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Functional aspects of cellobiose dehydrogenase Applications for biosensor development

Stoica, Leonard LU (2005)
Abstract
Electrochemcial investigations were conducted for elucidating



and understanding the relation between the various electron transfer processes occurring in



cellobiose dehydrogenase (CDH) bound to an electrode surface. Substrate inhibition caused by



cellobiose was proven to act in competition with the electron transfer to a two-electron acceptor



and under a partial inhibition mechanism for the internal electron transfer process. The



implications of these findings have been discussed in relation to what has been stated previously



but also exploited for analytical purposes by adjusting the sensitivity of the CDH based... (More)
Electrochemcial investigations were conducted for elucidating



and understanding the relation between the various electron transfer processes occurring in



cellobiose dehydrogenase (CDH) bound to an electrode surface. Substrate inhibition caused by



cellobiose was proven to act in competition with the electron transfer to a two-electron acceptor



and under a partial inhibition mechanism for the internal electron transfer process. The



implications of these findings have been discussed in relation to what has been stated previously



but also exploited for analytical purposes by adjusting the sensitivity of the CDH based biosensor



for catecholamines.



Different thiols were used to form self-assembled monolayers (SAM) on gold electrodes and these



have been used to electrochemically investigate and characterise the direct electron transfer



reaction of CDH from two white-rot fungi (Phanerochaete sordida and Trametes villosa), and one



soft-rot fungus (Myriococcum thermophilum) in the absence and presence of cellobiose.



The high efficiency of the direct electron transfer between Phanerochaete sordida CDH and



Trametes villosa CDH and SAM modified Au electrodes was emphasised, and a potential



application of these enzymes was demonstrated by development of a third-generation biosensor for



lactose determination.



Toxic waste-water quinone type pollutants were also determined using a Phanerochaete



chrysosporium CDH based biosensor, working under a mediated electron transfer mechanism. (Less)
Please use this url to cite or link to this publication:
author
supervisor
opponent
  • Professor Scheller, Frieder W., Dept. of Analytical Biochemistry, Potsdam University, Golm, Germany
organization
publishing date
type
Thesis
publication status
published
subject
keywords
Elektrokemi, Electrochemistry, Analytisk kemi, Analytical chemistry, bioelectrochemistry, direct electron transfer, lactose biosensor
pages
184 pages
publisher
Department of Analytical Chemistry, Lund University
defense location
Centre for Chemistry and Chemical Engineering, Sölvegatan 39, Lund, Hall B
defense date
2004-03-04 10:30
ISBN
91-7422-073-X
language
English
LU publication?
yes
id
412d265b-0190-4b37-8421-574f68e404ce (old id 544363)
date added to LUP
2007-10-13 13:13:30
date last changed
2016-09-19 08:45:14
@misc{412d265b-0190-4b37-8421-574f68e404ce,
  abstract     = {Electrochemcial investigations were conducted for elucidating<br/><br>
<br/><br>
and understanding the relation between the various electron transfer processes occurring in<br/><br>
<br/><br>
cellobiose dehydrogenase (CDH) bound to an electrode surface. Substrate inhibition caused by<br/><br>
<br/><br>
cellobiose was proven to act in competition with the electron transfer to a two-electron acceptor<br/><br>
<br/><br>
and under a partial inhibition mechanism for the internal electron transfer process. The<br/><br>
<br/><br>
implications of these findings have been discussed in relation to what has been stated previously<br/><br>
<br/><br>
but also exploited for analytical purposes by adjusting the sensitivity of the CDH based biosensor<br/><br>
<br/><br>
for catecholamines.<br/><br>
<br/><br>
Different thiols were used to form self-assembled monolayers (SAM) on gold electrodes and these<br/><br>
<br/><br>
have been used to electrochemically investigate and characterise the direct electron transfer<br/><br>
<br/><br>
reaction of CDH from two white-rot fungi (Phanerochaete sordida and Trametes villosa), and one<br/><br>
<br/><br>
soft-rot fungus (Myriococcum thermophilum) in the absence and presence of cellobiose.<br/><br>
<br/><br>
The high efficiency of the direct electron transfer between Phanerochaete sordida CDH and<br/><br>
<br/><br>
Trametes villosa CDH and SAM modified Au electrodes was emphasised, and a potential<br/><br>
<br/><br>
application of these enzymes was demonstrated by development of a third-generation biosensor for<br/><br>
<br/><br>
lactose determination.<br/><br>
<br/><br>
Toxic waste-water quinone type pollutants were also determined using a Phanerochaete<br/><br>
<br/><br>
chrysosporium CDH based biosensor, working under a mediated electron transfer mechanism.},
  author       = {Stoica, Leonard},
  isbn         = {91-7422-073-X},
  keyword      = {Elektrokemi,Electrochemistry,Analytisk kemi,Analytical chemistry,bioelectrochemistry,direct electron transfer,lactose biosensor},
  language     = {eng},
  pages        = {184},
  publisher    = {ARRAY(0x7a83598)},
  title        = {Functional aspects of cellobiose dehydrogenase Applications for biosensor development},
  year         = {2005},
}