Versatile microchip utilising ultrasonic standing waves
(2005) 13th Nordic Baltic Conference on Biomedical Engineering and Medical Physics p.123-124- Abstract
- This paper presents the concept and initial work on a
microfluidic platform for bead-based analysis of
biological sample. The core technology in this project
is ultrasonic manipulation and trapping of particle in
array configurations by means of acoustic forces. The
platform is ultimately aimed for parallel multistep
bioassays performed on biochemically activated
microbeads (or particles) using submicrolitre sample
volumes. A first prototype with three individually
controlled particle trapping sites has been developed
and evaluated. Standing ultrasonic waves were
generated across a microfluidic channel by integrated
PZT ultrasonic... (More) - This paper presents the concept and initial work on a
microfluidic platform for bead-based analysis of
biological sample. The core technology in this project
is ultrasonic manipulation and trapping of particle in
array configurations by means of acoustic forces. The
platform is ultimately aimed for parallel multistep
bioassays performed on biochemically activated
microbeads (or particles) using submicrolitre sample
volumes. A first prototype with three individually
controlled particle trapping sites has been developed
and evaluated. Standing ultrasonic waves were
generated across a microfluidic channel by integrated
PZT ultrasonic microtransducers. Particles in a fluid
passing a transducer were drawn to pressure minima
in the acoustic field, thereby being trapped and
confined laterally over the transducer. It is
anticipated that acoustic trapping using integrated
transducers can be exploited in miniaturised total
chemical analysis systems (µTAS), where e.g.
microbeads with immobilised antibodies can be
trapped in arrays and subjected to minute amounts of
sample followed by a reaction, detected using
fluorescence. Preliminary results indicate that the
platform is capable of handling live cells as well as
microbeads. A first model bioassay with detection of
fluorescein marked avidin binding to trapped biotin
beads has been evaluated. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/789762
- author
- Evander, Mikael LU ; Lilliehorn, Tobias ; Johansson, Linda ; Almqvist, Monica LU ; Simu, Urban ; Johansson, Stefan ; Laurell, Thomas LU and Nilsson, Johan LU
- organization
- publishing date
- 2005
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- host publication
- IFMBE Proceedings 2005
- pages
- 2 pages
- conference name
- 13th Nordic Baltic Conference on Biomedical Engineering and Medical Physics
- conference dates
- 2005-06-13 - 2006-06-15
- language
- English
- LU publication?
- yes
- id
- 13ae3d63-a1ef-4bd5-ae0d-bed9f4e6a222 (old id 789762)
- date added to LUP
- 2016-04-04 14:20:48
- date last changed
- 2018-11-21 21:19:46
@inproceedings{13ae3d63-a1ef-4bd5-ae0d-bed9f4e6a222,
abstract = {{This paper presents the concept and initial work on a<br/><br>
microfluidic platform for bead-based analysis of<br/><br>
biological sample. The core technology in this project<br/><br>
is ultrasonic manipulation and trapping of particle in<br/><br>
array configurations by means of acoustic forces. The<br/><br>
platform is ultimately aimed for parallel multistep<br/><br>
bioassays performed on biochemically activated<br/><br>
microbeads (or particles) using submicrolitre sample<br/><br>
volumes. A first prototype with three individually<br/><br>
controlled particle trapping sites has been developed<br/><br>
and evaluated. Standing ultrasonic waves were<br/><br>
generated across a microfluidic channel by integrated<br/><br>
PZT ultrasonic microtransducers. Particles in a fluid<br/><br>
passing a transducer were drawn to pressure minima<br/><br>
in the acoustic field, thereby being trapped and<br/><br>
confined laterally over the transducer. It is<br/><br>
anticipated that acoustic trapping using integrated<br/><br>
transducers can be exploited in miniaturised total<br/><br>
chemical analysis systems (µTAS), where e.g.<br/><br>
microbeads with immobilised antibodies can be<br/><br>
trapped in arrays and subjected to minute amounts of<br/><br>
sample followed by a reaction, detected using<br/><br>
fluorescence. Preliminary results indicate that the<br/><br>
platform is capable of handling live cells as well as<br/><br>
microbeads. A first model bioassay with detection of<br/><br>
fluorescein marked avidin binding to trapped biotin<br/><br>
beads has been evaluated.}},
author = {{Evander, Mikael and Lilliehorn, Tobias and Johansson, Linda and Almqvist, Monica and Simu, Urban and Johansson, Stefan and Laurell, Thomas and Nilsson, Johan}},
booktitle = {{IFMBE Proceedings 2005}},
language = {{eng}},
pages = {{123--124}},
title = {{Versatile microchip utilising ultrasonic standing waves}},
year = {{2005}},
}