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(1)H and hyperpolarized (3)He MR imaging of mouse with LPS-induced inflammation

Olsson, Lars E LU ; Smailagic, Amir; Onnervik, Per-Ola and Hockings, Paul D (2009) In Journal of Magnetic Resonance Imaging 29(4). p.81-977
Abstract

PURPOSE: To evaluate the lipopolysaccharide (LPS) model of chronic obstructive pulmonary disease (COPD) in mouse with (1)H and hyperpolarized (HP) (3)He MR imaging.

MATERIALS AND METHODS: Axial slices of the lung volume were acquired with HP (3)He and (1)H MRI at 4, 24, and 48 h after LPS exposure. A quantitative ventilation index was calculated from two HP (3)He acquisitions. A bronchoalveolar lavage (BAL) for a cell count was performed following magnetic resonance imaging (MRI).

RESULTS: The LPS exposure resulted in a significant increase of cells in BAL, with maximum at 48 h. Lesions on (3)He images were characterized by ventilation defects, whereas lesions on (1)H images were hyperintense and were attributed to edema.... (More)

PURPOSE: To evaluate the lipopolysaccharide (LPS) model of chronic obstructive pulmonary disease (COPD) in mouse with (1)H and hyperpolarized (HP) (3)He MR imaging.

MATERIALS AND METHODS: Axial slices of the lung volume were acquired with HP (3)He and (1)H MRI at 4, 24, and 48 h after LPS exposure. A quantitative ventilation index was calculated from two HP (3)He acquisitions. A bronchoalveolar lavage (BAL) for a cell count was performed following magnetic resonance imaging (MRI).

RESULTS: The LPS exposure resulted in a significant increase of cells in BAL, with maximum at 48 h. Lesions on (3)He images were characterized by ventilation defects, whereas lesions on (1)H images were hyperintense and were attributed to edema. The number of lesions was at maximum at 48 h. At this time point, and for both (3)He and (1)H MRI, the volume of the lesions was significantly higher for LPS-exposed mice compared to controls. At 4, 24, and 48 h the ventilation index from the (3)He data was significantly smaller for the LPS-exposed animals compared to controls.

CONCLUSION: The time point 48 h after LPS exposure was advantageous for MRI evaluation. Functional read-out with (3)He MRI seems to be more sensitive than conventional (1)H MRI.

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author
publishing date
type
Contribution to journal
publication status
published
keywords
Animals, Bronchoalveolar Lavage, Female, Helium, Hydrogen, Isotopes, Lipopolysaccharides, Magnetic Resonance Imaging, Mice, Mice, Inbred BALB C, Pulmonary Disease, Chronic Obstructive
in
Journal of Magnetic Resonance Imaging
volume
29
issue
4
pages
5 pages
publisher
John Wiley & Sons
external identifiers
  • Scopus:65249098357
ISSN
1053-1807
DOI
10.1002/jmri.21728
language
English
LU publication?
no
id
7ed1b8f9-abcc-426f-a1a7-4996cc8dddfa
date added to LUP
2016-08-16 12:11:06
date last changed
2016-11-17 13:04:19
@misc{7ed1b8f9-abcc-426f-a1a7-4996cc8dddfa,
  abstract     = {<p>PURPOSE: To evaluate the lipopolysaccharide (LPS) model of chronic obstructive pulmonary disease (COPD) in mouse with (1)H and hyperpolarized (HP) (3)He MR imaging.</p><p>MATERIALS AND METHODS: Axial slices of the lung volume were acquired with HP (3)He and (1)H MRI at 4, 24, and 48 h after LPS exposure. A quantitative ventilation index was calculated from two HP (3)He acquisitions. A bronchoalveolar lavage (BAL) for a cell count was performed following magnetic resonance imaging (MRI).</p><p>RESULTS: The LPS exposure resulted in a significant increase of cells in BAL, with maximum at 48 h. Lesions on (3)He images were characterized by ventilation defects, whereas lesions on (1)H images were hyperintense and were attributed to edema. The number of lesions was at maximum at 48 h. At this time point, and for both (3)He and (1)H MRI, the volume of the lesions was significantly higher for LPS-exposed mice compared to controls. At 4, 24, and 48 h the ventilation index from the (3)He data was significantly smaller for the LPS-exposed animals compared to controls.</p><p>CONCLUSION: The time point 48 h after LPS exposure was advantageous for MRI evaluation. Functional read-out with (3)He MRI seems to be more sensitive than conventional (1)H MRI.</p>},
  author       = {Olsson, Lars E and Smailagic, Amir and Onnervik, Per-Ola and Hockings, Paul D},
  issn         = {1053-1807},
  keyword      = {Animals,Bronchoalveolar Lavage,Female,Helium,Hydrogen,Isotopes,Lipopolysaccharides,Magnetic Resonance Imaging,Mice,Mice, Inbred BALB C,Pulmonary Disease, Chronic Obstructive},
  language     = {eng},
  number       = {4},
  pages        = {81--977},
  publisher    = {ARRAY(0xc3ec6a0)},
  series       = {Journal of Magnetic Resonance Imaging},
  title        = {(1)H and hyperpolarized (3)He MR imaging of mouse with LPS-induced inflammation},
  url          = {http://dx.doi.org/10.1002/jmri.21728},
  volume       = {29},
  year         = {2009},
}