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PCR primers for 30 novel gene regions in the nuclear genomes of Lepidoptera

Wahlberg, Niklas LU ; Peña, Carlos; Ahola, Milla; Wheat, Christopher W. and Rota, Jadranka LU (2016) In ZooKeys 2016(596). p.129-141
Abstract

We report primer pairs for 30 new gene regions in the nuclear genomes of Lepidoptera that can be amplified using a standard PCR protocol. The new primers were tested across diverse Lepidoptera, including nonditrysians and a wide selection of ditrysians. These new gene regions give a total of 11,043 bp of DNA sequence data and they show similar variability to traditionally used nuclear gene regions in studies of Lepidoptera. We feel that a PCR-based approach still has its place in molecular systematic studies of Lepidoptera, particularly at the intrafamilial level, and our new set of primers now provides a route to generating phylogenomic datasets using traditional methods.

Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Lepidoptera, Molecular systematics, Phylogenetics, Phylogenomics
in
ZooKeys
volume
2016
issue
596
pages
13 pages
publisher
Pensoft Publishers
external identifiers
  • Scopus:84973367340
ISSN
1313-2989
DOI
10.3897/zookeys.596.8399
language
English
LU publication?
yes
id
4d9f721f-d8e5-4bcd-b9a6-2de8de0edb9e
date added to LUP
2016-09-08 12:10:46
date last changed
2016-10-31 09:49:35
@misc{4d9f721f-d8e5-4bcd-b9a6-2de8de0edb9e,
  abstract     = {<p>We report primer pairs for 30 new gene regions in the nuclear genomes of Lepidoptera that can be amplified using a standard PCR protocol. The new primers were tested across diverse Lepidoptera, including nonditrysians and a wide selection of ditrysians. These new gene regions give a total of 11,043 bp of DNA sequence data and they show similar variability to traditionally used nuclear gene regions in studies of Lepidoptera. We feel that a PCR-based approach still has its place in molecular systematic studies of Lepidoptera, particularly at the intrafamilial level, and our new set of primers now provides a route to generating phylogenomic datasets using traditional methods.</p>},
  author       = {Wahlberg, Niklas and Peña, Carlos and Ahola, Milla and Wheat, Christopher W. and Rota, Jadranka},
  issn         = {1313-2989},
  keyword      = {Lepidoptera,Molecular systematics,Phylogenetics,Phylogenomics},
  language     = {eng},
  number       = {596},
  pages        = {129--141},
  publisher    = {ARRAY(0x9e90e00)},
  series       = {ZooKeys},
  title        = {PCR primers for 30 novel gene regions in the nuclear genomes of Lepidoptera},
  url          = {http://dx.doi.org/10.3897/zookeys.596.8399},
  volume       = {2016},
  year         = {2016},
}