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Long-term explant culture of rabbit flexor tendon : Effects of recombinant human insulin-like growth factor-I and serum on matrix metabolism

Abrahamsson, S. O. LU ; Lundborg, G. LU and Lohmander, L. S. (1991) In Journal of Orthopaedic Research 9(4). p.503-515
Abstract

The effects of human recombinant insulin-like growth factor-I (rhIGF-I, 50 ng/ml) on matrix metabolism in the deep flexor tendon from the tendon sheath region of the rabbit were studied in explants cultured for 3 weeks. Tendon segments cultured in medium supplemented with fetal calf serum (FCS) exhibited proliferation of the superficial cell layers. Synthesis of proteoglycan and non-collagen protein (NCP) increased threefold during the first week and remained elevated during the next 2 weeks of culture in medium supplemented with rhIGF-I or FCS, but not in medium without supplements (bovine serum albumin, BSA). The estimated halflife (t 1/2 ) for elimination of newly labeled proteoglycans from the tendon explants ranged from 5.1 to 8.5... (More)

The effects of human recombinant insulin-like growth factor-I (rhIGF-I, 50 ng/ml) on matrix metabolism in the deep flexor tendon from the tendon sheath region of the rabbit were studied in explants cultured for 3 weeks. Tendon segments cultured in medium supplemented with fetal calf serum (FCS) exhibited proliferation of the superficial cell layers. Synthesis of proteoglycan and non-collagen protein (NCP) increased threefold during the first week and remained elevated during the next 2 weeks of culture in medium supplemented with rhIGF-I or FCS, but not in medium without supplements (bovine serum albumin, BSA). The estimated halflife (t 1/2 ) for elimination of newly labeled proteoglycans from the tendon explants ranged from 5.1 to 8.5 days and from 4.9 to 6.8 days for NCP in supplemented medium. Presence of rhIGF-I or FCS did not affect degradation of matrix as compared with BSA. The total hexosamine content per tendon segment was stable during the culture period, but the non-collagen protein content decreased by 25%. Collagen synthesis decreased to 10% of the initial level after 3 weeks in supplemented medium, but to 3% in unsupplemented medium. There was no measurable turnover of collagen in explants cultured in either medium, and the collagen content remained unchanged. Our results suggest that rhIGF-I, as well as FCS, stimulates matrix synthesis but does not influence matrix turnover in rabbit flexor tendon explants in long-term culture as compared with medium without supplements. We conclude that rhIGF-I may be used as a defined growth-promoting factor in serum-free media and may be of importance in tendon healing.

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publishing date
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Contribution to journal
publication status
published
subject
in
Journal of Orthopaedic Research
volume
9
issue
4
pages
13 pages
publisher
John Wiley & Sons
external identifiers
  • Scopus:0026197319
ISSN
0736-0266
language
English
LU publication?
no
id
6c39decd-cfca-44ca-b484-14ff0a91f7f5
date added to LUP
2016-05-04 18:16:19
date last changed
2016-10-13 05:07:51
@misc{6c39decd-cfca-44ca-b484-14ff0a91f7f5,
  abstract     = {<p>The effects of human recombinant insulin-like growth factor-I (rhIGF-I, 50 ng/ml) on matrix metabolism in the deep flexor tendon from the tendon sheath region of the rabbit were studied in explants cultured for 3 weeks. Tendon segments cultured in medium supplemented with fetal calf serum (FCS) exhibited proliferation of the superficial cell layers. Synthesis of proteoglycan and non-collagen protein (NCP) increased threefold during the first week and remained elevated during the next 2 weeks of culture in medium supplemented with rhIGF-I or FCS, but not in medium without supplements (bovine serum albumin, BSA). The estimated halflife (t 1/2 ) for elimination of newly labeled proteoglycans from the tendon explants ranged from 5.1 to 8.5 days and from 4.9 to 6.8 days for NCP in supplemented medium. Presence of rhIGF-I or FCS did not affect degradation of matrix as compared with BSA. The total hexosamine content per tendon segment was stable during the culture period, but the non-collagen protein content decreased by 25%. Collagen synthesis decreased to 10% of the initial level after 3 weeks in supplemented medium, but to 3% in unsupplemented medium. There was no measurable turnover of collagen in explants cultured in either medium, and the collagen content remained unchanged. Our results suggest that rhIGF-I, as well as FCS, stimulates matrix synthesis but does not influence matrix turnover in rabbit flexor tendon explants in long-term culture as compared with medium without supplements. We conclude that rhIGF-I may be used as a defined growth-promoting factor in serum-free media and may be of importance in tendon healing.</p>},
  author       = {Abrahamsson, S. O. and Lundborg, G. and Lohmander, L. S.},
  issn         = {0736-0266},
  language     = {eng},
  number       = {4},
  pages        = {503--515},
  publisher    = {ARRAY(0x92214d8)},
  series       = {Journal of Orthopaedic Research},
  title        = {Long-term explant culture of rabbit flexor tendon : Effects of recombinant human insulin-like growth factor-I and serum on matrix metabolism},
  volume       = {9},
  year         = {1991},
}