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Electron microscopic demonstration of proteoglycans in guinea pig epiphyseal cartilage

Thyberg, Johan; Lohmander, Stefan LU and Friberg, Ulf (1973) In Journal of Ultrasructure Research 45(5-6). p.407-427
Abstract

Guinea pig epiphyseal cartilage was studied ultrastructurally after staining with ruthenium red or Alcian Blue. Extracellularly, the matrix granules were positively stained with both dyes, whereas no apparent staining of the collagen fibrils occurred. Intracellularly, a positive ruthenium red staining of the Golgi vacuole granules was observed. No Alcian Blue staining of the content of the vacuoles was detected. The fresh, untreated cartilage contained about 1.3% hexosamine on a dry weight basis. About three-fourths thereof represented chondroitin sulfate, the remaining part keratan sulfate and glycoproteins. After digestion of thin cartilage slices with hyaluronidase or chondroitinase ABC the chondroitin sulfate content was less than... (More)

Guinea pig epiphyseal cartilage was studied ultrastructurally after staining with ruthenium red or Alcian Blue. Extracellularly, the matrix granules were positively stained with both dyes, whereas no apparent staining of the collagen fibrils occurred. Intracellularly, a positive ruthenium red staining of the Golgi vacuole granules was observed. No Alcian Blue staining of the content of the vacuoles was detected. The fresh, untreated cartilage contained about 1.3% hexosamine on a dry weight basis. About three-fourths thereof represented chondroitin sulfate, the remaining part keratan sulfate and glycoproteins. After digestion of thin cartilage slices with hyaluronidase or chondroitinase ABC the chondroitin sulfate content was less than 50% of that in the buffer-incubated controls. Concomitantly, the matrix granules were reduced in size and number or completely absent. Papain digestion removed more than 90% of the chondroitin sulfate and all matrix granules. From these findings it was concluded that the matrix granules contain proteoglycans. Moreover, it seemed reasonable to assume that the Golgi vacuole granules, at least in part, represent intracellular proteoglycans. Signs of secretion of these granules by exocytosis were occasionally observed. © 1973 Academic Press, Inc.

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type
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publication status
published
subject
in
Journal of Ultrasructure Research
volume
45
issue
5-6
pages
21 pages
publisher
Academic Press Inc.
external identifiers
  • Scopus:0015729996
ISSN
0022-5320
DOI
10.1016/S0022-5320(73)80070-X
language
English
LU publication?
yes
id
408ae4fb-d22a-45cf-a6d4-af41f1052d06
date added to LUP
2016-05-04 12:37:11
date last changed
2016-11-27 04:39:59
@misc{408ae4fb-d22a-45cf-a6d4-af41f1052d06,
  abstract     = {<p>Guinea pig epiphyseal cartilage was studied ultrastructurally after staining with ruthenium red or Alcian Blue. Extracellularly, the matrix granules were positively stained with both dyes, whereas no apparent staining of the collagen fibrils occurred. Intracellularly, a positive ruthenium red staining of the Golgi vacuole granules was observed. No Alcian Blue staining of the content of the vacuoles was detected. The fresh, untreated cartilage contained about 1.3% hexosamine on a dry weight basis. About three-fourths thereof represented chondroitin sulfate, the remaining part keratan sulfate and glycoproteins. After digestion of thin cartilage slices with hyaluronidase or chondroitinase ABC the chondroitin sulfate content was less than 50% of that in the buffer-incubated controls. Concomitantly, the matrix granules were reduced in size and number or completely absent. Papain digestion removed more than 90% of the chondroitin sulfate and all matrix granules. From these findings it was concluded that the matrix granules contain proteoglycans. Moreover, it seemed reasonable to assume that the Golgi vacuole granules, at least in part, represent intracellular proteoglycans. Signs of secretion of these granules by exocytosis were occasionally observed. © 1973 Academic Press, Inc.</p>},
  author       = {Thyberg, Johan and Lohmander, Stefan and Friberg, Ulf},
  issn         = {0022-5320},
  language     = {eng},
  number       = {5-6},
  pages        = {407--427},
  publisher    = {ARRAY(0x9793f98)},
  series       = {Journal of Ultrasructure Research},
  title        = {Electron microscopic demonstration of proteoglycans in guinea pig epiphyseal cartilage},
  url          = {http://dx.doi.org/10.1016/S0022-5320(73)80070-X},
  volume       = {45},
  year         = {1973},
}