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Isolation, characterization, and enzymatic hydrolysis of acetyl-galactoglucomannan from spruce (Picea abies)

Stålbrand, Henrik LU ; Lundqvist, Jon LU ; Andersson, Alexandra LU ; Hägglund, Per LU ; Anderson, Lars LU ; Tjerneld, Folke LU ; Jacobs A,, A ; Teleman, A ; Dahlman, O and Palm, M , et al. (2004) 864. p.66-78
Abstract
Water-soluble hemicelluloses were extracted from spruce chips by heat-fractionation using microwave treatment. A screening of conditions (pH, temperature and residence time) was performed for the extraction of O-acetyl-galactoglucomannan (AcGGM). The yield and the average molecular weight of the extracted mannan were analysed using HPLC, size-exclusion chromatography (SEC) and mass-spectrometry. The pH during heat fractionation influenced the yield and the structure of AcGGM. The highest yield (78%) of AcGGM (average molecular weight 3800) was achieved with heat-fractionation in water at 190degrees C for 5 minutes. With 0.025% NaOH, an average molecular weight of 9500 was obtained at a yield of 30%. AcGGM molecular weight standard... (More)
Water-soluble hemicelluloses were extracted from spruce chips by heat-fractionation using microwave treatment. A screening of conditions (pH, temperature and residence time) was performed for the extraction of O-acetyl-galactoglucomannan (AcGGM). The yield and the average molecular weight of the extracted mannan were analysed using HPLC, size-exclusion chromatography (SEC) and mass-spectrometry. The pH during heat fractionation influenced the yield and the structure of AcGGM. The highest yield (78%) of AcGGM (average molecular weight 3800) was achieved with heat-fractionation in water at 190degrees C for 5 minutes. With 0.025% NaOH, an average molecular weight of 9500 was obtained at a yield of 30%. AcGGM molecular weight standard molecules were prepared using SEC. The structure of the AcGGM was determined using H-1-NMR. The enzymatic hydrolysis of AcGGM was studied using beta-mannanase and alpha-galactosidase (Less)
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organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
MICROWAVE IRRADIATION, PINUS-SILVESTRIS L, DEGRADING ENZYMES, GLUCOMANNAN, LIGNOCELLULOSE, CHROMATOGRAPHY, PRETREATMENT, HEMICELLULOSES, BETA-MANNANASE, POSITIONS
host publication
Hemicelluloses. science and technology. (ACS symposium series ; 864 )
volume
864
pages
66 - 78
publisher
The American Chemical Society (ACS)
external identifiers
  • scopus:38849099768
ISBN
0-8412-3842-1
language
English
LU publication?
yes
id
8325438f-3cdc-4bb6-bfdb-7bafdd04a68c (old id 526694)
date added to LUP
2016-04-04 10:31:45
date last changed
2023-09-20 07:01:49
@inproceedings{8325438f-3cdc-4bb6-bfdb-7bafdd04a68c,
  abstract     = {{Water-soluble hemicelluloses were extracted from spruce chips by heat-fractionation using microwave treatment. A screening of conditions (pH, temperature and residence time) was performed for the extraction of O-acetyl-galactoglucomannan (AcGGM). The yield and the average molecular weight of the extracted mannan were analysed using HPLC, size-exclusion chromatography (SEC) and mass-spectrometry. The pH during heat fractionation influenced the yield and the structure of AcGGM. The highest yield (78%) of AcGGM (average molecular weight 3800) was achieved with heat-fractionation in water at 190degrees C for 5 minutes. With 0.025% NaOH, an average molecular weight of 9500 was obtained at a yield of 30%. AcGGM molecular weight standard molecules were prepared using SEC. The structure of the AcGGM was determined using H-1-NMR. The enzymatic hydrolysis of AcGGM was studied using beta-mannanase and alpha-galactosidase}},
  author       = {{Stålbrand, Henrik and Lundqvist, Jon and Andersson, Alexandra and Hägglund, Per and Anderson, Lars and Tjerneld, Folke and Jacobs A,, A and Teleman, A and Dahlman, O and Palm, M and Zacchi, Guido}},
  booktitle    = {{Hemicelluloses. science and technology. (ACS symposium series ; 864 )}},
  isbn         = {{0-8412-3842-1}},
  keywords     = {{MICROWAVE IRRADIATION; PINUS-SILVESTRIS L; DEGRADING ENZYMES; GLUCOMANNAN; LIGNOCELLULOSE; CHROMATOGRAPHY; PRETREATMENT; HEMICELLULOSES; BETA-MANNANASE; POSITIONS}},
  language     = {{eng}},
  pages        = {{66--78}},
  publisher    = {{The American Chemical Society (ACS)}},
  title        = {{Isolation, characterization, and enzymatic hydrolysis of acetyl-galactoglucomannan from spruce (Picea abies)}},
  volume       = {{864}},
  year         = {{2004}},
}