Growth kinetics and Pho84 phosphate transporter activity of Saccharomyces cerevisiae under phosphate-limited conditions
(2007) In Journal of Industrial Microbiology & Biotechnology 34(1). p.17-25- Abstract
- The effect of phosphate (P ( i )) concentration on the growth behavior of Saccharomyces cerevisiae strain CEN.PK113-5D in phosphate-limited batch and chemostat cultures was studied. The range of dilution rates used in the present study was 0.08-0.45 h(-1). The batch growth of yeast cells followed Monod relationship, but growth of the cells in phosphate-limited chemostat showed change in growth kinetics with increasing dilution rates. The difference in growth kinetics of the yeast cells in phosphate-limited chemostat for dilution rates below and above approximately 0.2 h(-1) has been discussed in terms of the batch growth kinetic data and the change in the metabolic activity of the yeast cells. Immunological detection of a C-terminally myc... (More)
- The effect of phosphate (P ( i )) concentration on the growth behavior of Saccharomyces cerevisiae strain CEN.PK113-5D in phosphate-limited batch and chemostat cultures was studied. The range of dilution rates used in the present study was 0.08-0.45 h(-1). The batch growth of yeast cells followed Monod relationship, but growth of the cells in phosphate-limited chemostat showed change in growth kinetics with increasing dilution rates. The difference in growth kinetics of the yeast cells in phosphate-limited chemostat for dilution rates below and above approximately 0.2 h(-1) has been discussed in terms of the batch growth kinetic data and the change in the metabolic activity of the yeast cells. Immunological detection of a C-terminally myc epitope-tagged Pho84 fusion protein indicated derepressive expression of the Pho84 high-affinity P ( i ) transporter in the entire range of dilution rates employed in this study. Phosphate transport activity mediated by Pho84 transporter was highest at very low dilution rates, i.e. 0.08-0.1 h(-1), corresponding to conditions in which the amount of synthesized Pho84 was at its maximum. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/638436
- author
- Shokrollahzadeh, Soheila ; Bonakdarpour, Babak ; Vahabzadeh, Farzaneh and Sanati, Mehri LU
- organization
- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- phosphate, Pho84 transporter, growth kinetics, saccharomyces cerevisiae
- in
- Journal of Industrial Microbiology & Biotechnology
- volume
- 34
- issue
- 1
- pages
- 17 - 25
- publisher
- Springer
- external identifiers
-
- scopus:33845654425
- ISSN
- 1367-5435
- DOI
- 10.1007/s10295-006-0157-5
- language
- English
- LU publication?
- yes
- id
- 9a014660-8e20-4142-958c-a7319121af32 (old id 638436)
- date added to LUP
- 2016-04-04 14:21:36
- date last changed
- 2022-01-30 01:50:21
@article{9a014660-8e20-4142-958c-a7319121af32,
abstract = {{The effect of phosphate (P ( i )) concentration on the growth behavior of Saccharomyces cerevisiae strain CEN.PK113-5D in phosphate-limited batch and chemostat cultures was studied. The range of dilution rates used in the present study was 0.08-0.45 h(-1). The batch growth of yeast cells followed Monod relationship, but growth of the cells in phosphate-limited chemostat showed change in growth kinetics with increasing dilution rates. The difference in growth kinetics of the yeast cells in phosphate-limited chemostat for dilution rates below and above approximately 0.2 h(-1) has been discussed in terms of the batch growth kinetic data and the change in the metabolic activity of the yeast cells. Immunological detection of a C-terminally myc epitope-tagged Pho84 fusion protein indicated derepressive expression of the Pho84 high-affinity P ( i ) transporter in the entire range of dilution rates employed in this study. Phosphate transport activity mediated by Pho84 transporter was highest at very low dilution rates, i.e. 0.08-0.1 h(-1), corresponding to conditions in which the amount of synthesized Pho84 was at its maximum.}},
author = {{Shokrollahzadeh, Soheila and Bonakdarpour, Babak and Vahabzadeh, Farzaneh and Sanati, Mehri}},
issn = {{1367-5435}},
keywords = {{phosphate; Pho84 transporter; growth kinetics; saccharomyces cerevisiae}},
language = {{eng}},
number = {{1}},
pages = {{17--25}},
publisher = {{Springer}},
series = {{Journal of Industrial Microbiology & Biotechnology}},
title = {{Growth kinetics and Pho84 phosphate transporter activity of Saccharomyces cerevisiae under phosphate-limited conditions}},
url = {{http://dx.doi.org/10.1007/s10295-006-0157-5}},
doi = {{10.1007/s10295-006-0157-5}},
volume = {{34}},
year = {{2007}},
}