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Robotic implementation of a microchip-based protein clean-up and enrichment system for MALDI-TOF MS readout

Wallman, Lars LU ; Ekström, Simon LU ; Magnusson, Mattias LU ; Bolmsjö, Gunnar LU ; Olsson, Magnus LU ; Nilsson, Johan LU ; Marko-Varga, György LU and Laurell, Thomas LU (2006) In Measurement Science & Technology 17(12). p.3147-3153
Abstract
A capillary filling microfluidic proteomic sample processing system has been realized in an automated set-up. Chip-integrated solid-phase extraction is performed in a 96-array format followed by sequential capillary action elution into a piezoelectric microdispenser and subsequent transfer to MALDI targets. Samples are eluted and deposited in volumes of 200–300 nl. The robotic system offers calibration to user-defined microextraction arrays and MALDI-target formats at micrometre resolution. Built-in force feedback control ensures a precise and robust microchip docking/handling in three dimensions. An efficient automated washing protocol eliminates analyte carry-over. System throughput ranges typically from 50–100 samples h-1.
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
piezodispensing, capillary force, microfluidics, MALDI, mass spectrometry, microchip, robotic, MALDI-TOF MS, automation, protein analysis, proteomics, solid phase extraction
in
Measurement Science & Technology
volume
17
issue
12
pages
3147 - 3153
publisher
IOP Publishing
external identifiers
  • WOS:000242528500008
  • Scopus:33846110953
ISSN
0957-0233
DOI
10.1088/0957-0233/17/12/S05
language
English
LU publication?
yes
id
3cabce1b-3899-494c-99fd-26300e7488be (old id 748440)
date added to LUP
2007-12-13 14:25:07
date last changed
2016-10-13 04:34:22
@misc{3cabce1b-3899-494c-99fd-26300e7488be,
  abstract     = {A capillary filling microfluidic proteomic sample processing system has been realized in an automated set-up. Chip-integrated solid-phase extraction is performed in a 96-array format followed by sequential capillary action elution into a piezoelectric microdispenser and subsequent transfer to MALDI targets. Samples are eluted and deposited in volumes of 200–300 nl. The robotic system offers calibration to user-defined microextraction arrays and MALDI-target formats at micrometre resolution. Built-in force feedback control ensures a precise and robust microchip docking/handling in three dimensions. An efficient automated washing protocol eliminates analyte carry-over. System throughput ranges typically from 50–100 samples h-1.},
  author       = {Wallman, Lars and Ekström, Simon and Magnusson, Mattias and Bolmsjö, Gunnar and Olsson, Magnus and Nilsson, Johan and Marko-Varga, György and Laurell, Thomas},
  issn         = {0957-0233},
  keyword      = {piezodispensing,capillary force,microfluidics,MALDI,mass spectrometry,microchip,robotic,MALDI-TOF MS,automation,protein analysis,proteomics,solid phase extraction},
  language     = {eng},
  number       = {12},
  pages        = {3147--3153},
  publisher    = {ARRAY(0xa9da190)},
  series       = {Measurement Science & Technology},
  title        = {Robotic implementation of a microchip-based protein clean-up and enrichment system for MALDI-TOF MS readout},
  url          = {http://dx.doi.org/10.1088/0957-0233/17/12/S05},
  volume       = {17},
  year         = {2006},
}