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Affinity partitioning of biotinylated mixed liposomes : effect of charge on biotin--NeutrAvidin interaction

Barinaga-Rementeria Ramírez, I LU ; Ekblad, L LU and Jergil, B LU (2000) In Journal of Chromatography. B, Biomedical Sciences and Applications 743(1-2). p.96-389
Abstract

The partitioning behaviour of biotinylated mixed liposomes in aqueous poly(ethylene glycol)/dextran two-phase systems containing NeutrAvidin-dextran suggests that the biotin-NeutrAvidin affinity interaction is charge dependent. Biotinylated phosphatidylcholine liposomes with a low negative surface charge distributed in the NeutrAvidin-containing bottom phase at neutral pH, but the introduction of additional negative charges by including phosphatidylserine or the surfactant sodium dodecylsulfate in the liposomes caused them to distribute in the poly(ethylene glycol)-rich top phase instead. By gradually lowering the pH of the affinity two-phase system below the isoelectric point (6.3) of NeutrAvidin, negatively charged... (More)

The partitioning behaviour of biotinylated mixed liposomes in aqueous poly(ethylene glycol)/dextran two-phase systems containing NeutrAvidin-dextran suggests that the biotin-NeutrAvidin affinity interaction is charge dependent. Biotinylated phosphatidylcholine liposomes with a low negative surface charge distributed in the NeutrAvidin-containing bottom phase at neutral pH, but the introduction of additional negative charges by including phosphatidylserine or the surfactant sodium dodecylsulfate in the liposomes caused them to distribute in the poly(ethylene glycol)-rich top phase instead. By gradually lowering the pH of the affinity two-phase system below the isoelectric point (6.3) of NeutrAvidin, negatively charged phosphatidylserine/phosphatidylcholine liposomes increasingly were attracted by NeutrAvidin to the bottom phase. It is suggested that acidic amino acids present at the rim of the biotin-binding pocket of NeutrAvidin may interact electrostatically with charged residues of the closely apposed liposome surface affecting the affinity interaction.

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author
organization
publishing date
type
Contribution to journal
publication status
published
keywords
Avidin, Biotin, Chromatography, Affinity, Hydrogen-Ion Concentration, Liposomes, Phosphatidylcholines, Surface-Active Agents
in
Journal of Chromatography. B, Biomedical Sciences and Applications
volume
743
issue
1-2
pages
8 pages
publisher
Elsevier
external identifiers
  • Scopus:0343517652
ISSN
1387-2273
language
English
LU publication?
yes
id
78b298a0-9ac9-479e-91ba-736f024c344b
date added to LUP
2016-08-29 15:33:03
date last changed
2016-10-13 05:12:55
@misc{78b298a0-9ac9-479e-91ba-736f024c344b,
  abstract     = {<p>The partitioning behaviour of biotinylated mixed liposomes in aqueous poly(ethylene glycol)/dextran two-phase systems containing NeutrAvidin-dextran suggests that the biotin-NeutrAvidin affinity interaction is charge dependent. Biotinylated phosphatidylcholine liposomes with a low negative surface charge distributed in the NeutrAvidin-containing bottom phase at neutral pH, but the introduction of additional negative charges by including phosphatidylserine or the surfactant sodium dodecylsulfate in the liposomes caused them to distribute in the poly(ethylene glycol)-rich top phase instead. By gradually lowering the pH of the affinity two-phase system below the isoelectric point (6.3) of NeutrAvidin, negatively charged phosphatidylserine/phosphatidylcholine liposomes increasingly were attracted by NeutrAvidin to the bottom phase. It is suggested that acidic amino acids present at the rim of the biotin-binding pocket of NeutrAvidin may interact electrostatically with charged residues of the closely apposed liposome surface affecting the affinity interaction.</p>},
  author       = {Barinaga-Rementeria Ramírez, I and Ekblad, L and Jergil, B},
  issn         = {1387-2273},
  keyword      = {Avidin,Biotin,Chromatography, Affinity,Hydrogen-Ion Concentration,Liposomes,Phosphatidylcholines,Surface-Active Agents},
  language     = {eng},
  month        = {06},
  number       = {1-2},
  pages        = {96--389},
  publisher    = {ARRAY(0x8709b30)},
  series       = {Journal of Chromatography. B, Biomedical Sciences and Applications},
  title        = {Affinity partitioning of biotinylated mixed liposomes : effect of charge on biotin--NeutrAvidin interaction},
  volume       = {743},
  year         = {2000},
}