Advanced

Isolation, characterization, and enzymatic hydrolysis of acetyl-galactoglucomannan from spruce (Picea abies)

Stålbrand, Henrik LU ; Lundqvist, Jon LU ; Andersson, Alexandra LU ; Hägglund, Per LU ; Anderson, Lars LU ; Tjerneld, Folke LU ; Jacobs A,, A; Teleman, A; Dahlman, O and Palm, M, et al. (2004) In Hemicelluloses. science and technology. (ACS symposium series ; 864 ) 864. p.66-78
Abstract
Water-soluble hemicelluloses were extracted from spruce chips by heat-fractionation using microwave treatment. A screening of conditions (pH, temperature and residence time) was performed for the extraction of O-acetyl-galactoglucomannan (AcGGM). The yield and the average molecular weight of the extracted mannan were analysed using HPLC, size-exclusion chromatography (SEC) and mass-spectrometry. The pH during heat fractionation influenced the yield and the structure of AcGGM. The highest yield (78%) of AcGGM (average molecular weight 3800) was achieved with heat-fractionation in water at 190degrees C for 5 minutes. With 0.025% NaOH, an average molecular weight of 9500 was obtained at a yield of 30%. AcGGM molecular weight standard... (More)
Water-soluble hemicelluloses were extracted from spruce chips by heat-fractionation using microwave treatment. A screening of conditions (pH, temperature and residence time) was performed for the extraction of O-acetyl-galactoglucomannan (AcGGM). The yield and the average molecular weight of the extracted mannan were analysed using HPLC, size-exclusion chromatography (SEC) and mass-spectrometry. The pH during heat fractionation influenced the yield and the structure of AcGGM. The highest yield (78%) of AcGGM (average molecular weight 3800) was achieved with heat-fractionation in water at 190degrees C for 5 minutes. With 0.025% NaOH, an average molecular weight of 9500 was obtained at a yield of 30%. AcGGM molecular weight standard molecules were prepared using SEC. The structure of the AcGGM was determined using H-1-NMR. The enzymatic hydrolysis of AcGGM was studied using beta-mannanase and alpha-galactosidase (Less)
Please use this url to cite or link to this publication:
author
, et al. (More)
(Less)
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
MICROWAVE IRRADIATION, PINUS-SILVESTRIS L, DEGRADING ENZYMES, GLUCOMANNAN, LIGNOCELLULOSE, CHROMATOGRAPHY, PRETREATMENT, HEMICELLULOSES, BETA-MANNANASE, POSITIONS
in
Hemicelluloses. science and technology. (ACS symposium series ; 864 )
volume
864
pages
66 - 78
publisher
The American Chemical Society
external identifiers
  • Scopus:38849099768
ISBN
0-8412-3842-1
language
English
LU publication?
yes
id
8325438f-3cdc-4bb6-bfdb-7bafdd04a68c (old id 526694)
date added to LUP
2007-09-25 11:36:21
date last changed
2016-10-13 04:40:31
@misc{8325438f-3cdc-4bb6-bfdb-7bafdd04a68c,
  abstract     = {Water-soluble hemicelluloses were extracted from spruce chips by heat-fractionation using microwave treatment. A screening of conditions (pH, temperature and residence time) was performed for the extraction of O-acetyl-galactoglucomannan (AcGGM). The yield and the average molecular weight of the extracted mannan were analysed using HPLC, size-exclusion chromatography (SEC) and mass-spectrometry. The pH during heat fractionation influenced the yield and the structure of AcGGM. The highest yield (78%) of AcGGM (average molecular weight 3800) was achieved with heat-fractionation in water at 190degrees C for 5 minutes. With 0.025% NaOH, an average molecular weight of 9500 was obtained at a yield of 30%. AcGGM molecular weight standard molecules were prepared using SEC. The structure of the AcGGM was determined using H-1-NMR. The enzymatic hydrolysis of AcGGM was studied using beta-mannanase and alpha-galactosidase},
  author       = {Stålbrand, Henrik and Lundqvist, Jon and Andersson, Alexandra and Hägglund, Per and Anderson, Lars and Tjerneld, Folke and Jacobs A,, A and Teleman, A and Dahlman, O and Palm, M and Zacchi, Guido},
  isbn         = {0-8412-3842-1},
  keyword      = {MICROWAVE IRRADIATION,PINUS-SILVESTRIS L,DEGRADING ENZYMES,GLUCOMANNAN,LIGNOCELLULOSE,CHROMATOGRAPHY,PRETREATMENT,HEMICELLULOSES,BETA-MANNANASE,POSITIONS},
  language     = {eng},
  pages        = {66--78},
  publisher    = {ARRAY(0x7ea0840)},
  series       = {Hemicelluloses. science and technology. (ACS symposium series ; 864 )},
  title        = {Isolation, characterization, and enzymatic hydrolysis of acetyl-galactoglucomannan from spruce (Picea abies)},
  volume       = {864},
  year         = {2004},
}