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Engineering of thermometric biosensors and characterisation of redox enzymes for improved performance of biosensors/biofuel cells

Yakovleva, Maria LU (2013)
Abstract
The present work is mainly focused on the development of enzyme-based bioanalytical devices, which can further be applied as analytical tools for industrial, medical and environmental applications.

The first part of the work deals with the practical applications of thermometric biosensors for determination of various sugars (fructose and lactose) in a real samples. Thermometric biosensors possess properties, which make them appealing compared with for instance the widely utilised amperometric biosensors for application in certain areas as discussed in a review. Demonstration of the capabilities of a ‘hybrid’ biosensor technology combining the advantages of two different detection principles is also discussed.

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The present work is mainly focused on the development of enzyme-based bioanalytical devices, which can further be applied as analytical tools for industrial, medical and environmental applications.

The first part of the work deals with the practical applications of thermometric biosensors for determination of various sugars (fructose and lactose) in a real samples. Thermometric biosensors possess properties, which make them appealing compared with for instance the widely utilised amperometric biosensors for application in certain areas as discussed in a review. Demonstration of the capabilities of a ‘hybrid’ biosensor technology combining the advantages of two different detection principles is also discussed.

Another direction of the work in focused on a comprehensive characterisation of a promising redox enzyme – pyranose dehydrogenase (PDH) for further application in the development of amperometric biosensors and fabrication of bioanodes. The electrocatalytical performance of the enzyme is studied using two different electron transfer mechanisms: i.e., mediated electron transfer, by wiring PDH with osmium-bound redox polymers on the surface of a graphite electrode, and direct electron transfer, by direct adsorption of the enzyme on the surface of a graphite electrode. The current density response obtained from oxidation of various sugars by PDH, is measured using electrochemical techniques, such as flow-injection amperometry and cyclic voltammetry. The work is aimed at improving the current output by downsizing the enzyme’s dimensions through depletion of the glycosylation, additional fragmentation and recombinant production of the mutant enzyme possessing a different degree of glycosylation. (Less)
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author
supervisor
opponent
  • Prof. Dr. Wollenberger, Ulla, Institute of Biochemistry and Biology, University of Potsdam, Golm, Germany
organization
publishing date
type
Thesis
publication status
published
subject
keywords
Flow injection analysis, thermometric/calorimetric biosensor, enzyme thermistor, immobilised enzyme reactor, amperometric biosensor, enzymatic biofuel cell, bioanode, pyranose dehydrogenase, cellobiose dehydrogenase, direct electron transfer, mediated electron transfer, matrix-assisted laser desorption/ionization, glycosite mapping
pages
84 pages
publisher
Department of Chemistry, Lund University
defense location
Lecture Hall B, Kemicentrum, Getingevägen 60, Lund, Sweden
defense date
2013-10-11 10:30
ISBN
978-91-7422-330-9
language
English
LU publication?
yes
id
644c1be0-97cb-4393-aa1e-85465721db19 (old id 4025503)
date added to LUP
2013-09-19 09:59:47
date last changed
2016-09-19 08:45:05
@misc{644c1be0-97cb-4393-aa1e-85465721db19,
  abstract     = {The present work is mainly focused on the development of enzyme-based bioanalytical devices, which can further be applied as analytical tools for industrial, medical and environmental applications.<br/><br>
The first part of the work deals with the practical applications of thermometric biosensors for determination of various sugars (fructose and lactose) in a real samples. Thermometric biosensors possess properties, which make them appealing compared with for instance the widely utilised amperometric biosensors for application in certain areas as discussed in a review. Demonstration of the capabilities of a ‘hybrid’ biosensor technology combining the advantages of two different detection principles is also discussed.<br/><br>
Another direction of the work in focused on a comprehensive characterisation of a promising redox enzyme – pyranose dehydrogenase (PDH) for further application in the development of amperometric biosensors and fabrication of bioanodes. The electrocatalytical performance of the enzyme is studied using two different electron transfer mechanisms: i.e., mediated electron transfer, by wiring PDH with osmium-bound redox polymers on the surface of a graphite electrode, and direct electron transfer, by direct adsorption of the enzyme on the surface of a graphite electrode. The current density response obtained from oxidation of various sugars by PDH, is measured using electrochemical techniques, such as flow-injection amperometry and cyclic voltammetry. The work is aimed at improving the current output by downsizing the enzyme’s dimensions through depletion of the glycosylation, additional fragmentation and recombinant production of the mutant enzyme possessing a different degree of glycosylation.},
  author       = {Yakovleva, Maria},
  isbn         = {978-91-7422-330-9},
  keyword      = {Flow injection analysis,thermometric/calorimetric biosensor,enzyme thermistor,immobilised enzyme reactor,amperometric biosensor,enzymatic biofuel cell,bioanode,pyranose dehydrogenase,cellobiose dehydrogenase,direct electron transfer,mediated electron transfer,matrix-assisted laser desorption/ionization,glycosite mapping},
  language     = {eng},
  pages        = {84},
  publisher    = {ARRAY(0x9d5b970)},
  title        = {Engineering of thermometric biosensors and characterisation of redox enzymes for improved performance of biosensors/biofuel cells},
  year         = {2013},
}