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Inhibition of serum diamine oxidase discloses a constitutive putrescine release from cultured vascular smooth muscle cells

Nilsson, B O LU ; Kockum, I. and Rosengren, E (1999) In Inflammation Research 48(4). p.80-176
Abstract

OBJECTIVE: To determine if putrescine and the higher polyamines spermidine and spermine are released from cultured vascular smooth muscle cells.

MATERIAL: Vascular smooth muscle cell line A7r5.

TREATMENT: Cells were treated with aminoguanidine (10 or 100 microM) for 1 to 24 h with or without fetal calf serum (10%) present in the culture medium.

METHODS: Cellular and medium concentrations of polyamines were determined by liquid chromatography. Total cellular protein was determined by the Bradford procedure. Student's two-tailed t-test was used for statistical calculations.

RESULTS: A constitutive release of putrescine was disclosed within 5 h if serum diamine oxidase was inhibited by 10 microM aminoguanidine. The... (More)

OBJECTIVE: To determine if putrescine and the higher polyamines spermidine and spermine are released from cultured vascular smooth muscle cells.

MATERIAL: Vascular smooth muscle cell line A7r5.

TREATMENT: Cells were treated with aminoguanidine (10 or 100 microM) for 1 to 24 h with or without fetal calf serum (10%) present in the culture medium.

METHODS: Cellular and medium concentrations of polyamines were determined by liquid chromatography. Total cellular protein was determined by the Bradford procedure. Student's two-tailed t-test was used for statistical calculations.

RESULTS: A constitutive release of putrescine was disclosed within 5 h if serum diamine oxidase was inhibited by 10 microM aminoguanidine. The release was linear with time for 24 h and specific for putrescine in the sense that the higher polyamines spermidine and spermine, despite similar cellular concentrations, were not released. Similar amounts of putrescine were released from the smooth muscle cells whether or not culture medium contained serum. Cells, that had been cultured in medium from which fetal calf serum had been omitted for last 48 h, contained less putrescine, spermidine and protein than those that persisted in medium that contained serum.

CONCLUSION: A constitutive putrescine release from vascular smooth muscle cells is disclosed in the presence of aminoguanidine.

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Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Amine Oxidase (Copper-Containing), Animals, Cells, Cultured, Enzyme Inhibitors, Guanidines, Muscle, Smooth, Vascular, Putrescine, Rats
in
Inflammation Research
volume
48
issue
4
pages
5 pages
publisher
Birkhaüser
external identifiers
  • Scopus:0032907337
ISSN
1023-3830
DOI
10.1007/s000110050443
language
English
LU publication?
yes
id
474c23d9-c78e-471a-ba24-79a6a7c3dcb2
date added to LUP
2016-06-17 16:36:25
date last changed
2016-10-13 05:10:16
@misc{474c23d9-c78e-471a-ba24-79a6a7c3dcb2,
  abstract     = {<p>OBJECTIVE: To determine if putrescine and the higher polyamines spermidine and spermine are released from cultured vascular smooth muscle cells.</p><p>MATERIAL: Vascular smooth muscle cell line A7r5.</p><p>TREATMENT: Cells were treated with aminoguanidine (10 or 100 microM) for 1 to 24 h with or without fetal calf serum (10%) present in the culture medium.</p><p>METHODS: Cellular and medium concentrations of polyamines were determined by liquid chromatography. Total cellular protein was determined by the Bradford procedure. Student's two-tailed t-test was used for statistical calculations.</p><p>RESULTS: A constitutive release of putrescine was disclosed within 5 h if serum diamine oxidase was inhibited by 10 microM aminoguanidine. The release was linear with time for 24 h and specific for putrescine in the sense that the higher polyamines spermidine and spermine, despite similar cellular concentrations, were not released. Similar amounts of putrescine were released from the smooth muscle cells whether or not culture medium contained serum. Cells, that had been cultured in medium from which fetal calf serum had been omitted for last 48 h, contained less putrescine, spermidine and protein than those that persisted in medium that contained serum.</p><p>CONCLUSION: A constitutive putrescine release from vascular smooth muscle cells is disclosed in the presence of aminoguanidine.</p>},
  author       = {Nilsson, B O and Kockum, I. and Rosengren, E},
  issn         = {1023-3830},
  keyword      = {Amine Oxidase (Copper-Containing),Animals,Cells, Cultured,Enzyme Inhibitors,Guanidines,Muscle, Smooth, Vascular,Putrescine,Rats},
  language     = {eng},
  number       = {4},
  pages        = {80--176},
  publisher    = {ARRAY(0x8066590)},
  series       = {Inflammation Research},
  title        = {Inhibition of serum diamine oxidase discloses a constitutive putrescine release from cultured vascular smooth muscle cells},
  url          = {http://dx.doi.org/10.1007/s000110050443},
  volume       = {48},
  year         = {1999},
}