciences 1370(1). p.109-118
Abstract

Bone marrow (BM) contains a rare population of mesenchymal stromal cells (MSCs), which have been characterized as nonhematopoietic skeletal progenitor cells with central importance for the hematopoietic microenvironment. Classically, MSCs are isolated by plastic adherence and subsequent culture. However, as cultured stromal cells differ from their in vivo progenitors, it is important to identify the phenotype of the primary MSCs to study these cells in more detail. In the past years, several surface markers have been reported to be suitable for effective enrichment of BM-MSCs, and recent data indicate that the putative MSC stem/progenitor cell population in human adult BM is highly enriched in Lin CD45... (More)

Bone marrow (BM) contains a rare population of mesenchymal stromal cells (MSCs), which have been characterized as nonhematopoietic skeletal progenitor cells with central importance for the hematopoietic microenvironment. Classically, MSCs are isolated by plastic adherence and subsequent culture. However, as cultured stromal cells differ from their in vivo progenitors, it is important to identify the phenotype of the primary MSCs to study these cells in more detail. In the past years, several surface markers have been reported to be suitable for effective enrichment of BM-MSCs, and recent data indicate that the putative MSC stem/progenitor cell population in human adult BM is highly enriched in Lin CD45 CD271+ CD140a (PDGFRα)low/− cells. Moreover, surface marker combinations have been described for the isolation of MSCs from murine BM. On the basis of these findings, the role of primary MSCs can now be studied in normal and, importantly, diseased BM. Furthermore, genetically engineered mouse models have been developed as powerful tools to investigate well-defined BM stromal cell populations in vivo. Our discussion aims to provide a concise overview of the current state of the art in BM-MSC isolation in humans and briefly present murine MSC isolation approaches and genetic models.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
bone marrow, bone marrow stromal cells, CD271, CFU-F, mesenchymal stromal cells, MSC, PDGFRα
in
Annals of the New York Academy of Sciences
volume
1370
issue
1
pages
10 pages
publisher
New York Academy of Sciences
external identifiers
  • Scopus:84976641469
ISSN
0077-8923
DOI
10.1111/nyas.13102
language
English
LU publication?
yes
id
3f3d65e4-c9ac-4489-89e3-9a1edcbc5d1b
date added to LUP
2016-07-18 13:32:55
date last changed
2016-10-13 05:11:46
@misc{3f3d65e4-c9ac-4489-89e3-9a1edcbc5d1b,
  abstract     = {<p>Bone marrow (BM) contains a rare population of mesenchymal stromal cells (MSCs), which have been characterized as nonhematopoietic skeletal progenitor cells with central importance for the hematopoietic microenvironment. Classically, MSCs are isolated by plastic adherence and subsequent culture. However, as cultured stromal cells differ from their in vivo progenitors, it is important to identify the phenotype of the primary MSCs to study these cells in more detail. In the past years, several surface markers have been reported to be suitable for effective enrichment of BM-MSCs, and recent data indicate that the putative MSC stem/progenitor cell population in human adult BM is highly enriched in Lin<sup>−</sup> CD45<sup>−</sup> CD271<sup>+</sup> CD140a (PDGFRα)<sup>low/−</sup> cells. Moreover, surface marker combinations have been described for the isolation of MSCs from murine BM. On the basis of these findings, the role of primary MSCs can now be studied in normal and, importantly, diseased BM. Furthermore, genetically engineered mouse models have been developed as powerful tools to investigate well-defined BM stromal cell populations in vivo. Our discussion aims to provide a concise overview of the current state of the art in BM-MSC isolation in humans and briefly present murine MSC isolation approaches and genetic models.</p>},
  author       = {Li, Hongzhe and Ghazanfari, Roshanak and Zacharaki, Dimitra and Lim, Hooi Ching and Scheding, Stefan},
  issn         = {0077-8923},
  keyword      = {bone marrow,bone marrow stromal cells,CD271,CFU-F,mesenchymal stromal cells,MSC,PDGFRα},
  language     = {eng},
  month        = {04},
  number       = {1},
  pages        = {109--118},
  publisher    = {ARRAY(0x9b46b98)},
  series       = {Annals of the New York Academy of Sciences},
  title        = {Isolation and characterization of primary bone marrow mesenchymal stromal cells},
  url          = {http://dx.doi.org/10.1111/nyas.13102},
  volume       = {1370},
  year         = {2016},
}