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Differential effects of insulin-like growth factor-I on matrix and DNA synthesis in various regions and types of rabbit tendons

Abrahamsson, S. O. LU and Lohmander, S. LU (1996) In Journal of Orthopaedic Research 14(3). p.370-376
Abstract

Tendon healing and integration of tendon grafts may be site or donor specific. To determine if differences exist in sensitivity to growth factors that have the potential to influence tendon repair, we compared the effect of recombinant human insulin-like growth factor-1 on various types of tendon segments. The dose response effects on proteoglycan, collagen, noncollagen protein, and DNA synthesis were investigated in short-term explant cultures of intrasynovial intermediated and proximal segments of deep flexor tendons, extrasynovial segments of deep flexor tendons, and Achilles tendons of rabbits. The four different types of tendon segments cultured in media without recombinant human insulin-like growth factor-I synthesized similar... (More)

Tendon healing and integration of tendon grafts may be site or donor specific. To determine if differences exist in sensitivity to growth factors that have the potential to influence tendon repair, we compared the effect of recombinant human insulin-like growth factor-1 on various types of tendon segments. The dose response effects on proteoglycan, collagen, noncollagen protein, and DNA synthesis were investigated in short-term explant cultures of intrasynovial intermediated and proximal segments of deep flexor tendons, extrasynovial segments of deep flexor tendons, and Achilles tendons of rabbits. The four different types of tendon segments cultured in media without recombinant human insulin-like growth factor-I synthesized similar amounts of each of the matrix components. Intrasynovial proximal segments synthesized 15 times less DNA than other tendon segments. Recombinant human insulin-like growth factor-1 stimulated matrix and DNA synthesis of all tendon segments in a dose-dependent manner in intervals from 10 to 1,000 ng/ml. The potency (LogED50) of the stimulation did not differ between the segments. The estimated maximal stimulation (E(max)) of proteoglycan synthesis was lower, in intrasynovial proximal segments as compared with that of the other types of segments. In contrast, the estimated maximal stimulation of DNA synthesis by recombinant human insulin-like growth factor- 1 was 6-fold higher than controls in all types of tendons. These findings demonstrate differences in mitotic capacity between anatomical regions of tendons during culture without recombinant human insulin-like growth factor- 1 and in matrix synthesis after stimulant with it.

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author
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Orthopaedic Research
volume
14
issue
3
pages
7 pages
publisher
John Wiley & Sons
external identifiers
  • Scopus:0030152299
ISSN
0736-0266
DOI
10.1002/jor.1100140305
language
English
LU publication?
no
id
70369d6f-b2ef-4e9d-a949-7cb488e6c545
date added to LUP
2016-05-04 23:52:51
date last changed
2016-07-26 12:54:10
@misc{70369d6f-b2ef-4e9d-a949-7cb488e6c545,
  abstract     = {<p>Tendon healing and integration of tendon grafts may be site or donor specific. To determine if differences exist in sensitivity to growth factors that have the potential to influence tendon repair, we compared the effect of recombinant human insulin-like growth factor-1 on various types of tendon segments. The dose response effects on proteoglycan, collagen, noncollagen protein, and DNA synthesis were investigated in short-term explant cultures of intrasynovial intermediated and proximal segments of deep flexor tendons, extrasynovial segments of deep flexor tendons, and Achilles tendons of rabbits. The four different types of tendon segments cultured in media without recombinant human insulin-like growth factor-I synthesized similar amounts of each of the matrix components. Intrasynovial proximal segments synthesized 15 times less DNA than other tendon segments. Recombinant human insulin-like growth factor-1 stimulated matrix and DNA synthesis of all tendon segments in a dose-dependent manner in intervals from 10 to 1,000 ng/ml. The potency (LogED<sub>50</sub>) of the stimulation did not differ between the segments. The estimated maximal stimulation (E(max)) of proteoglycan synthesis was lower, in intrasynovial proximal segments as compared with that of the other types of segments. In contrast, the estimated maximal stimulation of DNA synthesis by recombinant human insulin-like growth factor- 1 was 6-fold higher than controls in all types of tendons. These findings demonstrate differences in mitotic capacity between anatomical regions of tendons during culture without recombinant human insulin-like growth factor- 1 and in matrix synthesis after stimulant with it.</p>},
  author       = {Abrahamsson, S. O. and Lohmander, S.},
  issn         = {0736-0266},
  language     = {eng},
  number       = {3},
  pages        = {370--376},
  publisher    = {ARRAY(0x9248110)},
  series       = {Journal of Orthopaedic Research},
  title        = {Differential effects of insulin-like growth factor-I on matrix and DNA synthesis in various regions and types of rabbit tendons},
  url          = {http://dx.doi.org/10.1002/jor.1100140305},
  volume       = {14},
  year         = {1996},
}