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Release of endogenous and radioactive purines from the rabbit retina

Perez, M T LU ; Ehinger, B E LU ; Lindström, K and Fredholm, B B (1986) In Brain Research 398(1). p.12-106
Abstract

The adenine nucleotide pool of rabbit retina was labeled by an intravitreal injection in vivo of [3H]adenosine. Practically all the radioactivity was retained in the form of adenine nucleotides. The relative proportion of [3H]adenine nucleotides was the same as that of endogenous nucleotides. Potassium depolarization (43.6 mM) in vitro caused a rapid increase in the rate of release of radioactive purines. The radioactive material was composed of hypoxanthine, xanthine, inosine and trace amounts of adenine, adenosine and adenine nucleotides. The release of radioactive purines was delayed and reduced by the addition of the nucleoside inhibitor dipyridamole suggesting that the purines may be released in the form of nucleosides. Similarly,... (More)

The adenine nucleotide pool of rabbit retina was labeled by an intravitreal injection in vivo of [3H]adenosine. Practically all the radioactivity was retained in the form of adenine nucleotides. The relative proportion of [3H]adenine nucleotides was the same as that of endogenous nucleotides. Potassium depolarization (43.6 mM) in vitro caused a rapid increase in the rate of release of radioactive purines. The radioactive material was composed of hypoxanthine, xanthine, inosine and trace amounts of adenine, adenosine and adenine nucleotides. The release of radioactive purines was delayed and reduced by the addition of the nucleoside inhibitor dipyridamole suggesting that the purines may be released in the form of nucleosides. Similarly, the addition of the ecto 5'-nucleotidase inhibitor alpha, beta-methylene ADP (AOPCP) did not alter the release of radioactivity or the composition of the released purines. Endogenous hypoxanthine, xanthine and inosine could be detected in the effluents, but there was only a very modest increase following potassium depolarization. There was a slight, but significant, decrease in the release of endogenous adenosine and increase in AMP after AOPCP. It is concluded that there is an intensive uptake and phosphorylation of adenosine in the rabbit retina. Depolarization induces release of radioactive purine nucleosides and bases. Most of these compounds appear to be released as such, but in addition there may be a small (maximally a few per cent of the total) fraction of the purines that are released as nucleotides.

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author
organization
publishing date
type
Contribution to journal
publication status
published
keywords
Adenine, Adenine Nucleotides, Adenosine, Adenosine Diphosphate, Animals, Dipyridamole, Evoked Potentials, Female, Hypoxanthine, Hypoxanthines, In Vitro Techniques, Male, Potassium, Purines, Rabbits, Retina, Tritium, Journal Article, Research Support, Non-U.S. Gov't
in
Brain Research
volume
398
issue
1
pages
12 - 106
publisher
Elsevier
external identifiers
  • Scopus:0023028975
ISSN
0006-8993
DOI
10.1016/0006-8993(86)91255-2
language
English
LU publication?
yes
id
5f57d654-095c-4da1-a27e-6205696ff077
date added to LUP
2016-10-27 19:36:15
date last changed
2016-11-20 04:36:47
@misc{5f57d654-095c-4da1-a27e-6205696ff077,
  abstract     = {<p>The adenine nucleotide pool of rabbit retina was labeled by an intravitreal injection in vivo of [3H]adenosine. Practically all the radioactivity was retained in the form of adenine nucleotides. The relative proportion of [3H]adenine nucleotides was the same as that of endogenous nucleotides. Potassium depolarization (43.6 mM) in vitro caused a rapid increase in the rate of release of radioactive purines. The radioactive material was composed of hypoxanthine, xanthine, inosine and trace amounts of adenine, adenosine and adenine nucleotides. The release of radioactive purines was delayed and reduced by the addition of the nucleoside inhibitor dipyridamole suggesting that the purines may be released in the form of nucleosides. Similarly, the addition of the ecto 5'-nucleotidase inhibitor alpha, beta-methylene ADP (AOPCP) did not alter the release of radioactivity or the composition of the released purines. Endogenous hypoxanthine, xanthine and inosine could be detected in the effluents, but there was only a very modest increase following potassium depolarization. There was a slight, but significant, decrease in the release of endogenous adenosine and increase in AMP after AOPCP. It is concluded that there is an intensive uptake and phosphorylation of adenosine in the rabbit retina. Depolarization induces release of radioactive purine nucleosides and bases. Most of these compounds appear to be released as such, but in addition there may be a small (maximally a few per cent of the total) fraction of the purines that are released as nucleotides.</p>},
  author       = {Perez, M T and Ehinger, B E and Lindström, K and Fredholm, B B},
  issn         = {0006-8993},
  keyword      = {Adenine,Adenine Nucleotides,Adenosine,Adenosine Diphosphate,Animals,Dipyridamole,Evoked Potentials,Female,Hypoxanthine,Hypoxanthines,In Vitro Techniques,Male,Potassium,Purines,Rabbits,Retina,Tritium,Journal Article,Research Support, Non-U.S. Gov't},
  language     = {eng},
  month        = {11},
  number       = {1},
  pages        = {12--106},
  publisher    = {ARRAY(0xa84e588)},
  series       = {Brain Research},
  title        = {Release of endogenous and radioactive purines from the rabbit retina},
  url          = {http://dx.doi.org/10.1016/0006-8993(86)91255-2},
  volume       = {398},
  year         = {1986},
}