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Sublethal in vitro glucose-oxygen deprivation protects cultured hippocampal neurons against a subsequent severe insult

Khaspekov, Leonid; Shamloo, Mehrdad LU ; Victorov, Ilya and Wieloch, Tadeusz LU (1998) In NeuroReport 9(7). p.1273-1276
Abstract

Rat and gerbil hippocampus exposed to a sublethal period of ischemia becomes resistant to a subsequent period of lethal ischemia induced several days later, a phenomenon referred to as ischemic preconditioning. Here we describe ischemic preconditioning induced in vitro in cultured hippocampal neurons. Mixed neuroglial hippocampal cell cultures of 14-17 DIV were exposed to a combined glucose and oxygen deprivation (GOD). Cultures subjected to 90 min, but not 60 min, of GOD showed extensive degeneration after a 1 day recovery period. An episode of 60 min of preconditioning GOD followed 1 and 2 days later by 90 min of GOD resulted in 40-60% protection. The data demonstrate that ischemic preconditioning can be mimicked in an in vitro... (More)

Rat and gerbil hippocampus exposed to a sublethal period of ischemia becomes resistant to a subsequent period of lethal ischemia induced several days later, a phenomenon referred to as ischemic preconditioning. Here we describe ischemic preconditioning induced in vitro in cultured hippocampal neurons. Mixed neuroglial hippocampal cell cultures of 14-17 DIV were exposed to a combined glucose and oxygen deprivation (GOD). Cultures subjected to 90 min, but not 60 min, of GOD showed extensive degeneration after a 1 day recovery period. An episode of 60 min of preconditioning GOD followed 1 and 2 days later by 90 min of GOD resulted in 40-60% protection. The data demonstrate that ischemic preconditioning can be mimicked in an in vitro hippocampal cell culture system.

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author
organization
publishing date
type
Contribution to journal
publication status
published
keywords
Cell culture, Hippocampus, Ischemia, Neuronal death, Tolerance
in
NeuroReport
volume
9
issue
7
pages
1273 - 1276
publisher
Lippincott Williams & Wilkins
external identifiers
  • Scopus:0032507599
ISSN
0959-4965
language
English
LU publication?
yes
id
4051f7d3-970a-4882-a022-f91876a23863
date added to LUP
2016-10-05 16:08:12
date last changed
2016-11-29 09:42:12
@misc{4051f7d3-970a-4882-a022-f91876a23863,
  abstract     = {<p>Rat and gerbil hippocampus exposed to a sublethal period of ischemia becomes resistant to a subsequent period of lethal ischemia induced several days later, a phenomenon referred to as ischemic preconditioning. Here we describe ischemic preconditioning induced in vitro in cultured hippocampal neurons. Mixed neuroglial hippocampal cell cultures of 14-17 DIV were exposed to a combined glucose and oxygen deprivation (GOD). Cultures subjected to 90 min, but not 60 min, of GOD showed extensive degeneration after a 1 day recovery period. An episode of 60 min of preconditioning GOD followed 1 and 2 days later by 90 min of GOD resulted in 40-60% protection. The data demonstrate that ischemic preconditioning can be mimicked in an in vitro hippocampal cell culture system.</p>},
  author       = {Khaspekov, Leonid and Shamloo, Mehrdad and Victorov, Ilya and Wieloch, Tadeusz},
  issn         = {0959-4965},
  keyword      = {Cell culture,Hippocampus,Ischemia,Neuronal death,Tolerance},
  language     = {eng},
  month        = {05},
  number       = {7},
  pages        = {1273--1276},
  publisher    = {ARRAY(0x9ae0ce0)},
  series       = {NeuroReport},
  title        = {Sublethal in vitro glucose-oxygen deprivation protects cultured hippocampal neurons against a subsequent severe insult},
  volume       = {9},
  year         = {1998},
}