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Mouse monoclonal antibodies against outer membrane proteins of a vaccine strain of Neisseria meningitidis B : 4:P1.15

Cruz, Silian; Musacchio, Alexis; Fernàndez-De-Cossio, Maria E.; Ohlin, Mats LU ; Nazábal, Consuelo; Freyre, Freya; Borrebaeck, C. A K LU and Gavilondo, Jorge V. (1998) In Minerva Biotecnologica 10(2). p.65-70
Abstract

Background. Neisseria meningitidis (Nm) is a Gram negative diplococcus causing bacterial meningitis and fulminant septicemia. In order to allow efficient characterization of infecting strains, antibody reagents for use as analytical tools have proven to be invaluable tools. Similarly, antibodies against relevant bacterial antigens may guide in the selection of components to be included in developing vaccine strategies. Methods. We have thus developed mouse monoclonal antibodies specific for class 1, 3 and 5 antigens expressed by the B:4:P1.15 isolate CU385/83, also being used in a recently developed protective vaccine. In particular, two antibodies CB-Nm.1 and CB- Nm.2 recognize epitopes partly overlapping the subserotype (class 1... (More)

Background. Neisseria meningitidis (Nm) is a Gram negative diplococcus causing bacterial meningitis and fulminant septicemia. In order to allow efficient characterization of infecting strains, antibody reagents for use as analytical tools have proven to be invaluable tools. Similarly, antibodies against relevant bacterial antigens may guide in the selection of components to be included in developing vaccine strategies. Methods. We have thus developed mouse monoclonal antibodies specific for class 1, 3 and 5 antigens expressed by the B:4:P1.15 isolate CU385/83, also being used in a recently developed protective vaccine. In particular, two antibodies CB-Nm.1 and CB- Nm.2 recognize epitopes partly overlapping the subserotype (class 1 antigens) and serotype (class 3 antigen) specificities detected by the previously defined antibodies C6 and 15-1-P4 respectively, were evaluated. Results. As judged by strain recognition, the absolute requirement for binding differs between both the class 1-specific and class 3 specific antibodies suggesting the importance of using multiple antibodies when evaluating subserotype/serotype characteristics of clinical isolates of Nm by serological methods. Conclusion. Furthermore, the development of antibodies crossreactive with subserotype/serotype antigens may partly explain the ability of outer membrane protein vaccine to induce protective activity against strains considered as carrying different class 1 and 3 antigens as determined by available (sub)serotyping reagents.

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author
organization
publishing date
type
Contribution to journal
publication status
published
keywords
Antibodies, monoclonal, Bacterial outer membrane proteins, Neisseria meningitidis
in
Minerva Biotecnologica
volume
10
issue
2
pages
6 pages
publisher
Edizioni Minerva Medica S.p.A.
external identifiers
  • Scopus:0031771448
ISSN
1120-4826
language
English
LU publication?
yes
id
7b8552b5-4189-419a-aeb3-e2bc1d202441
date added to LUP
2016-04-19 14:06:58
date last changed
2016-04-22 12:50:28
@misc{7b8552b5-4189-419a-aeb3-e2bc1d202441,
  abstract     = {<p>Background. Neisseria meningitidis (Nm) is a Gram negative diplococcus causing bacterial meningitis and fulminant septicemia. In order to allow efficient characterization of infecting strains, antibody reagents for use as analytical tools have proven to be invaluable tools. Similarly, antibodies against relevant bacterial antigens may guide in the selection of components to be included in developing vaccine strategies. Methods. We have thus developed mouse monoclonal antibodies specific for class 1, 3 and 5 antigens expressed by the B:4:P1.15 isolate CU385/83, also being used in a recently developed protective vaccine. In particular, two antibodies CB-Nm.1 and CB- Nm.2 recognize epitopes partly overlapping the subserotype (class 1 antigens) and serotype (class 3 antigen) specificities detected by the previously defined antibodies C6 and 15-1-P4 respectively, were evaluated. Results. As judged by strain recognition, the absolute requirement for binding differs between both the class 1-specific and class 3 specific antibodies suggesting the importance of using multiple antibodies when evaluating subserotype/serotype characteristics of clinical isolates of Nm by serological methods. Conclusion. Furthermore, the development of antibodies crossreactive with subserotype/serotype antigens may partly explain the ability of outer membrane protein vaccine to induce protective activity against strains considered as carrying different class 1 and 3 antigens as determined by available (sub)serotyping reagents.</p>},
  author       = {Cruz, Silian and Musacchio, Alexis and Fernàndez-De-Cossio, Maria E. and Ohlin, Mats and Nazábal, Consuelo and Freyre, Freya and Borrebaeck, C. A K and Gavilondo, Jorge V.},
  issn         = {1120-4826},
  keyword      = {Antibodies, monoclonal,Bacterial outer membrane proteins,Neisseria meningitidis},
  language     = {eng},
  number       = {2},
  pages        = {65--70},
  publisher    = {ARRAY(0x8b65870)},
  series       = {Minerva Biotecnologica},
  title        = {Mouse monoclonal antibodies against outer membrane proteins of a vaccine strain of Neisseria meningitidis B : 4:P1.15},
  volume       = {10},
  year         = {1998},
}