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Determination of aromatic amines in aqueous extracts of polyurethane foam using hydrophilic interaction liquid chromatography and mass spectrometry

RIDDAR, JAKOB LU ; Karlsson, Daniel; Dalene, Marianne LU and Skarping, Gunnar LU (2010) In Analytica Chimica Acta 678(1). p.117-123
Abstract

A method is presented for the determination of aromatic amines in aqueous extracts of polyurethane (PUR) foam. The method is based on the extraction of PUR foam using aqueous acetic acid (0.1%, w/v) followed by determination of extracted aromatic amines using hydrophilic interaction liquid chromatography (HILIC) and tandem mass spectrometry (MS/MS) with positive electrospray ionisation. The injections of volumes up to 5 μL of aqueous solutions were made possible by on-column focusing with partially filled loop injections. The fragmentation patterns for 2,4- and 2,6-toluene diamine (TDA) and 4,4′-methylene dianiline (MDA) were clarified by performing a hydrogen-deuterium exchange study.TDA and MDA were determined using trideuterated 2,4-... (More)

A method is presented for the determination of aromatic amines in aqueous extracts of polyurethane (PUR) foam. The method is based on the extraction of PUR foam using aqueous acetic acid (0.1%, w/v) followed by determination of extracted aromatic amines using hydrophilic interaction liquid chromatography (HILIC) and tandem mass spectrometry (MS/MS) with positive electrospray ionisation. The injections of volumes up to 5 μL of aqueous solutions were made possible by on-column focusing with partially filled loop injections. The fragmentation patterns for 2,4- and 2,6-toluene diamine (TDA) and 4,4′-methylene dianiline (MDA) were clarified by performing a hydrogen-deuterium exchange study.TDA and MDA were determined using trideuterated 2,4- and 2,6-TDA and dideuterated 4,4′-MDA as internal standards. Linear calibration graphs were obtained over the range 0.025-0.5μgmL
-1 with correlation coefficients >0.996 and the instrumental detection limit for each compound was <50fmol. The stability of the amines was influenced by the matrix, so their concentrations decreased over time.Agreement was observed between the results of analyses of PUR foam extracts by HILIC-MS/MS and results obtained by ethyl chloroformate derivatisation and reversed phase (RP) liquid chromatography-mass spectrometry (LC-MS/MS).TDA was observed to be unstable in extracts of foam but not in pure solutions. © 2010 Elsevier B.V.

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author
publishing date
type
Contribution to journal
publication status
published
keywords
Hydrogen-deuterium exchange, Hydrophilic interaction liquid chromatography (HILIC), Liquid chromatography-electrospray tandem mass spectrometry, Methylene dianiline (MDA), Polyurethane (PUR) foam, Toluene diamine (TDA)
in
Analytica Chimica Acta
volume
678
issue
1
pages
7 pages
publisher
Elsevier
external identifiers
  • Scopus:77957011955
ISSN
0003-2670
DOI
10.1016/j.aca.2010.08.020
language
English
LU publication?
no
id
64fad2c1-777c-4472-9a0f-0cfdc427777e
date added to LUP
2016-10-14 10:55:07
date last changed
2016-10-14 12:11:27
@misc{64fad2c1-777c-4472-9a0f-0cfdc427777e,
  abstract     = {<p>A method is presented for the determination of aromatic amines in aqueous extracts of polyurethane (PUR) foam. The method is based on the extraction of PUR foam using aqueous acetic acid (0.1%, w/v) followed by determination of extracted aromatic amines using hydrophilic interaction liquid chromatography (HILIC) and tandem mass spectrometry (MS/MS) with positive electrospray ionisation. The injections of volumes up to 5 μL of aqueous solutions were made possible by on-column focusing with partially filled loop injections. The fragmentation patterns for 2,4- and 2,6-toluene diamine (TDA) and 4,4′-methylene dianiline (MDA) were clarified by performing a hydrogen-deuterium exchange study.TDA and MDA were determined using trideuterated 2,4- and 2,6-TDA and dideuterated 4,4′-MDA as internal standards. Linear calibration graphs were obtained over the range 0.025-0.5μgmL<br>
                        <sup>-1</sup> with correlation coefficients &gt;0.996 and the instrumental detection limit for each compound was &lt;50fmol. The stability of the amines was influenced by the matrix, so their concentrations decreased over time.Agreement was observed between the results of analyses of PUR foam extracts by HILIC-MS/MS and results obtained by ethyl chloroformate derivatisation and reversed phase (RP) liquid chromatography-mass spectrometry (LC-MS/MS).TDA was observed to be unstable in extracts of foam but not in pure solutions. © 2010 Elsevier B.V.</p>},
  author       = {RIDDAR, JAKOB and Karlsson, Daniel and Dalene, Marianne and Skarping, Gunnar},
  issn         = {0003-2670},
  keyword      = {Hydrogen-deuterium exchange,Hydrophilic interaction liquid chromatography (HILIC),Liquid chromatography-electrospray tandem mass spectrometry,Methylene dianiline (MDA),Polyurethane (PUR) foam,Toluene diamine (TDA)},
  language     = {eng},
  number       = {1},
  pages        = {117--123},
  publisher    = {ARRAY(0x8fc5888)},
  series       = {Analytica Chimica Acta},
  title        = {Determination of aromatic amines in aqueous extracts of polyurethane foam using hydrophilic interaction liquid chromatography and mass spectrometry},
  url          = {http://dx.doi.org/10.1016/j.aca.2010.08.020},
  volume       = {678},
  year         = {2010},
}