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Optical Analysis of biological media - continuous wave diffuse spectroscopy

Sørensen Dam, Jan LU (2000) In Lund Reports in Atomic Physics LRAP-265.
Abstract
The main topic of this thesis is real-time quantification of relevant chromophores and light scattering elements in biological media. The presented methods and instrumentation are based on continuous wave (steady-state) optical measurements of (a) spatially-resolved diffuse reflectance from bulk media and (b) combined spatially-resolved and goniometric measurements of re-emitted light from thin samples. These two configurations address applications for non-invasive medical diagnostics (optical biopsy), and in vitro diffuse spectroscopy of turbid samples (e.g. whole blood analysis and immunoassays), respectively. The physical basis of light-tissue interaction, i.e. absorption and scattering, is discussed, as well as various theoretical... (More)
The main topic of this thesis is real-time quantification of relevant chromophores and light scattering elements in biological media. The presented methods and instrumentation are based on continuous wave (steady-state) optical measurements of (a) spatially-resolved diffuse reflectance from bulk media and (b) combined spatially-resolved and goniometric measurements of re-emitted light from thin samples. These two configurations address applications for non-invasive medical diagnostics (optical biopsy), and in vitro diffuse spectroscopy of turbid samples (e.g. whole blood analysis and immunoassays), respectively. The physical basis of light-tissue interaction, i.e. absorption and scattering, is discussed, as well as various theoretical models for light propagation in turbid biological media, e.g. Monte Carlo simulations, diffusion theory, and the adding-doubling method. The optical properties are extracted from the measurements using multivariate calibration and analysis techniques. Therefore, a general introduction to such methods is also included, e.g. principal component analysis, multiple polynomial regression, and Newton-Raphson prediction algorithms. Finally, some of the prototype instrumentation developed during the project is presented, e.g. a fiber probe system, an integrating sphere setup, and a hybrid goniometric/spatially-resolved system. (Less)
Please use this url to cite or link to this publication:
author
opponent
  • Dr Sterenborg, HJCM, Department of Radiotherapy, Academic Hospital Rotterdam
organization
publishing date
type
Thesis
publication status
published
subject
keywords
spatially resolved diffuse reflectance, in vitro diffuse spectroscopy, continuous wave techniques, Nuclear physics, multivariate data analysis., Kärnfysik, Fysik, Physics, Medicin (människa och djur), Medicine (human and vertebrates), Monte Carlo simulations, light propagation models, multiple scattering, Tissue optics, tissue optical properties, Fysicumarkivet A:2000:Sörensen Dam
in
Lund Reports in Atomic Physics
volume
LRAP-265
pages
176 pages
publisher
Department of Physics, Lund University
defense location
Hörsal B, Dept. of Physics
defense date
2000-12-15 10:15
external identifiers
  • Other:ISRN: LUTFD2(TFAF-1045)1-91 (2000)
ISBN
91-628-4546-2
language
English
LU publication?
yes
id
b4d2188c-7bd5-4adb-97a0-12225f04cc98 (old id 41154)
date added to LUP
2007-10-02 15:48:43
date last changed
2016-09-19 08:45:12
@misc{b4d2188c-7bd5-4adb-97a0-12225f04cc98,
  abstract     = {The main topic of this thesis is real-time quantification of relevant chromophores and light scattering elements in biological media. The presented methods and instrumentation are based on continuous wave (steady-state) optical measurements of (a) spatially-resolved diffuse reflectance from bulk media and (b) combined spatially-resolved and goniometric measurements of re-emitted light from thin samples. These two configurations address applications for non-invasive medical diagnostics (optical biopsy), and in vitro diffuse spectroscopy of turbid samples (e.g. whole blood analysis and immunoassays), respectively. The physical basis of light-tissue interaction, i.e. absorption and scattering, is discussed, as well as various theoretical models for light propagation in turbid biological media, e.g. Monte Carlo simulations, diffusion theory, and the adding-doubling method. The optical properties are extracted from the measurements using multivariate calibration and analysis techniques. Therefore, a general introduction to such methods is also included, e.g. principal component analysis, multiple polynomial regression, and Newton-Raphson prediction algorithms. Finally, some of the prototype instrumentation developed during the project is presented, e.g. a fiber probe system, an integrating sphere setup, and a hybrid goniometric/spatially-resolved system.},
  author       = {Sørensen Dam, Jan},
  isbn         = {91-628-4546-2},
  keyword      = {spatially resolved diffuse reflectance,in vitro diffuse spectroscopy,continuous wave techniques,Nuclear physics,multivariate data analysis.,Kärnfysik,Fysik,Physics,Medicin (människa och djur),Medicine (human and vertebrates),Monte Carlo simulations,light propagation models,multiple scattering,Tissue optics,tissue optical properties,Fysicumarkivet A:2000:Sörensen Dam},
  language     = {eng},
  pages        = {176},
  publisher    = {ARRAY(0xa225a68)},
  series       = {Lund Reports in Atomic Physics},
  title        = {Optical Analysis of biological media - continuous wave diffuse spectroscopy},
  volume       = {LRAP-265},
  year         = {2000},
}