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Glycoside hydrolases for extraction and modification of polyphenolic antioxidants

Gulshan Kazi, Zubaida LU ; Khan, Sami LU ; Kulkarni, Tejas LU ; Pozzo, Tania LU and Nordberg Karlsson, Eva LU (2013) In Advances in enzyme biotechnology p.9-21
Abstract
Antioxidants are important molecules that are widely used by humans, both as dietary supplements and as additives to different types of products. In this chapter, we review how flavonoids, a class of polyphenolic antioxidants that are often found in glycosylated forms in many natural resources, can be extracted and modified using glycoside hydrolases (GHs). Glycosylation is a fundamental enzymatic process in nature, affecting function of many types of molecules (glycans, proteins, lipids as well as other organic molecules such as the flavonoids). Possibilities to control glycosylation thus mean possibilities to control or modify the function of the molecule. For the flavonoids, glycosylation affect both the antioxidative power and... (More)
Antioxidants are important molecules that are widely used by humans, both as dietary supplements and as additives to different types of products. In this chapter, we review how flavonoids, a class of polyphenolic antioxidants that are often found in glycosylated forms in many natural resources, can be extracted and modified using glycoside hydrolases (GHs). Glycosylation is a fundamental enzymatic process in nature, affecting function of many types of molecules (glycans, proteins, lipids as well as other organic molecules such as the flavonoids). Possibilities to control glycosylation thus mean possibilities to control or modify the function of the molecule. For the flavonoids, glycosylation affect both the antioxidative power and solubility. In this chapter we overview results on in vitro deglycosylation and glycosylation of flavonoids by selected GHs. For optimal enzymatic performance, desired features include a correct specificity for the target, combined with high stability. Poor specificity towards a specific substituent is thus a major drawback for enzymes in particular applications. Efforts to develop the enzymes as conversion tools are reviewed. (Less)
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author
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
quercetin, flavonoid, GH, glycosynthase, cellulase, amylase, glucosidase
in
Advances in enzyme biotechnology
editor
Shukla, P and Pletschke, B
pages
9 - 21
publisher
Springer
ISBN
978-81-322-1093-1
language
English
LU publication?
yes
id
d1c73c86-96bf-4104-880c-583b577e859c (old id 4499478)
date added to LUP
2014-07-03 13:39:48
date last changed
2016-04-16 08:11:44
@misc{d1c73c86-96bf-4104-880c-583b577e859c,
  abstract     = {Antioxidants are important molecules that are widely used by humans, both as dietary supplements and as additives to different types of products. In this chapter, we review how flavonoids, a class of polyphenolic antioxidants that are often found in glycosylated forms in many natural resources, can be extracted and modified using glycoside hydrolases (GHs). Glycosylation is a fundamental enzymatic process in nature, affecting function of many types of molecules (glycans, proteins, lipids as well as other organic molecules such as the flavonoids). Possibilities to control glycosylation thus mean possibilities to control or modify the function of the molecule. For the flavonoids, glycosylation affect both the antioxidative power and solubility. In this chapter we overview results on in vitro deglycosylation and glycosylation of flavonoids by selected GHs. For optimal enzymatic performance, desired features include a correct specificity for the target, combined with high stability. Poor specificity towards a specific substituent is thus a major drawback for enzymes in particular applications. Efforts to develop the enzymes as conversion tools are reviewed.},
  author       = {Gulshan Kazi, Zubaida and Khan, Sami and Kulkarni, Tejas and Pozzo, Tania and Nordberg Karlsson, Eva},
  editor       = {Shukla, P and Pletschke, B},
  isbn         = {978-81-322-1093-1},
  keyword      = {quercetin,flavonoid,GH,glycosynthase,cellulase,amylase,glucosidase},
  language     = {eng},
  pages        = {9--21},
  publisher    = {ARRAY(0x11ac8a48)},
  series       = {Advances in enzyme biotechnology},
  title        = {Glycoside hydrolases for extraction and modification of polyphenolic antioxidants},
  year         = {2013},
}