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Growth kinetics and Pho84 phosphate transporter activity of Saccharomyces cerevisiae under phosphate-limited conditions

Shokrollahzadeh, Soheila; Bonakdarpour, Babak; Vahabzadeh, Farzaneh and Sanati, Mehri LU (2007) In Journal of Industrial Microbiology & Biotechnology 34(1). p.17-25
Abstract
The effect of phosphate (P ( i )) concentration on the growth behavior of Saccharomyces cerevisiae strain CEN.PK113-5D in phosphate-limited batch and chemostat cultures was studied. The range of dilution rates used in the present study was 0.08-0.45 h(-1). The batch growth of yeast cells followed Monod relationship, but growth of the cells in phosphate-limited chemostat showed change in growth kinetics with increasing dilution rates. The difference in growth kinetics of the yeast cells in phosphate-limited chemostat for dilution rates below and above approximately 0.2 h(-1) has been discussed in terms of the batch growth kinetic data and the change in the metabolic activity of the yeast cells. Immunological detection of a C-terminally myc... (More)
The effect of phosphate (P ( i )) concentration on the growth behavior of Saccharomyces cerevisiae strain CEN.PK113-5D in phosphate-limited batch and chemostat cultures was studied. The range of dilution rates used in the present study was 0.08-0.45 h(-1). The batch growth of yeast cells followed Monod relationship, but growth of the cells in phosphate-limited chemostat showed change in growth kinetics with increasing dilution rates. The difference in growth kinetics of the yeast cells in phosphate-limited chemostat for dilution rates below and above approximately 0.2 h(-1) has been discussed in terms of the batch growth kinetic data and the change in the metabolic activity of the yeast cells. Immunological detection of a C-terminally myc epitope-tagged Pho84 fusion protein indicated derepressive expression of the Pho84 high-affinity P ( i ) transporter in the entire range of dilution rates employed in this study. Phosphate transport activity mediated by Pho84 transporter was highest at very low dilution rates, i.e. 0.08-0.1 h(-1), corresponding to conditions in which the amount of synthesized Pho84 was at its maximum. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
phosphate, Pho84 transporter, growth kinetics, saccharomyces cerevisiae
in
Journal of Industrial Microbiology & Biotechnology
volume
34
issue
1
pages
17 - 25
publisher
Springer
external identifiers
  • Scopus:33845654425
ISSN
1367-5435
DOI
10.1007/s10295-006-0157-5
language
English
LU publication?
yes
id
9a014660-8e20-4142-958c-a7319121af32 (old id 638436)
date added to LUP
2009-02-26 11:39:35
date last changed
2016-11-18 10:41:46
@misc{9a014660-8e20-4142-958c-a7319121af32,
  abstract     = {The effect of phosphate (P ( i )) concentration on the growth behavior of Saccharomyces cerevisiae strain CEN.PK113-5D in phosphate-limited batch and chemostat cultures was studied. The range of dilution rates used in the present study was 0.08-0.45 h(-1). The batch growth of yeast cells followed Monod relationship, but growth of the cells in phosphate-limited chemostat showed change in growth kinetics with increasing dilution rates. The difference in growth kinetics of the yeast cells in phosphate-limited chemostat for dilution rates below and above approximately 0.2 h(-1) has been discussed in terms of the batch growth kinetic data and the change in the metabolic activity of the yeast cells. Immunological detection of a C-terminally myc epitope-tagged Pho84 fusion protein indicated derepressive expression of the Pho84 high-affinity P ( i ) transporter in the entire range of dilution rates employed in this study. Phosphate transport activity mediated by Pho84 transporter was highest at very low dilution rates, i.e. 0.08-0.1 h(-1), corresponding to conditions in which the amount of synthesized Pho84 was at its maximum.},
  author       = {Shokrollahzadeh, Soheila and Bonakdarpour, Babak and Vahabzadeh, Farzaneh and Sanati, Mehri},
  issn         = {1367-5435},
  keyword      = {phosphate,Pho84 transporter,growth kinetics,saccharomyces cerevisiae},
  language     = {eng},
  number       = {1},
  pages        = {17--25},
  publisher    = {ARRAY(0x9dbe4e0)},
  series       = {Journal of Industrial Microbiology & Biotechnology},
  title        = {Growth kinetics and Pho84 phosphate transporter activity of Saccharomyces cerevisiae under phosphate-limited conditions},
  url          = {http://dx.doi.org/10.1007/s10295-006-0157-5},
  volume       = {34},
  year         = {2007},
}